Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480
Objective To investigate the effect of miR-200c-5p on malignant proliferation of human colorectal cancer cell line SW480. Methods Human colorectal cancer cell line SW480 was transfected with miR-200c-5p mimic/inhibitor and negative control agent respectively. The expression of miR-200c-5p was dete...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-2-241.pdf |
| _version_ | 1797366348684197888 |
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| author | WANG Fangfang, CAO Huiyuan, WANG Jing, WANG Ning, HUANG Guoliang |
| author_facet | WANG Fangfang, CAO Huiyuan, WANG Jing, WANG Ning, HUANG Guoliang |
| author_sort | WANG Fangfang, CAO Huiyuan, WANG Jing, WANG Ning, HUANG Guoliang |
| collection | DOAJ |
| description | Objective To investigate the effect of miR-200c-5p on malignant proliferation of human colorectal cancer cell line SW480. Methods Human colorectal cancer cell line SW480 was transfected with miR-200c-5p mimic/inhibitor and negative control agent respectively. The expression of miR-200c-5p was detected by qPCR, and the effects of miR-200c-5p on the proliferation activity and clonogenesis ability of colorectal cancer cells were detected by CCK-8 and foci formation experiment. Bioinformatics methods were used to analyze the differential target genes and related pathways of miR-200c-5p in human colorectal cancer. Results Transient transfection with miR-200c-5p mimic/inhibitor succeeded in high/low expression of miR-200c-5p (P＜0.05). The proliferation of cells with high expression of miR-200c-5p was significantly inhibited as compare to that of controls(P＜0.05). The clone-formation rate of miR-200c-5p cells with high expression was significantly lower than that of untransfected cells, while the clone-formation rate of miR-200c-5p cells with low expression was the opposite (P＜0.05). CXCL10, the target gene of miR-200c-5p, was significantly up-regulated in colorectal cancer, while C2orf72, ZC3H12C and DST were significantly down-regulated. The most significant KEGG pathways were “melanoma”, “microRNAs in cancer” and “bladder cancer”. Conclusions miR-200c-5p inhibits the proliferation and clonal formation of human colorectal cancer cell line SW480, which is related to the occurrence and progression of colorectal cancer. |
| first_indexed | 2024-03-08T17:02:58Z |
| format | Article |
| id | doaj.art-60fbde1684a04ae2a05e27778e83c4f0 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:02:58Z |
| publishDate | 2023-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-60fbde1684a04ae2a05e27778e83c4f02024-01-04T07:27:29ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-02-0143224124510.16352/j.issn.1001-6325.2023.02.241Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480WANG Fangfang, CAO Huiyuan, WANG Jing, WANG Ning, HUANG Guoliang0Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, the First Dongguan Affiliated Hospital, Key Laboratory for Epigenetics of Dongguan City, China-America Cancer Research Institute, Guangdong Medical University, Dongguan 523808, ChinaObjective To investigate the effect of miR-200c-5p on malignant proliferation of human colorectal cancer cell line SW480. Methods Human colorectal cancer cell line SW480 was transfected with miR-200c-5p mimic/inhibitor and negative control agent respectively. The expression of miR-200c-5p was detected by qPCR, and the effects of miR-200c-5p on the proliferation activity and clonogenesis ability of colorectal cancer cells were detected by CCK-8 and foci formation experiment. Bioinformatics methods were used to analyze the differential target genes and related pathways of miR-200c-5p in human colorectal cancer. Results Transient transfection with miR-200c-5p mimic/inhibitor succeeded in high/low expression of miR-200c-5p (P＜0.05). The proliferation of cells with high expression of miR-200c-5p was significantly inhibited as compare to that of controls(P＜0.05). The clone-formation rate of miR-200c-5p cells with high expression was significantly lower than that of untransfected cells, while the clone-formation rate of miR-200c-5p cells with low expression was the opposite (P＜0.05). CXCL10, the target gene of miR-200c-5p, was significantly up-regulated in colorectal cancer, while C2orf72, ZC3H12C and DST were significantly down-regulated. The most significant KEGG pathways were “melanoma”, “microRNAs in cancer” and “bladder cancer”. Conclusions miR-200c-5p inhibits the proliferation and clonal formation of human colorectal cancer cell line SW480, which is related to the occurrence and progression of colorectal cancer.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-2-241.pdfcolorectal cancer|mir-200c-5p|cell proliferation |
| spellingShingle | WANG Fangfang, CAO Huiyuan, WANG Jing, WANG Ning, HUANG Guoliang Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480 Jichu yixue yu linchuang colorectal cancer|mir-200c-5p|cell proliferation |
| title | Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480 |
| title_full | Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480 |
| title_fullStr | Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480 |
| title_full_unstemmed | Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480 |
| title_short | Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480 |
| title_sort | up regulation of mir 200c 5p inhibits proliferation of human colorectal cancer cell line sw480 |
| topic | colorectal cancer|mir-200c-5p|cell proliferation |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-2-241.pdf |
| work_keys_str_mv | AT wangfangfangcaohuiyuanwangjingwangninghuangguoliang upregulationofmir200c5pinhibitsproliferationofhumancolorectalcancercelllinesw480 |