Inhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitro
Aim. In vitro analysis of the inhibitory activity of aqueous extracts of ready‐made tea compositions and their constituent ingredients on SARSCOV‐ 2 replication.Material and Methods. The laboratory strain SARS‐CoV‐ 2/human/RUS/Nsk‐FRCFTM‐1/2020 was passivated on Vero cell culture. For the preparatio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Kamerton
2022-07-01
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| Series: | Юг России: экология, развитие |
| Subjects: | |
| Online Access: | https://ecodag.elpub.ru/ugro/article/view/2488 |
| _version_ | 1797880530487738368 |
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| author | Е. I. Каzachinskaia А. А. Chepurnov Yu. V. Коnonova А. А. Shelemba V. V. Romanyuk M. G. Magomedov А. М. Shestopalov |
| author_facet | Е. I. Каzachinskaia А. А. Chepurnov Yu. V. Коnonova А. А. Shelemba V. V. Romanyuk M. G. Magomedov А. М. Shestopalov |
| author_sort | Е. I. Каzachinskaia |
| collection | DOAJ |
| description | Aim. In vitro analysis of the inhibitory activity of aqueous extracts of ready‐made tea compositions and their constituent ingredients on SARSCOV‐ 2 replication.Material and Methods. The laboratory strain SARS‐CoV‐ 2/human/RUS/Nsk‐FRCFTM‐1/2020 was passivated on Vero cell culture. For the preparation of water extracts six types of experimental tea compositions (not containing flavorings) based on black and green tea or flowers of hibiscus as well as individual ingredients were used. Antiviral (inhibitory) activity of plant raw materials was studied in vitro according to the classical scheme of neutralization (inactivation) of the virus.Results. When compared with the main control sample of an aqueous extract of chaga (Inonotus obliquus) with a 50% effective concentration equal to 13.72±2.99 μg/ml against 103 TCPD50/ml of SARS‐CoV‐2, superior activity was found for extracts of the spice Syzygium aromaticum L. and black tea from Nepal. Approximately equal inhibitory activity was detected for extracts of tea compositions based on black tea with the addition of the grass Thymus altaicus and the spice S. aromaticum L. or with the addition of seeds of Carum carvi L. and the root of Angelica archangelica L. In addition, such activity was detected for extracts of tea compositions based on green tea from China with the addition of leaves of Mentha piperita L. and flowers of Lavandula angustifolia MILL. or with the addition of the peel of Citrus sinensis L. and seeds of Pimpinella anisum L. Extracts from individual plant raw materials used to make tea compositions were black teas from India, Argentina, Vietnam and Sri Lanka, leaves of M. piperita L. and flowers of L. angustifolia MILL. Cetraria islandica lichen, green tea and berries of Oxycoccus also showed antiviral activity.Conclusion. The results obtained suggest that tea compositions based on black and green tea with the addition of various plant raw materials in the form of a regular drink can be useful to people with COVID‐19 infection. |
| first_indexed | 2024-04-10T03:05:40Z |
| format | Article |
| id | doaj.art-60f04e0fd587408b81e79d83d2f63ab7 |
| institution | Directory Open Access Journal |
| issn | 1992-1098 2413-0958 |
| language | Russian |
| last_indexed | 2024-04-10T03:05:40Z |
| publishDate | 2022-07-01 |
| publisher | Kamerton |
| record_format | Article |
| series | Юг России: экология, развитие |
| spelling | doaj.art-60f04e0fd587408b81e79d83d2f63ab72023-03-13T07:36:03ZrusKamertonЮг России: экология, развитие1992-10982413-09582022-07-01172769010.18470/1992-1098-2022-2-76-901239Inhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitroЕ. I. Каzachinskaia0А. А. Chepurnov1Yu. V. Коnonova2А. А. Shelemba3V. V. Romanyuk4M. G. Magomedov5А. М. Shestopalov6Научно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины» (ФИЦ ФТМ СО РАН) Cибирского отделения Российской Академии наукНаучно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины» (ФИЦ ФТМ СО РАН) Cибирского отделения Российской Академии наукНаучно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины» (ФИЦ ФТМ СО РАН) Cибирского отделения Российской Академии наукНаучно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины» (ФИЦ ФТМ СО РАН) Cибирского отделения Российской Академии наукООО Научно‐производственная фирма (НПФ) «Золотая долина»Дагестанский государственный медицинский университетНаучно‐исследовательский институт вирусологии Федерального исследовательского центра фундаментальной и трансляционной медицины» (ФИЦ ФТМ СО РАН) Cибирского отделения Российской Академии наукAim. In vitro analysis of the inhibitory activity of aqueous extracts of ready‐made tea compositions and their constituent ingredients on SARSCOV‐ 2 replication.Material and Methods. The laboratory strain SARS‐CoV‐ 2/human/RUS/Nsk‐FRCFTM‐1/2020 was passivated on Vero cell culture. For the preparation of water extracts six types of experimental tea compositions (not containing flavorings) based on black and green tea or flowers of hibiscus as well as individual ingredients were used. Antiviral (inhibitory) activity of plant raw materials was studied in vitro according to the classical scheme of neutralization (inactivation) of the virus.Results. When compared with the main control sample of an aqueous extract of chaga (Inonotus obliquus) with a 50% effective concentration equal to 13.72±2.99 μg/ml against 103 TCPD50/ml of SARS‐CoV‐2, superior activity was found for extracts of the spice Syzygium aromaticum L. and black tea from Nepal. Approximately equal inhibitory activity was detected for extracts of tea compositions based on black tea with the addition of the grass Thymus altaicus and the spice S. aromaticum L. or with the addition of seeds of Carum carvi L. and the root of Angelica archangelica L. In addition, such activity was detected for extracts of tea compositions based on green tea from China with the addition of leaves of Mentha piperita L. and flowers of Lavandula angustifolia MILL. or with the addition of the peel of Citrus sinensis L. and seeds of Pimpinella anisum L. Extracts from individual plant raw materials used to make tea compositions were black teas from India, Argentina, Vietnam and Sri Lanka, leaves of M. piperita L. and flowers of L. angustifolia MILL. Cetraria islandica lichen, green tea and berries of Oxycoccus also showed antiviral activity.Conclusion. The results obtained suggest that tea compositions based on black and green tea with the addition of various plant raw materials in the form of a regular drink can be useful to people with COVID‐19 infection.https://ecodag.elpub.ru/ugro/article/view/2488чайные композициирастительное сырьеантивирусная активностьsars‐cov‐2 |
| spellingShingle | Е. I. Каzachinskaia А. А. Chepurnov Yu. V. Коnonova А. А. Shelemba V. V. Romanyuk M. G. Magomedov А. М. Shestopalov Inhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitro Юг России: экология, развитие чайные композиции растительное сырье антивирусная активность sars‐cov‐2 |
| title | Inhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitro |
| title_full | Inhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitro |
| title_fullStr | Inhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitro |
| title_full_unstemmed | Inhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitro |
| title_short | Inhibitory activity of tea compositions and their constituent ingredients on SARS‐COV‐2 replication in vitro |
| title_sort | inhibitory activity of tea compositions and their constituent ingredients on sars cov 2 replication in vitro |
| topic | чайные композиции растительное сырье антивирусная активность sars‐cov‐2 |
| url | https://ecodag.elpub.ru/ugro/article/view/2488 |
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