Analysis of the Expression of Angioarchitecture-related Factors in Patients with Cerebral Arteriovenous Malformation
Background: Cerebral arteriovenous malformation (cAVM) is a type of vascular malformation associated with vascular remodeling, hemodynamic imbalance, and inflammation. We detected four angioarchitecture-related cytokines to make a better understanding of the potential aberrant signaling in the patho...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2017-01-01
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| Series: | Chinese Medical Journal |
| Subjects: | |
| Online Access: | http://www.cmj.org/article.asp?issn=0366-6999;year=2017;volume=130;issue=20;spage=2465;epage=2472;aulast=Chen |
| _version_ | 1818408185253330944 |
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| author | Guang-Zhong Chen Yu Ke Kun Qin Meng-Qi Dong Shao-Jian Zeng Xiao-Feng Lin Sheng-Quan Zhan Kai Tang Chao Peng Xiao-Wen Ding Dong Zhou |
| author_facet | Guang-Zhong Chen Yu Ke Kun Qin Meng-Qi Dong Shao-Jian Zeng Xiao-Feng Lin Sheng-Quan Zhan Kai Tang Chao Peng Xiao-Wen Ding Dong Zhou |
| author_sort | Guang-Zhong Chen |
| collection | DOAJ |
| description | Background: Cerebral arteriovenous malformation (cAVM) is a type of vascular malformation associated with vascular remodeling, hemodynamic imbalance, and inflammation. We detected four angioarchitecture-related cytokines to make a better understanding of the potential aberrant signaling in the pathogenesis of cAVM and found useful proteins in predicting the risk of cerebral hemorrhage.
Methods: Immunohistochemical analysis was conducted on specimens from twenty patients with cAVM diagnosed via magnetic resonance imaging and digital subtraction angiography and twenty primary epilepsy controls using antibodies against vascular endothelial growth factor receptor-2 (VEGFR-2), matrix metalloproteinase-9 (MMP-9), vascular cell adhesion molecule (VCAM-1), and endothelial nitric oxide synthase (eNOS). Western blotting and real-time fluorescent quantitative polymerase chain reaction (PCR) were performed to determine protein and mRNA expression levels. Student's t-test was used for statistical analysis.
Results: VEGFR-2, MMP-9, VCAM-1, and eNOS expression levels increased in patients with cAVM compared with those in normal cerebral vascular tissue, as determined by immunohistochemical analysis. In addition, Western blotting and real-time PCR showed that the protein and mRNA expression levels of VEGFR-2, MMP-9, VCAM-1, and eNOS were higher in the cAVM group than in the control group, all the differences mentioned were statistically significant (P < 0.05).
Conclusions: VEGFR-2, MMP-9, VCAM-1, and eNOS are upregulated in patients with cAVM and might play important roles in angiogenesis, vascular remodeling, and migration in patients with cAVM. MMP-9, VEGFR-2, VCAM-1, and eNOS might be potential excellent group proteins in predicting the risk of cerebral hemorrhage at arteriovenous malformation. |
| first_indexed | 2024-12-14T09:39:43Z |
| format | Article |
| id | doaj.art-60fd5b027b4b4c8296982b6c00d186fb |
| institution | Directory Open Access Journal |
| issn | 0366-6999 |
| language | English |
| last_indexed | 2024-12-14T09:39:43Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-60fd5b027b4b4c8296982b6c00d186fb2022-12-21T23:07:50ZengWolters KluwerChinese Medical Journal0366-69992017-01-01130202465247210.4103/0366-6999.216413Analysis of the Expression of Angioarchitecture-related Factors in Patients with Cerebral Arteriovenous MalformationGuang-Zhong ChenYu KeKun QinMeng-Qi DongShao-Jian ZengXiao-Feng LinSheng-Quan ZhanKai TangChao PengXiao-Wen DingDong ZhouBackground: Cerebral arteriovenous malformation (cAVM) is a type of vascular malformation associated with vascular remodeling, hemodynamic imbalance, and inflammation. We detected four angioarchitecture-related cytokines to make a better understanding of the potential aberrant signaling in the pathogenesis of cAVM and found useful proteins in predicting the risk of cerebral hemorrhage. Methods: Immunohistochemical analysis was conducted on specimens from twenty patients with cAVM diagnosed via magnetic resonance imaging and digital subtraction angiography and twenty primary epilepsy controls using antibodies against vascular endothelial growth factor receptor-2 (VEGFR-2), matrix metalloproteinase-9 (MMP-9), vascular cell adhesion molecule (VCAM-1), and endothelial nitric oxide synthase (eNOS). Western blotting and real-time fluorescent quantitative polymerase chain reaction (PCR) were performed to determine protein and mRNA expression levels. Student's t-test was used for statistical analysis. Results: VEGFR-2, MMP-9, VCAM-1, and eNOS expression levels increased in patients with cAVM compared with those in normal cerebral vascular tissue, as determined by immunohistochemical analysis. In addition, Western blotting and real-time PCR showed that the protein and mRNA expression levels of VEGFR-2, MMP-9, VCAM-1, and eNOS were higher in the cAVM group than in the control group, all the differences mentioned were statistically significant (P < 0.05). Conclusions: VEGFR-2, MMP-9, VCAM-1, and eNOS are upregulated in patients with cAVM and might play important roles in angiogenesis, vascular remodeling, and migration in patients with cAVM. MMP-9, VEGFR-2, VCAM-1, and eNOS might be potential excellent group proteins in predicting the risk of cerebral hemorrhage at arteriovenous malformation.http://www.cmj.org/article.asp?issn=0366-6999;year=2017;volume=130;issue=20;spage=2465;epage=2472;aulast=ChenAngioarchitecture-related Factors; Cerebral Arteriovenous Malformation; Endothelial Nitric Oxide Synthase; Matrix Metalloproteinase-9; Vascular Cell Adhesion Molecule-1; Vascular Endothelial Growth Factor Receptor-2 |
| spellingShingle | Guang-Zhong Chen Yu Ke Kun Qin Meng-Qi Dong Shao-Jian Zeng Xiao-Feng Lin Sheng-Quan Zhan Kai Tang Chao Peng Xiao-Wen Ding Dong Zhou Analysis of the Expression of Angioarchitecture-related Factors in Patients with Cerebral Arteriovenous Malformation Chinese Medical Journal Angioarchitecture-related Factors; Cerebral Arteriovenous Malformation; Endothelial Nitric Oxide Synthase; Matrix Metalloproteinase-9; Vascular Cell Adhesion Molecule-1; Vascular Endothelial Growth Factor Receptor-2 |
| title | Analysis of the Expression of Angioarchitecture-related Factors in Patients with Cerebral Arteriovenous Malformation |
| title_full | Analysis of the Expression of Angioarchitecture-related Factors in Patients with Cerebral Arteriovenous Malformation |
| title_fullStr | Analysis of the Expression of Angioarchitecture-related Factors in Patients with Cerebral Arteriovenous Malformation |
| title_full_unstemmed | Analysis of the Expression of Angioarchitecture-related Factors in Patients with Cerebral Arteriovenous Malformation |
| title_short | Analysis of the Expression of Angioarchitecture-related Factors in Patients with Cerebral Arteriovenous Malformation |
| title_sort | analysis of the expression of angioarchitecture related factors in patients with cerebral arteriovenous malformation |
| topic | Angioarchitecture-related Factors; Cerebral Arteriovenous Malformation; Endothelial Nitric Oxide Synthase; Matrix Metalloproteinase-9; Vascular Cell Adhesion Molecule-1; Vascular Endothelial Growth Factor Receptor-2 |
| url | http://www.cmj.org/article.asp?issn=0366-6999;year=2017;volume=130;issue=20;spage=2465;epage=2472;aulast=Chen |
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