The value of cuproptosis-related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanoma
Background: Skin cutaneous melanoma (SKCM) is one of the most common cutaneous malignancies, which incidence is increasing. Cuproptosis is a new type of programming cell death recently reported, which may affect the progression of SKCM.Method: The mRNA expression data of melanoma were obtained from...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1129544/full |
| _version_ | 1797845601556103168 |
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| author | Yuming Sun Shaorong Lei Xiangyue Luo Chufeng Jiang Zhexuan Li |
| author_facet | Yuming Sun Shaorong Lei Xiangyue Luo Chufeng Jiang Zhexuan Li |
| author_sort | Yuming Sun |
| collection | DOAJ |
| description | Background: Skin cutaneous melanoma (SKCM) is one of the most common cutaneous malignancies, which incidence is increasing. Cuproptosis is a new type of programming cell death recently reported, which may affect the progression of SKCM.Method: The mRNA expression data of melanoma were obtained from the Gene Expression Omnibus and the Cancer Genome Atlas databases. We constructed a prognostic model according to the cuproptosis-related differential genes in SKCM. Finally, real-time quantitative PCR was performed to verify the expression of cuproptosis-related differential genes in patients with different stages of cutaneous melanoma.Results: We detected 767 cuproptosis-related differential genes based on 19 cuproptosis-related genes, and screened out 7 differential genes to construct a prognostic model, which including three high-risk differential genes (SNAI2, RAP1GAP, BCHE), and four low-risk differential genes (JSRP1, HAPLN3, HHEX, ERAP2). Kaplan-Meier analysis indicated that SKCM patients with low-risk differential genes signals had better prognosis. The Encyclopedia of Genomes results manifested that cuproptosis-related differential genes are not only involved in T cell receptor signaling channel, natural killer cell mediated cytotoxicity, but also chemokine signaling pathway and B cell receptor signaling pathway. In our risk scoring model, the receiver operating characteristic (ROC) values of the three-time nodes are 0.669 (1-year), 0.669 (3-year) and 0.685 (5-year), respectively. Moreover, the tumor burden mutational and immunology function, cell stemness characteristics and drug sensitivity have significant differences between low-risk group and high-risk group. The mRNA level of SNAI2, RAP1GAP and BCHE in stage Ⅲ+Ⅳ SKCM patients was significantly higher than that in stage Ⅰ+Ⅱ patients, while the level of JSRP1, HAPLN3, HHEX and ERAP2 in stage Ⅰ+Ⅱ SKCM patients was more remarkable higher than that in stage Ⅲ+Ⅳ SKCM patients.Conclusion: In summary, we suggest that cuproptosis can not only regulate the tumor immune microenvironment but also affect the prognosis of SKCM patients, and may offer a basic theory for SKCM patients survival studies and clinical decision-making with potentially therapeutic drugs. |
| first_indexed | 2024-04-09T17:41:40Z |
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| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-09T17:41:40Z |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-610483207d7c46b1a9d80f4d9f9c37aa2023-04-17T04:59:14ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-04-011410.3389/fphar.2023.11295441129544The value of cuproptosis-related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanomaYuming SunShaorong LeiXiangyue LuoChufeng JiangZhexuan LiBackground: Skin cutaneous melanoma (SKCM) is one of the most common cutaneous malignancies, which incidence is increasing. Cuproptosis is a new type of programming cell death recently reported, which may affect the progression of SKCM.Method: The mRNA expression data of melanoma were obtained from the Gene Expression Omnibus and the Cancer Genome Atlas databases. We constructed a prognostic model according to the cuproptosis-related differential genes in SKCM. Finally, real-time quantitative PCR was performed to verify the expression of cuproptosis-related differential genes in patients with different stages of cutaneous melanoma.Results: We detected 767 cuproptosis-related differential genes based on 19 cuproptosis-related genes, and screened out 7 differential genes to construct a prognostic model, which including three high-risk differential genes (SNAI2, RAP1GAP, BCHE), and four low-risk differential genes (JSRP1, HAPLN3, HHEX, ERAP2). Kaplan-Meier analysis indicated that SKCM patients with low-risk differential genes signals had better prognosis. The Encyclopedia of Genomes results manifested that cuproptosis-related differential genes are not only involved in T cell receptor signaling channel, natural killer cell mediated cytotoxicity, but also chemokine signaling pathway and B cell receptor signaling pathway. In our risk scoring model, the receiver operating characteristic (ROC) values of the three-time nodes are 0.669 (1-year), 0.669 (3-year) and 0.685 (5-year), respectively. Moreover, the tumor burden mutational and immunology function, cell stemness characteristics and drug sensitivity have significant differences between low-risk group and high-risk group. The mRNA level of SNAI2, RAP1GAP and BCHE in stage Ⅲ+Ⅳ SKCM patients was significantly higher than that in stage Ⅰ+Ⅱ patients, while the level of JSRP1, HAPLN3, HHEX and ERAP2 in stage Ⅰ+Ⅱ SKCM patients was more remarkable higher than that in stage Ⅲ+Ⅳ SKCM patients.Conclusion: In summary, we suggest that cuproptosis can not only regulate the tumor immune microenvironment but also affect the prognosis of SKCM patients, and may offer a basic theory for SKCM patients survival studies and clinical decision-making with potentially therapeutic drugs.https://www.frontiersin.org/articles/10.3389/fphar.2023.1129544/fullcuproptosisprognostic modelskin cutaneous melanomabioinformaticstumor mutational burden (TMB) |
| spellingShingle | Yuming Sun Shaorong Lei Xiangyue Luo Chufeng Jiang Zhexuan Li The value of cuproptosis-related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanoma Frontiers in Pharmacology cuproptosis prognostic model skin cutaneous melanoma bioinformatics tumor mutational burden (TMB) |
| title | The value of cuproptosis-related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanoma |
| title_full | The value of cuproptosis-related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanoma |
| title_fullStr | The value of cuproptosis-related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanoma |
| title_full_unstemmed | The value of cuproptosis-related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanoma |
| title_short | The value of cuproptosis-related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanoma |
| title_sort | value of cuproptosis related differential genes in guiding prognosis and immune status in patients with skin cutaneous melanoma |
| topic | cuproptosis prognostic model skin cutaneous melanoma bioinformatics tumor mutational burden (TMB) |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1129544/full |
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