Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms
Volume resuscitation is an important early treatment for haemorrhagic shock. Haemoglobin-based oxygen carrier (HBOC) can expand the volume and provide oxygen for tissues. Vascular leakage is common complication in the process of haemorrhagic shock and resuscitation. The aim of this study was to obse...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1835937 |
| _version_ | 1827181465522143232 |
|---|---|
| author | Hong Liang Zhao Jie Zhang Yu Zhu Yue Wu Qing Guang Yan Xiao Yong Peng Xin Ming Xiang Kun Lun Tian Tao Li Liang Ming Liu |
| author_facet | Hong Liang Zhao Jie Zhang Yu Zhu Yue Wu Qing Guang Yan Xiao Yong Peng Xin Ming Xiang Kun Lun Tian Tao Li Liang Ming Liu |
| author_sort | Hong Liang Zhao |
| collection | DOAJ |
| description | Volume resuscitation is an important early treatment for haemorrhagic shock. Haemoglobin-based oxygen carrier (HBOC) can expand the volume and provide oxygen for tissues. Vascular leakage is common complication in the process of haemorrhagic shock and resuscitation. The aim of this study was to observe the effects of HBOC (a bovine-derived, cross-linked tetramer haemoglobin oxygen-carrying solution, 0.5 g/L) on vascular leakage in rats after haemorrhagic shock. A haemorrhagic shock rat model and hypoxic vascular endothelial cells (VECs) were used. The role of intercellular junctions and endothelial glycocalyx in the protective effects of HBOC and the relationship with mitochondrial function were analysed. After haemorrhagic shock, the pulmonary vascular permeability to FITC-BSA, Evans Blue was increased, endothelial glycocalyx was destroyed and the expression of intercellular junction proteins was decreased. After haemorrhagic shock, a small volume of HBOC solution (6 ml/kg) protected pulmonary vascular permeability, increased structural thickness of endothelial glycocalyx, the levels of its components and increased expression levels of the intercellular junction proteins ZO-1, VE-cadherin and occludin. Moreover, HBOC significantly increased oxygen delivery and consumption in rats, improved VEC mitochondrial function and structure. In conclusion, HBOC mitigates endothelial leakage by protecting endothelial glycocalyx and intercellular junctions through improving mitochondrial function and tissue oxygen delivery. |
| first_indexed | 2024-03-13T01:40:44Z |
| format | Article |
| id | doaj.art-60f0cd0ef11640a5b8252bd5e2f42b91 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2025-03-21T05:47:15Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-60f0cd0ef11640a5b8252bd5e2f42b912024-07-23T18:44:31ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-014811272128110.1080/21691401.2020.1835937Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanismsHong Liang Zhao0Jie Zhang1Yu Zhu2Yue Wu3Qing Guang Yan4Xiao Yong Peng5Xin Ming Xiang6Kun Lun Tian7Tao Li8Liang Ming Liu9State Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaState Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. ChinaVolume resuscitation is an important early treatment for haemorrhagic shock. Haemoglobin-based oxygen carrier (HBOC) can expand the volume and provide oxygen for tissues. Vascular leakage is common complication in the process of haemorrhagic shock and resuscitation. The aim of this study was to observe the effects of HBOC (a bovine-derived, cross-linked tetramer haemoglobin oxygen-carrying solution, 0.5 g/L) on vascular leakage in rats after haemorrhagic shock. A haemorrhagic shock rat model and hypoxic vascular endothelial cells (VECs) were used. The role of intercellular junctions and endothelial glycocalyx in the protective effects of HBOC and the relationship with mitochondrial function were analysed. After haemorrhagic shock, the pulmonary vascular permeability to FITC-BSA, Evans Blue was increased, endothelial glycocalyx was destroyed and the expression of intercellular junction proteins was decreased. After haemorrhagic shock, a small volume of HBOC solution (6 ml/kg) protected pulmonary vascular permeability, increased structural thickness of endothelial glycocalyx, the levels of its components and increased expression levels of the intercellular junction proteins ZO-1, VE-cadherin and occludin. Moreover, HBOC significantly increased oxygen delivery and consumption in rats, improved VEC mitochondrial function and structure. In conclusion, HBOC mitigates endothelial leakage by protecting endothelial glycocalyx and intercellular junctions through improving mitochondrial function and tissue oxygen delivery.https://www.tandfonline.com/doi/10.1080/21691401.2020.1835937Endothelial glycocalyxHBOChaemorrhagic shocklung injuryvascular leakage |
| spellingShingle | Hong Liang Zhao Jie Zhang Yu Zhu Yue Wu Qing Guang Yan Xiao Yong Peng Xin Ming Xiang Kun Lun Tian Tao Li Liang Ming Liu Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms Artificial Cells, Nanomedicine, and Biotechnology Endothelial glycocalyx HBOC haemorrhagic shock lung injury vascular leakage |
| title | Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms |
| title_full | Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms |
| title_fullStr | Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms |
| title_full_unstemmed | Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms |
| title_short | Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms |
| title_sort | protective effects of hboc on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms |
| topic | Endothelial glycocalyx HBOC haemorrhagic shock lung injury vascular leakage |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1835937 |
| work_keys_str_mv | AT hongliangzhao protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT jiezhang protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT yuzhu protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT yuewu protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT qingguangyan protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT xiaoyongpeng protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT xinmingxiang protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT kunluntian protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT taoli protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms AT liangmingliu protectiveeffectsofhboconpulmonaryvascularleakageafterhaemorrhagicshockandtheunderlyingmechanisms |