Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil.
There is a constant need to find feed additives that improve health and nutrition of farmed fish and lessen the intestinal inflammation induced by plant-based ingredients. The objective of this study was to evaluate the effects of adding an organic acid salt to alleviate some of the detrimental effe...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5127657?pdf=render |
| _version_ | 1819086017432387584 |
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| author | Itziar Estensoro Gabriel Ballester-Lozano Laura Benedito-Palos Fabian Grammes Juan Antonio Martos-Sitcha Liv-Torunn Mydland Josep Alvar Calduch-Giner Juan Fuentes Vasileios Karalazos Álvaro Ortiz Margareth Øverland Ariadna Sitjà-Bobadilla Jaume Pérez-Sánchez |
| author_facet | Itziar Estensoro Gabriel Ballester-Lozano Laura Benedito-Palos Fabian Grammes Juan Antonio Martos-Sitcha Liv-Torunn Mydland Josep Alvar Calduch-Giner Juan Fuentes Vasileios Karalazos Álvaro Ortiz Margareth Øverland Ariadna Sitjà-Bobadilla Jaume Pérez-Sánchez |
| author_sort | Itziar Estensoro |
| collection | DOAJ |
| description | There is a constant need to find feed additives that improve health and nutrition of farmed fish and lessen the intestinal inflammation induced by plant-based ingredients. The objective of this study was to evaluate the effects of adding an organic acid salt to alleviate some of the detrimental effects of extreme plant-ingredient substitution of fish meal (FM) and fish oil (FO) in gilthead sea bream diet. Three experiments were conducted. In a first trial (T1), the best dose (0.4%) of sodium butyrate (BP-70 ®NOREL) was chosen after a short (9-weeks) feeding period. In a second longer trial (T2) (8 months), four diets were used: a control diet containing 25% FM (T2-D1) and three experimental diets containing 5% FM (T2-D2, T2-D3, T2-D4). FO was the only added oil in D1, while a blend of plant oils replaced 58% and 84% of FO in T2-D2, and T2-D3 and T2-D4, respectively. The latter was supplemented with 0.4% BP-70. In a third trial (T3), two groups of fish were fed for 12 and 38 months with D1, D3 and D4 diets of T2. The effects of dietary changes were studied using histochemical, immunohistochemical, molecular and electrophysiological tools. The extreme diet (T2-D3) modified significantly the transcriptomic profile, especially at the anterior intestine, up-regulating the expression of inflammatory markers, in coincidence with a higher presence of granulocytes and lymphocytes in the submucosa, and changing genes involved in antioxidant defences, epithelial permeability and mucus production. Trans-epithelial electrical resistance (Rt) was also decreased (T3-D3). Most of these modifications were returned to control values with the addition of BP-70. None of the experimental diets modified the staining pattern of PCNA, FABP2 or ALPI. These results further confirm the potential of this additive to improve or reverse the detrimental effects of extreme fish diet formulations. |
| first_indexed | 2024-12-21T21:13:34Z |
| format | Article |
| id | doaj.art-60f0cb0cc38549969f2b8244f10f733b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T21:13:34Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-60f0cb0cc38549969f2b8244f10f733b2022-12-21T18:50:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011111e016656410.1371/journal.pone.0166564Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil.Itziar EstensoroGabriel Ballester-LozanoLaura Benedito-PalosFabian GrammesJuan Antonio Martos-SitchaLiv-Torunn MydlandJosep Alvar Calduch-GinerJuan FuentesVasileios KaralazosÁlvaro OrtizMargareth ØverlandAriadna Sitjà-BobadillaJaume Pérez-SánchezThere is a constant need to find feed additives that improve health and nutrition of farmed fish and lessen the intestinal inflammation induced by plant-based ingredients. The objective of this study was to evaluate the effects of adding an organic acid salt to alleviate some of the detrimental effects of extreme plant-ingredient substitution of fish meal (FM) and fish oil (FO) in gilthead sea bream diet. Three experiments were conducted. In a first trial (T1), the best dose (0.4%) of sodium butyrate (BP-70 ®NOREL) was chosen after a short (9-weeks) feeding period. In a second longer trial (T2) (8 months), four diets were used: a control diet containing 25% FM (T2-D1) and three experimental diets containing 5% FM (T2-D2, T2-D3, T2-D4). FO was the only added oil in D1, while a blend of plant oils replaced 58% and 84% of FO in T2-D2, and T2-D3 and T2-D4, respectively. The latter was supplemented with 0.4% BP-70. In a third trial (T3), two groups of fish were fed for 12 and 38 months with D1, D3 and D4 diets of T2. The effects of dietary changes were studied using histochemical, immunohistochemical, molecular and electrophysiological tools. The extreme diet (T2-D3) modified significantly the transcriptomic profile, especially at the anterior intestine, up-regulating the expression of inflammatory markers, in coincidence with a higher presence of granulocytes and lymphocytes in the submucosa, and changing genes involved in antioxidant defences, epithelial permeability and mucus production. Trans-epithelial electrical resistance (Rt) was also decreased (T3-D3). Most of these modifications were returned to control values with the addition of BP-70. None of the experimental diets modified the staining pattern of PCNA, FABP2 or ALPI. These results further confirm the potential of this additive to improve or reverse the detrimental effects of extreme fish diet formulations.http://europepmc.org/articles/PMC5127657?pdf=render |
| spellingShingle | Itziar Estensoro Gabriel Ballester-Lozano Laura Benedito-Palos Fabian Grammes Juan Antonio Martos-Sitcha Liv-Torunn Mydland Josep Alvar Calduch-Giner Juan Fuentes Vasileios Karalazos Álvaro Ortiz Margareth Øverland Ariadna Sitjà-Bobadilla Jaume Pérez-Sánchez Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil. PLoS ONE |
| title | Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil. |
| title_full | Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil. |
| title_fullStr | Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil. |
| title_full_unstemmed | Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil. |
| title_short | Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil. |
| title_sort | dietary butyrate helps to restore the intestinal status of a marine teleost sparus aurata fed extreme diets low in fish meal and fish oil |
| url | http://europepmc.org/articles/PMC5127657?pdf=render |
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