Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet Hamsters
Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease caused by oxidative stress, inflammation and lipid deposition within liver cells, and is subsequently contributing to cardiovascular diseases such as atherosclerosis. Deep sea water (DSW) is characterized by its clearance and abund...
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MDPI AG
2022-01-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/1/82 |
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| author | Chia-Chun Wu Yu-Hsuan Cheng Kuo-Hsin Chen Chiang-Ting Chien |
| author_facet | Chia-Chun Wu Yu-Hsuan Cheng Kuo-Hsin Chen Chiang-Ting Chien |
| author_sort | Chia-Chun Wu |
| collection | DOAJ |
| description | Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease caused by oxidative stress, inflammation and lipid deposition within liver cells, and is subsequently contributing to cardiovascular diseases such as atherosclerosis. Deep sea water (DSW) is characterized by its clearance and abundant nutrients with antioxidant and anti-inflammatory activity to confer therapeutic potential. We aimed to explore the therapeutic capability of our prepared multi-filtration DSW-dissolved organic matter (DSW-DOM) on high-fat diet-induced hyperlipidemia and endothelial dysfunction in hamsters. A high-fat/high-cholesterol diet led to increased oxidative stress, including blood reactive oxygen species (ROS), plasma malondialdehyde (MDA) and hepatic CYP2E1 expression; an increased hyperlipidemic profile and SREBP 1-mediated fatty liver; promoted NFκB p65-mediated hepatic inflammation; triggered PARP-mediated hepatic apoptosis; and enhanced endothelial intercellular adhesion molecule-1 (ICAM-1) and von Willebrand factor (VWF)-mediated atherosclerosis associated with the depressed hepatic antioxidant Paraoxonase 1 (PON1) expression. The DSW-DOM-enriched 1295 fraction, with strong H<sub>2</sub>O<sub>2</sub> scavenging activity, efficiently reduced several oxidative stress parameters, the lipid profile, inflammation, and apoptosis, possibly through the PON1-mediated antioxidant capability. Furthermore, DSW-DOM treatment significantly decreased the endothelial ICAM-1 and VWF expression, subsequently leading to the elongation of time to occlusion of FeCl<sub>3</sub>-induced arterial thrombosis and to the inhibition of FeCl<sub>3</sub>-induced fluorescent platelet adhesion to mesentery arterioles in the high-fat diet. Based on the above results, our data suggest that DSW-DOM intake via antioxidant defense mechanisms confers protective effects against high-fat diet-enhanced, oxidative stress-mediated hyperlipidemia, and endothelial dysfunction evoked atherosclerosis by downregulating oxidative injury, lipogenesis, inflammation and apoptosis. |
| first_indexed | 2024-03-10T01:07:22Z |
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| language | English |
| last_indexed | 2024-03-10T01:07:22Z |
| publishDate | 2022-01-01 |
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| series | Life |
| spelling | doaj.art-60f4b1308ec24787b2a05463fc1e9b2c2023-11-23T14:24:46ZengMDPI AGLife2075-17292022-01-011218210.3390/life12010082Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet HamstersChia-Chun Wu0Yu-Hsuan Cheng1Kuo-Hsin Chen2Chiang-Ting Chien3Department of Life Science, School of Life Science, College of Science, National Taiwan Normal University, Taipei 11677, TaiwanDepartment of Life Science, School of Life Science, College of Science, National Taiwan Normal University, Taipei 11677, TaiwanDepartment of Surgery, Division of General Surgery, Far-Eastern Memorial Hospital, New Taipei City 22056, TaiwanDepartment of Life Science, School of Life Science, College of Science, National Taiwan Normal University, Taipei 11677, TaiwanNon-alcoholic fatty liver disease (NAFLD) is a chronic liver disease caused by oxidative stress, inflammation and lipid deposition within liver cells, and is subsequently contributing to cardiovascular diseases such as atherosclerosis. Deep sea water (DSW) is characterized by its clearance and abundant nutrients with antioxidant and anti-inflammatory activity to confer therapeutic potential. We aimed to explore the therapeutic capability of our prepared multi-filtration DSW-dissolved organic matter (DSW-DOM) on high-fat diet-induced hyperlipidemia and endothelial dysfunction in hamsters. A high-fat/high-cholesterol diet led to increased oxidative stress, including blood reactive oxygen species (ROS), plasma malondialdehyde (MDA) and hepatic CYP2E1 expression; an increased hyperlipidemic profile and SREBP 1-mediated fatty liver; promoted NFκB p65-mediated hepatic inflammation; triggered PARP-mediated hepatic apoptosis; and enhanced endothelial intercellular adhesion molecule-1 (ICAM-1) and von Willebrand factor (VWF)-mediated atherosclerosis associated with the depressed hepatic antioxidant Paraoxonase 1 (PON1) expression. The DSW-DOM-enriched 1295 fraction, with strong H<sub>2</sub>O<sub>2</sub> scavenging activity, efficiently reduced several oxidative stress parameters, the lipid profile, inflammation, and apoptosis, possibly through the PON1-mediated antioxidant capability. Furthermore, DSW-DOM treatment significantly decreased the endothelial ICAM-1 and VWF expression, subsequently leading to the elongation of time to occlusion of FeCl<sub>3</sub>-induced arterial thrombosis and to the inhibition of FeCl<sub>3</sub>-induced fluorescent platelet adhesion to mesentery arterioles in the high-fat diet. Based on the above results, our data suggest that DSW-DOM intake via antioxidant defense mechanisms confers protective effects against high-fat diet-enhanced, oxidative stress-mediated hyperlipidemia, and endothelial dysfunction evoked atherosclerosis by downregulating oxidative injury, lipogenesis, inflammation and apoptosis.https://www.mdpi.com/2075-1729/12/1/82hyperlipidemiadeep sea water-dissolved organic matternon-alcoholic fatty liver diseaseatherosclerosisapoptosisplatelet adhesion |
| spellingShingle | Chia-Chun Wu Yu-Hsuan Cheng Kuo-Hsin Chen Chiang-Ting Chien Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet Hamsters Life hyperlipidemia deep sea water-dissolved organic matter non-alcoholic fatty liver disease atherosclerosis apoptosis platelet adhesion |
| title | Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet Hamsters |
| title_full | Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet Hamsters |
| title_fullStr | Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet Hamsters |
| title_full_unstemmed | Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet Hamsters |
| title_short | Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet Hamsters |
| title_sort | deep sea water dissolved organic matter intake improves hyperlipidemia and inhibits thrombus formation and vascular inflammation in high fat diet hamsters |
| topic | hyperlipidemia deep sea water-dissolved organic matter non-alcoholic fatty liver disease atherosclerosis apoptosis platelet adhesion |
| url | https://www.mdpi.com/2075-1729/12/1/82 |
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