Effects of hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitro
The aim of this work was to investigate the effect of the commercial formulation hemazin SC 500, an herbicide containing terbuthylazine as the active compound, on the isoenzyme patterns and activities of Cu-Zn superoxide dismutase (SOD1) and catalase (CAT), as well as on the glutathione S-transferas...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2019-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391800115D.pdf |
| _version_ | 1818645383049379840 |
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| author | Davidović-Plavšić Biljana Lukić Nataša Nikolić-Kokić Aleksandra Kukavica Biljana |
| author_facet | Davidović-Plavšić Biljana Lukić Nataša Nikolić-Kokić Aleksandra Kukavica Biljana |
| author_sort | Davidović-Plavšić Biljana |
| collection | DOAJ |
| description | The aim of this work was to investigate the effect of the commercial formulation hemazin SC 500, an herbicide containing terbuthylazine as the active compound, on the isoenzyme patterns and activities of Cu-Zn superoxide dismutase (SOD1) and catalase (CAT), as well as on the glutathione S-transferase (GST) activity, in human erythrocytes in vitro. The human erythrocytes were treated with hemazin SC 500 over a broad range of terbuthylazine concentrations (37 nmol L-1– –37 mol L-1) for 1 and 3 h at a temperature of 37°C. Native electrophoresis of the control and treated samples revealed two SOD1 and one CAT isoform. Treatment did not affect the SOD1 and CAT isoenzyme profile, but induced a change in their activities. Terbuthylazine at lower concentration induced a significant increase of the total SOD1 activity and decreased the GST activity in samples incubated for 1 and 3 h. On the other hand, the highest increase in the CAT activity was observed for the sample treated for 1 h with a higher concentration of terbuthylazine. Hemazin SC 500 containing terbuthylazine induces changes in the erythrocyte antioxidative system whereby the response of individual enzymatic antioxidants depends on the concentration of the pesticide and the incubation time. |
| first_indexed | 2024-12-17T00:29:52Z |
| format | Article |
| id | doaj.art-60f39280586a4a00bb1dcfeb5df9a1e4 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-17T00:29:52Z |
| publishDate | 2019-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-60f39280586a4a00bb1dcfeb5df9a1e42022-12-21T22:10:21ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212019-01-0184545546510.2298/JSC181011115D0352-51391800115DEffects of hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitroDavidović-Plavšić Biljana0Lukić Nataša1Nikolić-Kokić Aleksandra2Kukavica Biljana3University of Banja Luka, Faculty of Natural Sciences and Mathematics, Banja Luka, Bosnia and HerzegovinaFaculty of Sciences, Novi SadInstitute for Biological Research ”Siniša Stanković“, BelgradeUniversity of Banja Luka, Faculty of Natural Sciences and Mathematics, Banja Luka, Bosnia and HerzegovinaThe aim of this work was to investigate the effect of the commercial formulation hemazin SC 500, an herbicide containing terbuthylazine as the active compound, on the isoenzyme patterns and activities of Cu-Zn superoxide dismutase (SOD1) and catalase (CAT), as well as on the glutathione S-transferase (GST) activity, in human erythrocytes in vitro. The human erythrocytes were treated with hemazin SC 500 over a broad range of terbuthylazine concentrations (37 nmol L-1– –37 mol L-1) for 1 and 3 h at a temperature of 37°C. Native electrophoresis of the control and treated samples revealed two SOD1 and one CAT isoform. Treatment did not affect the SOD1 and CAT isoenzyme profile, but induced a change in their activities. Terbuthylazine at lower concentration induced a significant increase of the total SOD1 activity and decreased the GST activity in samples incubated for 1 and 3 h. On the other hand, the highest increase in the CAT activity was observed for the sample treated for 1 h with a higher concentration of terbuthylazine. Hemazin SC 500 containing terbuthylazine induces changes in the erythrocyte antioxidative system whereby the response of individual enzymatic antioxidants depends on the concentration of the pesticide and the incubation time.http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391800115D.pdfherbicidehemolysateSOD1catalaseglutathione-S-transferase |
| spellingShingle | Davidović-Plavšić Biljana Lukić Nataša Nikolić-Kokić Aleksandra Kukavica Biljana Effects of hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitro Journal of the Serbian Chemical Society herbicide hemolysate SOD1 catalase glutathione-S-transferase |
| title | Effects of hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitro |
| title_full | Effects of hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitro |
| title_fullStr | Effects of hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitro |
| title_full_unstemmed | Effects of hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitro |
| title_short | Effects of hemazin SC 500 (terbuthylazine) on antioxidative enzymes in human erythrocytes in vitro |
| title_sort | effects of hemazin sc 500 terbuthylazine on antioxidative enzymes in human erythrocytes in vitro |
| topic | herbicide hemolysate SOD1 catalase glutathione-S-transferase |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391800115D.pdf |
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