Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering
Abstract Electrospun scaffolds play important roles in the fields of regenerative medicine and vascular tissue engineering. The aim of the research described here was to develop a vascular scaffold that mimics the structural and functional properties of natural vascular scaffolding. The mechanical p...
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-54638-0 |
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| author | Lei Yang Xu Wang Man Xiong Xinfang Liu Sidong Luo Jinxian Luo Yeyang Wang |
| author_facet | Lei Yang Xu Wang Man Xiong Xinfang Liu Sidong Luo Jinxian Luo Yeyang Wang |
| author_sort | Lei Yang |
| collection | DOAJ |
| description | Abstract Electrospun scaffolds play important roles in the fields of regenerative medicine and vascular tissue engineering. The aim of the research described here was to develop a vascular scaffold that mimics the structural and functional properties of natural vascular scaffolding. The mechanical properties of artificial vascular tissue represent a key issue for successful transplantation in small diameter engineering blood vessels. We blended silk fibroin (SF) and fibrin to fabricate a composite scaffold using electrospinning to overcome the shortcomings of fibrin with respect to its mechanical properties. Subsequently, we then carefully investigated the morphological, mechanical properties, hydrophilicity, hemocompatibility, degradation, cytocompatibility and biocompatibility of the SF/fibrin (0:100), SF/fibrin (15:85), SF/fibrin (25:75), and SF/fibrin (35:65) scaffolds. Based on these in vitro results, we implanted SF/fibrin (25:75) vascular scaffold subcutaneously and analyzed its in vivo degradation and histocompatibility. The fiber structure of the SF/fibrin hybrid scaffold was smooth and uniform, and its fiber diameters were relatively small. Compared with the fibrin scaffold, the SF/fibrin scaffold clearly displayed increased mechanical strength, but the hydrophilicity weakened correspondingly. All of the SF/fibrin scaffolds showed excellent blood compatibility and appropriate biodegradation rates. The SF/fibrin (25:75) scaffold increased the proliferation and adhesion of MSCs. The results of animal experiments confirmed that the degradation of the SF/fibrin (25:75) scaffold was faster than that of the SF scaffold and effectively promoted tissue regeneration and cell infiltration. All in all, the SF/fibrin (25:75) electrospun scaffold displayed balanced and controllable biomechanical properties, degradability, and good cell compatibility. Thus, this scaffold proved to be an ideal candidate material for artificial blood vessels. |
| first_indexed | 2024-03-07T15:03:19Z |
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| id | doaj.art-60fcc44571b940ee9203f85d89fb7a8f |
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| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:03:19Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-60fcc44571b940ee9203f85d89fb7a8f2024-03-05T19:00:20ZengNature PortfolioScientific Reports2045-23222024-02-0114111710.1038/s41598-024-54638-0Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineeringLei Yang0Xu Wang1Man Xiong2Xinfang Liu3Sidong Luo4Jinxian Luo5Yeyang Wang6Department of Surgical Base, Guangdong Second Provincial General HospitalBiomedical College, Guangdong University of TechnologySchool of Nursing, Guangzhou University of Chinese MedicineOrthopaedic Center, Guangdong Second Provincial General HospitalOrthopaedic Center, Guangdong Second Provincial General HospitalDepartment of Thyroid and Mammary Surgery, Guangdong Second Provincial General HospitalOrthopaedic Center, Guangdong Second Provincial General HospitalAbstract Electrospun scaffolds play important roles in the fields of regenerative medicine and vascular tissue engineering. The aim of the research described here was to develop a vascular scaffold that mimics the structural and functional properties of natural vascular scaffolding. The mechanical properties of artificial vascular tissue represent a key issue for successful transplantation in small diameter engineering blood vessels. We blended silk fibroin (SF) and fibrin to fabricate a composite scaffold using electrospinning to overcome the shortcomings of fibrin with respect to its mechanical properties. Subsequently, we then carefully investigated the morphological, mechanical properties, hydrophilicity, hemocompatibility, degradation, cytocompatibility and biocompatibility of the SF/fibrin (0:100), SF/fibrin (15:85), SF/fibrin (25:75), and SF/fibrin (35:65) scaffolds. Based on these in vitro results, we implanted SF/fibrin (25:75) vascular scaffold subcutaneously and analyzed its in vivo degradation and histocompatibility. The fiber structure of the SF/fibrin hybrid scaffold was smooth and uniform, and its fiber diameters were relatively small. Compared with the fibrin scaffold, the SF/fibrin scaffold clearly displayed increased mechanical strength, but the hydrophilicity weakened correspondingly. All of the SF/fibrin scaffolds showed excellent blood compatibility and appropriate biodegradation rates. The SF/fibrin (25:75) scaffold increased the proliferation and adhesion of MSCs. The results of animal experiments confirmed that the degradation of the SF/fibrin (25:75) scaffold was faster than that of the SF scaffold and effectively promoted tissue regeneration and cell infiltration. All in all, the SF/fibrin (25:75) electrospun scaffold displayed balanced and controllable biomechanical properties, degradability, and good cell compatibility. Thus, this scaffold proved to be an ideal candidate material for artificial blood vessels.https://doi.org/10.1038/s41598-024-54638-0SF/fibrin scaffoldMechanical strengthElectrospunVascularTissue engineering |
| spellingShingle | Lei Yang Xu Wang Man Xiong Xinfang Liu Sidong Luo Jinxian Luo Yeyang Wang Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering Scientific Reports SF/fibrin scaffold Mechanical strength Electrospun Vascular Tissue engineering |
| title | Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering |
| title_full | Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering |
| title_fullStr | Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering |
| title_full_unstemmed | Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering |
| title_short | Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering |
| title_sort | electrospun silk fibroin fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering |
| topic | SF/fibrin scaffold Mechanical strength Electrospun Vascular Tissue engineering |
| url | https://doi.org/10.1038/s41598-024-54638-0 |
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