First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes)
ABSTRACT Gymnorhamphichthys britskii is a Neotropical electric fish of family Rhamphichthyidae described from the Paraná-Paraguay system. This study reports the first karyotypic description of G. britskii collected from the upper Paraná river basin, which presented 2n=38 chromosomes, karyotype compo...
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Sociedade Brasileira de Ictiologia
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Series: | Neotropical Ichthyology |
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author | Carlos Alexandre Fernandes Allan Kardec Moreira de Aguiar Leonardo Marcel Paiz Lucas Baumgärtner Diovani Piscor Vladimir Pavan Margarido |
author_facet | Carlos Alexandre Fernandes Allan Kardec Moreira de Aguiar Leonardo Marcel Paiz Lucas Baumgärtner Diovani Piscor Vladimir Pavan Margarido |
author_sort | Carlos Alexandre Fernandes |
collection | DOAJ |
description | ABSTRACT Gymnorhamphichthys britskii is a Neotropical electric fish of family Rhamphichthyidae described from the Paraná-Paraguay system. This study reports the first karyotypic description of G. britskii collected from the upper Paraná river basin, which presented 2n=38 chromosomes, karyotype composed of 14 metacentric, 8 submetacentric, 2 subtelocentric and 14 acrocentric chromosomes, and fundamental number as 62 for both sexes. Heteromorphic sex chromosomes were absent. A single pair of nucleolar organizing regions (NORs) was detected in the submetacentric chromosome pair number 9 by silver staining and confirmed by the 18S rDNA probe. The 5S rDNA was located in a single chromosome pair. Heterochromatic regions were clearly observed in the short arms of the NOR-bearing chromosome pair and in the telomeric positions of most acrocentric chromosomes. Besides the present data are valuable to help in understanding karyotypic evolution in Rhamphichthyidae, data from NORs confirmed the tendency of this family in presenting simple NORs sites, similar to the other Gymnotiformes clades. Yet, the presence of a large heterochromatic block in the NOR-bearing chromosome can be used as cytogenetic markers for G. britskii, and that centric fusions appear to be an important mechanism in the karyotype evolution and differentiation among Gymnotiformes species. |
first_indexed | 2024-12-10T10:05:38Z |
format | Article |
id | doaj.art-6101f91a22ff429b89c04fb462fa2e50 |
institution | Directory Open Access Journal |
issn | 1982-0224 |
language | English |
last_indexed | 2024-12-10T10:05:38Z |
publisher | Sociedade Brasileira de Ictiologia |
record_format | Article |
series | Neotropical Ichthyology |
spelling | doaj.art-6101f91a22ff429b89c04fb462fa2e502022-12-22T01:53:16ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-022417310.1590/1982-0224-20190069S1679-62252019000300205First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes)Carlos Alexandre FernandesAllan Kardec Moreira de AguiarLeonardo Marcel PaizLucas BaumgärtnerDiovani PiscorVladimir Pavan MargaridoABSTRACT Gymnorhamphichthys britskii is a Neotropical electric fish of family Rhamphichthyidae described from the Paraná-Paraguay system. This study reports the first karyotypic description of G. britskii collected from the upper Paraná river basin, which presented 2n=38 chromosomes, karyotype composed of 14 metacentric, 8 submetacentric, 2 subtelocentric and 14 acrocentric chromosomes, and fundamental number as 62 for both sexes. Heteromorphic sex chromosomes were absent. A single pair of nucleolar organizing regions (NORs) was detected in the submetacentric chromosome pair number 9 by silver staining and confirmed by the 18S rDNA probe. The 5S rDNA was located in a single chromosome pair. Heterochromatic regions were clearly observed in the short arms of the NOR-bearing chromosome pair and in the telomeric positions of most acrocentric chromosomes. Besides the present data are valuable to help in understanding karyotypic evolution in Rhamphichthyidae, data from NORs confirmed the tendency of this family in presenting simple NORs sites, similar to the other Gymnotiformes clades. Yet, the presence of a large heterochromatic block in the NOR-bearing chromosome can be used as cytogenetic markers for G. britskii, and that centric fusions appear to be an important mechanism in the karyotype evolution and differentiation among Gymnotiformes species.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000300205&lng=en&tlng=enAg-NORsC-bandingFish cytogeneticsKaryotype evolutionRepetitive sequences |
spellingShingle | Carlos Alexandre Fernandes Allan Kardec Moreira de Aguiar Leonardo Marcel Paiz Lucas Baumgärtner Diovani Piscor Vladimir Pavan Margarido First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes) Neotropical Ichthyology Ag-NORs C-banding Fish cytogenetics Karyotype evolution Repetitive sequences |
title | First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes) |
title_full | First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes) |
title_fullStr | First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes) |
title_full_unstemmed | First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes) |
title_short | First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes) |
title_sort | first chromosomal analysis of gymnorhamphichthys britskii the remarkable lowest diploid value within the family rhamphichthyidae gymnotiformes |
topic | Ag-NORs C-banding Fish cytogenetics Karyotype evolution Repetitive sequences |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000300205&lng=en&tlng=en |
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