Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering
The current study aimed to evaluate the characteristics and the effects of degradation on the structural properties of Poly(lactic-co-glycolic acid) (PLGA)- and polycaprolactone (PCL)-based nanofibrous scaffolds. Six scaffolds were prepared by electrospinning, three with PCL 15% (<i>w</i>...
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| author | Morteza Bazgir Wei Zhang Ximu Zhang Jacobo Elies Morvarid Saeinasab Phil Coates Mansour Youseffi Farshid Sefat |
| author_facet | Morteza Bazgir Wei Zhang Ximu Zhang Jacobo Elies Morvarid Saeinasab Phil Coates Mansour Youseffi Farshid Sefat |
| author_sort | Morteza Bazgir |
| collection | DOAJ |
| description | The current study aimed to evaluate the characteristics and the effects of degradation on the structural properties of Poly(lactic-co-glycolic acid) (PLGA)- and polycaprolactone (PCL)-based nanofibrous scaffolds. Six scaffolds were prepared by electrospinning, three with PCL 15% (<i>w</i>/<i>v</i>) and three with PLGA 10% (<i>w</i>/<i>v</i>), with electrospinning processing times of 30, 60 and 90 min. Both types of scaffolds displayed more robust mechanical properties with increased spinning times. The tensile strength of both scaffolds with 90-min electrospun membranes did not show a significant difference in their strengths, as the PCL and PLGA scaffolds measured at 1.492 MPa ± 0.378 SD and 1.764 MPa ± 0.7982 SD, respectively. All membranes were shown to be hydrophobic under a wettability test. A degradation behaviour study was performed by immersing all scaffolds in phosphate-buffered saline (PBS) solution at room temperature for 12 weeks and for 4 weeks at 37 °C. The effects of degradation were monitored by taking each sample out of the PBS solution every week, and the structural changes were investigated under a scanning electron microscope (SEM). The PCL and PLGA scaffolds showed excellent fibre structure with adequate degradation, and the fibre diameter, measured over time, showed slight increase in size. Therefore, as an example of fibre water intake and progressive degradation, the scaffold’s percentage weight loss increased each week, further supporting the porous membrane’s degradability. The pore size and the porosity percentage of all scaffolds decreased substantially over the degradation period. The conclusion drawn from this experiment is that PCL and PLGA hold great promise for tissue engineering and regenerative medicine applications. |
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| language | English |
| last_indexed | 2024-03-10T08:08:45Z |
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| spelling | doaj.art-610514f41ba84a099a0c454eca02086e2023-11-22T10:51:24ZengMDPI AGMaterials1996-19442021-08-011417477310.3390/ma14174773Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue EngineeringMorteza Bazgir0Wei Zhang1Ximu Zhang2Jacobo Elies3Morvarid Saeinasab4Phil Coates5Mansour Youseffi6Farshid Sefat7Department of Biomedical and Electronics Engineering, School of Engineering, University of Bradford, Bradford BD7 1DP, UKState Key Laboratory of Polymer Materials Engineering, Polymer Research Institute, Sichuan University, Chengdu 610065, ChinaChongqing Key Laboratory of Oral Disease and Biomedical Sciences and Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University, Chongqing 401174, ChinaFaculty of Life Sciences, School of Pharmacy and Medical Sciences, University of Bradford, Bradford BD7 1DP, UKDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad 9177948974, IranInterdisciplinary Research Centre in Polymer Science and Technology (Polymer IRC), University of Bradford, Bradford BD7 1DP, UKDepartment of Biomedical and Electronics Engineering, School of Engineering, University of Bradford, Bradford BD7 1DP, UKDepartment of Biomedical and Electronics Engineering, School of Engineering, University of Bradford, Bradford BD7 1DP, UKThe current study aimed to evaluate the characteristics and the effects of degradation on the structural properties of Poly(lactic-co-glycolic acid) (PLGA)- and polycaprolactone (PCL)-based nanofibrous scaffolds. Six scaffolds were prepared by electrospinning, three with PCL 15% (<i>w</i>/<i>v</i>) and three with PLGA 10% (<i>w</i>/<i>v</i>), with electrospinning processing times of 30, 60 and 90 min. Both types of scaffolds displayed more robust mechanical properties with increased spinning times. The tensile strength of both scaffolds with 90-min electrospun membranes did not show a significant difference in their strengths, as the PCL and PLGA scaffolds measured at 1.492 MPa ± 0.378 SD and 1.764 MPa ± 0.7982 SD, respectively. All membranes were shown to be hydrophobic under a wettability test. A degradation behaviour study was performed by immersing all scaffolds in phosphate-buffered saline (PBS) solution at room temperature for 12 weeks and for 4 weeks at 37 °C. The effects of degradation were monitored by taking each sample out of the PBS solution every week, and the structural changes were investigated under a scanning electron microscope (SEM). The PCL and PLGA scaffolds showed excellent fibre structure with adequate degradation, and the fibre diameter, measured over time, showed slight increase in size. Therefore, as an example of fibre water intake and progressive degradation, the scaffold’s percentage weight loss increased each week, further supporting the porous membrane’s degradability. The pore size and the porosity percentage of all scaffolds decreased substantially over the degradation period. The conclusion drawn from this experiment is that PCL and PLGA hold great promise for tissue engineering and regenerative medicine applications.https://www.mdpi.com/1996-1944/14/17/4773electrospinningpolycaprolactone (PCL)Poly(lactic-co-glycolic acid) (PLGA)tissue engineeringporous biodegradable membranedegradation |
| spellingShingle | Morteza Bazgir Wei Zhang Ximu Zhang Jacobo Elies Morvarid Saeinasab Phil Coates Mansour Youseffi Farshid Sefat Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering Materials electrospinning polycaprolactone (PCL) Poly(lactic-co-glycolic acid) (PLGA) tissue engineering porous biodegradable membrane degradation |
| title | Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering |
| title_full | Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering |
| title_fullStr | Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering |
| title_full_unstemmed | Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering |
| title_short | Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering |
| title_sort | degradation and characterisation of electrospun polycaprolactone pcl and poly lactic co glycolic acid plga scaffolds for vascular tissue engineering |
| topic | electrospinning polycaprolactone (PCL) Poly(lactic-co-glycolic acid) (PLGA) tissue engineering porous biodegradable membrane degradation |
| url | https://www.mdpi.com/1996-1944/14/17/4773 |
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