Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana
Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses, such as drought, heat, cold, water logging and phytopathogens. Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement. Here we re...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-02-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311918621276 |
| _version_ | 1819011900530229248 |
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| author | Ya-bin ZHANG Wei TANG Li-huan WANG Ya-wen HU Xian-wen LIU Yong-sheng LIU |
| author_facet | Ya-bin ZHANG Wei TANG Li-huan WANG Ya-wen HU Xian-wen LIU Yong-sheng LIU |
| author_sort | Ya-bin ZHANG |
| collection | DOAJ |
| description | Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses, such as drought, heat, cold, water logging and phytopathogens. Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement. Here we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog (AcMYB3R) whose expression was induced by drought, salinity and cold stress. In vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene KNOLLE. The Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt stress. The expressions of stress-responsive genes such as RD29A, RD29B, COR15A and RD22 were prominently up-regulated by ectopic expression of AcMYB3R. Our study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production. |
| first_indexed | 2024-12-21T01:35:30Z |
| format | Article |
| id | doaj.art-60f1e8f5820b4041979c469611cc1b52 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-21T01:35:30Z |
| publishDate | 2019-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-60f1e8f5820b4041979c469611cc1b522022-12-21T19:20:16ZengElsevierJournal of Integrative Agriculture2095-31192019-02-01182417427Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thalianaYa-bin ZHANG0Wei TANG1Li-huan WANG2Ya-wen HU3Xian-wen LIU4Yong-sheng LIU5Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, P.R.China; ZHANG Ya-bin, Mobile: +86-18227630935Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, P.R.China; School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, P.R.China; Correspondence TANG Wei, Mobile: +86-13339017320Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, P.R.ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, P.R.ChinaSchool of Food Science and Engineering, Hefei University of Technology, Hefei 230009, P.R.ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, P.R.China; School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, P.R.China; Correspondence LIU Yong-sheng, Mobile: +86-18709832886Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses, such as drought, heat, cold, water logging and phytopathogens. Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement. Here we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog (AcMYB3R) whose expression was induced by drought, salinity and cold stress. In vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene KNOLLE. The Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt stress. The expressions of stress-responsive genes such as RD29A, RD29B, COR15A and RD22 were prominently up-regulated by ectopic expression of AcMYB3R. Our study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production.http://www.sciencedirect.com/science/article/pii/S2095311918621276kiwifruitR1R2R3-MYBdroughtabiotic stress |
| spellingShingle | Ya-bin ZHANG Wei TANG Li-huan WANG Ya-wen HU Xian-wen LIU Yong-sheng LIU Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana Journal of Integrative Agriculture kiwifruit R1R2R3-MYB drought abiotic stress |
| title | Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana |
| title_full | Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana |
| title_fullStr | Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana |
| title_full_unstemmed | Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana |
| title_short | Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana |
| title_sort | kiwifruit actinidia chinensis r1r2r3 myb transcription factor acmyb3r enhances drought and salinity tolerance in arabidopsis thaliana |
| topic | kiwifruit R1R2R3-MYB drought abiotic stress |
| url | http://www.sciencedirect.com/science/article/pii/S2095311918621276 |
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