MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity
Local populations of <i>Apis mellifera</i> are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial <i>tRNA<...
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MDPI AG
2021-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/9/767 |
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| author | Marija Tanasković Pavle Erić Aleksandra Patenković Katarina Erić Milica Mihajlović Vanja Tanasić Ljubiša Stanisavljević Slobodan Davidović |
| author_facet | Marija Tanasković Pavle Erić Aleksandra Patenković Katarina Erić Milica Mihajlović Vanja Tanasić Ljubiša Stanisavljević Slobodan Davidović |
| author_sort | Marija Tanasković |
| collection | DOAJ |
| description | Local populations of <i>Apis mellifera</i> are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial <i>tRNA<sup>leu</sup>-cox2</i> intergenic region of 241 worker bees from 46 apiaries at eight localities. Nine haplotypes were observed in our samples, with C2d being the most common and widespread. To evaluate genetic diversity patterns, we compared our data with 1696 sequences from the NCBI GenBank from neighbouring countries and Serbia. All 32 detected haplotypes belonged to the Southeast Europe lineage C, with two newly described haplotypes from our sample. The most frequent haplotype was C2d, followed by C2c and C1a. To distinguish <i>A. m. carnica</i> from <i>A. m. macedonica</i>, both previously reported in Serbia, PCR-RFLP analysis on the <i>COI</i> gene segment of mtDNA was used, and the result showed only the presence of <i>A.m. carnica</i> subspecies. An MDS plot constructed on pairwise <i>F<sub>ST</sub></i> values showed significant geographical stratification. Our samples are grouped together, but distant from the Serbian dataset from the GenBank. This, with the absence of <i>A. m. macedonica</i> subspecies from its historic range of distribution in southern Serbia, indicates that honey bee populations are changing rapidly due to the anthropogenic influence. |
| first_indexed | 2024-03-10T07:34:48Z |
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| language | English |
| last_indexed | 2024-03-10T07:34:48Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-60fddfb744994354add7e1da14b6ed522023-11-22T13:36:11ZengMDPI AGInsects2075-44502021-08-0112976710.3390/insects12090767MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees DiversityMarija Tanasković0Pavle Erić1Aleksandra Patenković2Katarina Erić3Milica Mihajlović4Vanja Tanasić5Ljubiša Stanisavljević6Slobodan Davidović7Department of Genetics of Populations and Ecogenotoxicology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Genetics of Populations and Ecogenotoxicology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Genetics of Populations and Ecogenotoxicology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Genetics of Populations and Ecogenotoxicology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaCenter for Forensic and Applied Molecular Genetics, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaCenter for Forensic and Applied Molecular Genetics, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaCenter for Bee Research, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaDepartment of Genetics of Populations and Ecogenotoxicology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaLocal populations of <i>Apis mellifera</i> are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial <i>tRNA<sup>leu</sup>-cox2</i> intergenic region of 241 worker bees from 46 apiaries at eight localities. Nine haplotypes were observed in our samples, with C2d being the most common and widespread. To evaluate genetic diversity patterns, we compared our data with 1696 sequences from the NCBI GenBank from neighbouring countries and Serbia. All 32 detected haplotypes belonged to the Southeast Europe lineage C, with two newly described haplotypes from our sample. The most frequent haplotype was C2d, followed by C2c and C1a. To distinguish <i>A. m. carnica</i> from <i>A. m. macedonica</i>, both previously reported in Serbia, PCR-RFLP analysis on the <i>COI</i> gene segment of mtDNA was used, and the result showed only the presence of <i>A.m. carnica</i> subspecies. An MDS plot constructed on pairwise <i>F<sub>ST</sub></i> values showed significant geographical stratification. Our samples are grouped together, but distant from the Serbian dataset from the GenBank. This, with the absence of <i>A. m. macedonica</i> subspecies from its historic range of distribution in southern Serbia, indicates that honey bee populations are changing rapidly due to the anthropogenic influence.https://www.mdpi.com/2075-4450/12/9/767honey beemtDNApopulation geneticshaplotype diversitysubspecies |
| spellingShingle | Marija Tanasković Pavle Erić Aleksandra Patenković Katarina Erić Milica Mihajlović Vanja Tanasić Ljubiša Stanisavljević Slobodan Davidović MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity Insects honey bee mtDNA population genetics haplotype diversity subspecies |
| title | MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity |
| title_full | MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity |
| title_fullStr | MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity |
| title_full_unstemmed | MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity |
| title_short | MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity |
| title_sort | mtdna analysis indicates human induced temporal changes of serbian honey bees diversity |
| topic | honey bee mtDNA population genetics haplotype diversity subspecies |
| url | https://www.mdpi.com/2075-4450/12/9/767 |
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