Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extrac...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2015-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025&lng=en&tlng=en |
| _version_ | 1818571116776521728 |
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| author | Qiangcheng Zeng Yong Guo Yongming Liu Ruixin Li Xinchang Zhang Lu Liu Yang Wang Xizheng Zhang Xianqiong Zou |
| author_facet | Qiangcheng Zeng Yong Guo Yongming Liu Ruixin Li Xinchang Zhang Lu Liu Yang Wang Xizheng Zhang Xianqiong Zou |
| author_sort | Qiangcheng Zeng |
| collection | DOAJ |
| description | BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-β1 siRNA or integrin-β5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µε) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-β1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-β5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation. CONCLUSION: Integrin-β1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-β5 is not involved in the osteoblasts response to the tensile strain. |
| first_indexed | 2024-12-14T13:51:19Z |
| format | Article |
| id | doaj.art-60fac1e03c6a43dcad13466256fb9fa2 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-12-14T13:51:19Z |
| publishDate | 2015-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-60fac1e03c6a43dcad13466256fb9fa22022-12-21T22:59:03ZengBMCBiological Research0716-97602015-01-014801810.1186/s40659-015-0014-yS0716-97602015000100025Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strainQiangcheng Zeng0Yong Guo1Yongming Liu2Ruixin Li3Xinchang Zhang4Lu Liu5Yang Wang6Xizheng Zhang7Xianqiong Zou8Dezhou UniversityGuilin Medical UniversityGuilin Medical UniversityAcademy of Military Medical SciencesAcademy of Military Medical SciencesLogistics College of Chinese People's Armed Police ForcesGuilin Medical UniversityAcademy of Military Medical SciencesGuilin Medical UniversityBACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-β1 siRNA or integrin-β5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µε) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-β1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-β5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation. CONCLUSION: Integrin-β1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-β5 is not involved in the osteoblasts response to the tensile strain.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025&lng=en&tlng=enTensile strainOsteoblastExtracellular matrixIntegrinsiRNA |
| spellingShingle | Qiangcheng Zeng Yong Guo Yongming Liu Ruixin Li Xinchang Zhang Lu Liu Yang Wang Xizheng Zhang Xianqiong Zou Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain Biological Research Tensile strain Osteoblast Extracellular matrix Integrin siRNA |
| title | Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain |
| title_full | Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain |
| title_fullStr | Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain |
| title_full_unstemmed | Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain |
| title_short | Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain |
| title_sort | integrin β1 not integrin β5 mediates osteoblastic differentiation and ecm formation promoted by mechanical tensile strain |
| topic | Tensile strain Osteoblast Extracellular matrix Integrin siRNA |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025&lng=en&tlng=en |
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