Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brain
Summary: Stem cell therapy shows promise for multiple disorders; however, the molecular crosstalk between grafted cells and host tissue is largely unknown. Here, we take a step toward addressing this question. Using translating ribosome affinity purification (TRAP) with sequencing tools, we simultan...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723003649 |
| _version_ | 1797848239867691008 |
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| author | Ricardo L. Azevedo-Pereira Nathan C. Manley Chen Dong Yue Zhang Alex G. Lee Yulia Zatulovskaia Varun Gupta Jennifer Vu Summer Han Jack E. Berry Tonya M. Bliss Gary K. Steinberg |
| author_facet | Ricardo L. Azevedo-Pereira Nathan C. Manley Chen Dong Yue Zhang Alex G. Lee Yulia Zatulovskaia Varun Gupta Jennifer Vu Summer Han Jack E. Berry Tonya M. Bliss Gary K. Steinberg |
| author_sort | Ricardo L. Azevedo-Pereira |
| collection | DOAJ |
| description | Summary: Stem cell therapy shows promise for multiple disorders; however, the molecular crosstalk between grafted cells and host tissue is largely unknown. Here, we take a step toward addressing this question. Using translating ribosome affinity purification (TRAP) with sequencing tools, we simultaneously decode the transcriptomes of graft and host for human neural stem cells (hNSCs) transplanted into the stroke-injured rat brain. Employing pathway analysis tools, we investigate the interactions between the two transcriptomes to predict molecular pathways linking host and graft genes; as proof of concept, we predict host-secreted factors that signal to the graft and the downstream molecular cascades they trigger in the graft. We identify a potential host-graft crosstalk pathway where BMP6 from the stroke-injured brain induces graft secretion of noggin, a known brain repair factor. Decoding the molecular interplay between graft and host is a critical step toward deciphering the molecular mechanisms of stem cell action. |
| first_indexed | 2024-04-09T18:24:17Z |
| format | Article |
| id | doaj.art-60fffa56c0664d14a22e17445291e752 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-09T18:24:17Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-60fffa56c0664d14a22e17445291e7522023-04-12T04:11:42ZengElsevierCell Reports2211-12472023-04-01424112353Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brainRicardo L. Azevedo-Pereira0Nathan C. Manley1Chen Dong2Yue Zhang3Alex G. Lee4Yulia Zatulovskaia5Varun Gupta6Jennifer Vu7Summer Han8Jack E. Berry9Tonya M. Bliss10Gary K. Steinberg11Department of Neurosurgery, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USAStanford Genetics Bioinformatics Service Center, Stanford University, Stanford, CA 94305, USADivision of Hematology and Oncology, Department of Pediatrics, University of California, San Francisco, CA 94143, USADepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USADepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA; Corresponding authorDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA; Corresponding authorSummary: Stem cell therapy shows promise for multiple disorders; however, the molecular crosstalk between grafted cells and host tissue is largely unknown. Here, we take a step toward addressing this question. Using translating ribosome affinity purification (TRAP) with sequencing tools, we simultaneously decode the transcriptomes of graft and host for human neural stem cells (hNSCs) transplanted into the stroke-injured rat brain. Employing pathway analysis tools, we investigate the interactions between the two transcriptomes to predict molecular pathways linking host and graft genes; as proof of concept, we predict host-secreted factors that signal to the graft and the downstream molecular cascades they trigger in the graft. We identify a potential host-graft crosstalk pathway where BMP6 from the stroke-injured brain induces graft secretion of noggin, a known brain repair factor. Decoding the molecular interplay between graft and host is a critical step toward deciphering the molecular mechanisms of stem cell action.http://www.sciencedirect.com/science/article/pii/S2211124723003649CP: NeuroscienceCP: Stem cell research |
| spellingShingle | Ricardo L. Azevedo-Pereira Nathan C. Manley Chen Dong Yue Zhang Alex G. Lee Yulia Zatulovskaia Varun Gupta Jennifer Vu Summer Han Jack E. Berry Tonya M. Bliss Gary K. Steinberg Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brain Cell Reports CP: Neuroscience CP: Stem cell research |
| title | Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brain |
| title_full | Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brain |
| title_fullStr | Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brain |
| title_full_unstemmed | Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brain |
| title_short | Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brain |
| title_sort | decoding the molecular crosstalk between grafted stem cells and the stroke injured brain |
| topic | CP: Neuroscience CP: Stem cell research |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723003649 |
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