Bread crust extract protects rats’ vascular grafts from in vitro ischemia/reperfusion injury
Ischemia/reperfusion (IR) injury affects harvested vascular grafts during coronary artery bypass surgery. We hypothesized that bread crust extract (BCE) protects vascular grafts from IR injury. Thoracic aortic rings from Wistar rats were mounted on organ bath chambers (control) or subjected to in vi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622002572 |
| _version_ | 1828526190674051072 |
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| author | Sevil Korkmaz-Icöz Marcel Schwär Sivakkanan Loganathan Kristin Wächter Adrian-Iustin Georgevici Patricia Kraft Tobias Mayer Andreas Simm Matthias Karck Gábor Szabó |
| author_facet | Sevil Korkmaz-Icöz Marcel Schwär Sivakkanan Loganathan Kristin Wächter Adrian-Iustin Georgevici Patricia Kraft Tobias Mayer Andreas Simm Matthias Karck Gábor Szabó |
| author_sort | Sevil Korkmaz-Icöz |
| collection | DOAJ |
| description | Ischemia/reperfusion (IR) injury affects harvested vascular grafts during coronary artery bypass surgery. We hypothesized that bread crust extract (BCE) protects vascular grafts from IR injury. Thoracic aortic rings from Wistar rats were mounted on organ bath chambers (control) or subjected to in vitro IR injury either in saline (IR) or saline-supplemented with BCE (IR + BCE). Impaired maximum endothelium-dependent vasorelaxation to acetylcholine (control 73 ± 2 % vs IR 40 ± 2 % vs IR + BCE 63 ± 3 %, p < 0.05), altered maximal phenylephrine-induced contractile responses and high K+-induced depolarization in the IR-group compared to controls were improved by BCE. Computer-assisted artificial intelligence methods, applied on gene expression data, showed that increased Ccl2 expression (>-7.23) with a decrease in the anti-oxidant gene Gclc (≤-2.947) and Stab1 (≤-6.144) levels were reflecting IR-group, whereas increased Ccl2 expression (>-7.23) with an increased Gclc (>-2.947) level were indicatory for IR + BCE-rings. Furthermore, BCE lowered nitrotyrosine and poly(ADP-ribose)-polymerase-1 immunoreactivity. BCE protects vascular grafts from in vitro IR injury. |
| first_indexed | 2024-12-11T21:19:02Z |
| format | Article |
| id | doaj.art-60ff250b77b14f2eb68ed89276f14d71 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-11T21:19:02Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-60ff250b77b14f2eb68ed89276f14d712022-12-22T00:50:30ZengElsevierJournal of Functional Foods1756-46462022-08-0195105187Bread crust extract protects rats’ vascular grafts from in vitro ischemia/reperfusion injurySevil Korkmaz-Icöz0Marcel Schwär1Sivakkanan Loganathan2Kristin Wächter3Adrian-Iustin Georgevici4Patricia Kraft5Tobias Mayer6Andreas Simm7Matthias Karck8Gábor Szabó9Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany; Department of Cardiac Surgery, University Hospital Halle (Saale), 06120 Halle, Germany; Corresponding author at: Laboratory of Cardiac Surgery, Department of Cardiac Surgery, University Hospital Heidelberg, INF 326, 69120 Heidelberg, Germany.Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany; Department of Cardiac Surgery, University Hospital Halle (Saale), 06120 Halle, GermanyDepartment of Cardiac Surgery, University Hospital Halle (Saale), 06120 Halle, GermanyDepartment of Cardiac Surgery, University Hospital Halle (Saale), 06120 Halle, Germany; Department of Anesthesiology, St. Josef Hospital, Ruhr-University Bochum, 44791 Bochum, GermanyDepartment of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Cardiac Surgery, University Hospital Halle (Saale), 06120 Halle, GermanyDepartment of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany; Department of Cardiac Surgery, University Hospital Halle (Saale), 06120 Halle, GermanyIschemia/reperfusion (IR) injury affects harvested vascular grafts during coronary artery bypass surgery. We hypothesized that bread crust extract (BCE) protects vascular grafts from IR injury. Thoracic aortic rings from Wistar rats were mounted on organ bath chambers (control) or subjected to in vitro IR injury either in saline (IR) or saline-supplemented with BCE (IR + BCE). Impaired maximum endothelium-dependent vasorelaxation to acetylcholine (control 73 ± 2 % vs IR 40 ± 2 % vs IR + BCE 63 ± 3 %, p < 0.05), altered maximal phenylephrine-induced contractile responses and high K+-induced depolarization in the IR-group compared to controls were improved by BCE. Computer-assisted artificial intelligence methods, applied on gene expression data, showed that increased Ccl2 expression (>-7.23) with a decrease in the anti-oxidant gene Gclc (≤-2.947) and Stab1 (≤-6.144) levels were reflecting IR-group, whereas increased Ccl2 expression (>-7.23) with an increased Gclc (>-2.947) level were indicatory for IR + BCE-rings. Furthermore, BCE lowered nitrotyrosine and poly(ADP-ribose)-polymerase-1 immunoreactivity. BCE protects vascular grafts from in vitro IR injury.http://www.sciencedirect.com/science/article/pii/S1756464622002572Ischemia/reperfusionEndothelial dysfunctionBread crust extractVascular function |
| spellingShingle | Sevil Korkmaz-Icöz Marcel Schwär Sivakkanan Loganathan Kristin Wächter Adrian-Iustin Georgevici Patricia Kraft Tobias Mayer Andreas Simm Matthias Karck Gábor Szabó Bread crust extract protects rats’ vascular grafts from in vitro ischemia/reperfusion injury Journal of Functional Foods Ischemia/reperfusion Endothelial dysfunction Bread crust extract Vascular function |
| title | Bread crust extract protects rats’ vascular grafts from in vitro ischemia/reperfusion injury |
| title_full | Bread crust extract protects rats’ vascular grafts from in vitro ischemia/reperfusion injury |
| title_fullStr | Bread crust extract protects rats’ vascular grafts from in vitro ischemia/reperfusion injury |
| title_full_unstemmed | Bread crust extract protects rats’ vascular grafts from in vitro ischemia/reperfusion injury |
| title_short | Bread crust extract protects rats’ vascular grafts from in vitro ischemia/reperfusion injury |
| title_sort | bread crust extract protects rats vascular grafts from in vitro ischemia reperfusion injury |
| topic | Ischemia/reperfusion Endothelial dysfunction Bread crust extract Vascular function |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622002572 |
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