Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism
<p>Abstract</p> <p>Background</p> <p>Elucidating metabolic network structures and functions in multicellular organisms is an emerging goal of functional genomics. We describe the co-expression network of three core metabolic processes in the genetic model plant <it&g...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-07-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/8/76 |
| _version_ | 1811285305062326272 |
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| author | Nikolau Basil J Ransom Nick Peng Jianling Mentzen Wieslawa I Wurtele Eve |
| author_facet | Nikolau Basil J Ransom Nick Peng Jianling Mentzen Wieslawa I Wurtele Eve |
| author_sort | Nikolau Basil J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Elucidating metabolic network structures and functions in multicellular organisms is an emerging goal of functional genomics. We describe the co-expression network of three core metabolic processes in the genetic model plant <it>Arabidopsis thaliana</it>: fatty acid biosynthesis, starch metabolism and amino acid (leucine) catabolism.</p> <p>Results</p> <p>These co-expression networks form modules populated by genes coding for enzymes that represent the reactions generally considered to define each pathway. However, the modules also incorporate a wider set of genes that encode transporters, cofactor biosynthetic enzymes, precursor-producing enzymes, and regulatory molecules. We tested experimentally the hypothesis that one of the genes tightly co-expressed with starch metabolism module, a putative kinase AtPERK10, will have a role in this process. Indeed, knockout lines of AtPERK10 have an altered starch accumulation. In addition, the co-expression data define a novel hierarchical transcript-level structure associated with catabolism, in which genes performing smaller, more specific tasks appear to be recruited into higher-order modules with a broader catabolic function.</p> <p>Conclusion</p> <p>Each of these core metabolic pathways is structured as a module of co-expressed transcripts that co-accumulate over a wide range of environmental and genetic perturbations and developmental stages, and represent an expanded set of macromolecules associated with the common task of supporting the functionality of each metabolic pathway. As experimentally demonstrated, co-expression analysis can provide a rich approach towards understanding gene function.</p> |
| first_indexed | 2024-04-13T02:42:00Z |
| format | Article |
| id | doaj.art-610162e7de674ea89b4a7983e4fdacb7 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T02:42:00Z |
| publishDate | 2008-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-610162e7de674ea89b4a7983e4fdacb72022-12-22T03:06:09ZengBMCBMC Plant Biology1471-22292008-07-01817610.1186/1471-2229-8-76Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolismNikolau Basil JRansom NickPeng JianlingMentzen Wieslawa IWurtele Eve<p>Abstract</p> <p>Background</p> <p>Elucidating metabolic network structures and functions in multicellular organisms is an emerging goal of functional genomics. We describe the co-expression network of three core metabolic processes in the genetic model plant <it>Arabidopsis thaliana</it>: fatty acid biosynthesis, starch metabolism and amino acid (leucine) catabolism.</p> <p>Results</p> <p>These co-expression networks form modules populated by genes coding for enzymes that represent the reactions generally considered to define each pathway. However, the modules also incorporate a wider set of genes that encode transporters, cofactor biosynthetic enzymes, precursor-producing enzymes, and regulatory molecules. We tested experimentally the hypothesis that one of the genes tightly co-expressed with starch metabolism module, a putative kinase AtPERK10, will have a role in this process. Indeed, knockout lines of AtPERK10 have an altered starch accumulation. In addition, the co-expression data define a novel hierarchical transcript-level structure associated with catabolism, in which genes performing smaller, more specific tasks appear to be recruited into higher-order modules with a broader catabolic function.</p> <p>Conclusion</p> <p>Each of these core metabolic pathways is structured as a module of co-expressed transcripts that co-accumulate over a wide range of environmental and genetic perturbations and developmental stages, and represent an expanded set of macromolecules associated with the common task of supporting the functionality of each metabolic pathway. As experimentally demonstrated, co-expression analysis can provide a rich approach towards understanding gene function.</p>http://www.biomedcentral.com/1471-2229/8/76 |
| spellingShingle | Nikolau Basil J Ransom Nick Peng Jianling Mentzen Wieslawa I Wurtele Eve Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism BMC Plant Biology |
| title | Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism |
| title_full | Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism |
| title_fullStr | Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism |
| title_full_unstemmed | Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism |
| title_short | Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism |
| title_sort | articulation of three core metabolic processes in arabidopsis fatty acid biosynthesis leucine catabolism and starch metabolism |
| url | http://www.biomedcentral.com/1471-2229/8/76 |
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