Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes
Quinolone resistance in members of the <em>Enterobacteriaceae</em> family is mostly due to mutations in the quinolone resistance-determining regions of topoisomerase genes. CTX-M-22 is a member of the CTX-M family which can reduce extended-spectrum β-lactamase (ESBL) prod...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Via Medica
2012-04-01
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| Series: | Folia Histochemica et Cytobiologica |
| Subjects: | |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/18698 |
| _version_ | 1818268156262612992 |
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| author | Lianhua Yu Jin Zhang Yong Liang Yongxiao Qi Haibao Wang Dongguo Wang |
| author_facet | Lianhua Yu Jin Zhang Yong Liang Yongxiao Qi Haibao Wang Dongguo Wang |
| author_sort | Lianhua Yu |
| collection | DOAJ |
| description | Quinolone resistance in members of the <em>Enterobacteriaceae</em> family is mostly due to mutations in the quinolone resistance-determining regions of topoisomerase genes. CTX-M-22 is a member of the CTX-M family which can reduce extended-spectrum β-lactamase (ESBL) production and modulate antibiotic resistance, resulting in low ceftazidime minimum inhibitory concentrations (MICs). There are four different genes in <em>Klebsiella pneumoniae</em> (KP4707) including qnrB32 (novel qnr allele gene, HQ704413), aac(6’)-Ib-cr (novel aac(6’)-Ib allele gene, HQ680690), gyrA (novel gyrA allele gene, HQ680691) and CTX-M-22 gene. Five point amino acid mutations Arn(N)27 → Leu(L), Val(V)129 → Ala(A), Iie(I)142 → Met(M), Gly(G)188 → Arg(R), Val(V)212 → Iie(I) were observed in the qnr32 gene when compared to qnrB1. Of all qnrB alleles, a novel variant of the qnrB32 gene, with qnrB31, had the highest amino acid homology. Three point amino acid mutations including Trp(W)105 → Arg(R), Asp(D)182 → Tyr(Y) and Val(V)201 → Asp(D) were observed in aac(6’)-Ib-cr gene, when compared to GenBank number AF479774. New variants of qnr32, aac(6’)-Ib-cr, gyrA and CTX-M-22 or other genotype determinants continuously appear in different genomic sites and also outside the <em>Enterobacteriaceae</em> family. |
| first_indexed | 2024-12-12T20:34:00Z |
| format | Article |
| id | doaj.art-6101977b57a2494cb2e0941af6d3f8d5 |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-12-12T20:34:00Z |
| publishDate | 2012-04-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-6101977b57a2494cb2e0941af6d3f8d52022-12-22T00:12:58ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312012-04-01501687410.5603/18698Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genesLianhua YuJin ZhangYong LiangYongxiao QiHaibao WangDongguo WangQuinolone resistance in members of the <em>Enterobacteriaceae</em> family is mostly due to mutations in the quinolone resistance-determining regions of topoisomerase genes. CTX-M-22 is a member of the CTX-M family which can reduce extended-spectrum β-lactamase (ESBL) production and modulate antibiotic resistance, resulting in low ceftazidime minimum inhibitory concentrations (MICs). There are four different genes in <em>Klebsiella pneumoniae</em> (KP4707) including qnrB32 (novel qnr allele gene, HQ704413), aac(6’)-Ib-cr (novel aac(6’)-Ib allele gene, HQ680690), gyrA (novel gyrA allele gene, HQ680691) and CTX-M-22 gene. Five point amino acid mutations Arn(N)27 → Leu(L), Val(V)129 → Ala(A), Iie(I)142 → Met(M), Gly(G)188 → Arg(R), Val(V)212 → Iie(I) were observed in the qnr32 gene when compared to qnrB1. Of all qnrB alleles, a novel variant of the qnrB32 gene, with qnrB31, had the highest amino acid homology. Three point amino acid mutations including Trp(W)105 → Arg(R), Asp(D)182 → Tyr(Y) and Val(V)201 → Asp(D) were observed in aac(6’)-Ib-cr gene, when compared to GenBank number AF479774. New variants of qnr32, aac(6’)-Ib-cr, gyrA and CTX-M-22 or other genotype determinants continuously appear in different genomic sites and also outside the <em>Enterobacteriaceae</em> family.http://czasopisma.viamedica.pl/fhc/article/view/18698Klebsiella pneumoniaeqnrB32aac(6’)-Ib-crgyrACTX-M-22 |
| spellingShingle | Lianhua Yu Jin Zhang Yong Liang Yongxiao Qi Haibao Wang Dongguo Wang Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes Folia Histochemica et Cytobiologica Klebsiella pneumoniae qnrB32 aac(6’)-Ib-cr gyrA CTX-M-22 |
| title | Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes |
| title_full | Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes |
| title_fullStr | Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes |
| title_full_unstemmed | Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes |
| title_short | Characteristics of Klebsiella pneumoniae harboring QnrB32, Aac(6’)-Ib-cr, GyrA and CTX-M-22 genes |
| title_sort | characteristics of klebsiella pneumoniae harboring qnrb32 aac 6 ib cr gyra and ctx m 22 genes |
| topic | Klebsiella pneumoniae qnrB32 aac(6’)-Ib-cr gyrA CTX-M-22 |
| url | http://czasopisma.viamedica.pl/fhc/article/view/18698 |
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