Identification and characterization of mycosporine-like amino acid in the cyanobacterium Scytonema sp. HKAR-16 by HPLC, Raman spectroscopy, and ESI-MS
The incidence of ultraviolet radiation has increased on the Earth's surface due to depletion of the stratospheric ozone layer which has several detrimental effects on living organisms including cyanobacteria. Mycosporine-like amino acids (MAAs) are eco-friendly natural sun screening biomolecu...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2022-06-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://sjst.psu.ac.th/journal/44-3/31.pdf |
| _version_ | 1797848972410224640 |
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| author | Sonal Mishra Niharika Sahu Neha Kumari Richa Rajeshwar P. Sinha |
| author_facet | Sonal Mishra Niharika Sahu Neha Kumari Richa Rajeshwar P. Sinha |
| author_sort | Sonal Mishra |
| collection | DOAJ |
| description | The incidence of ultraviolet radiation has increased on the Earth's surface due to depletion of the stratospheric ozone
layer which has several detrimental effects on living organisms including cyanobacteria. Mycosporine-like amino acids (MAAs)
are eco-friendly natural sun screening biomolecules having prominent photoprotective potentials. Our study provides the
identification and characterization of one of these MAAs from the cyanobacterium Scytonema sp. strain HKAR-16. The
identification and characterization of MAA palythine (λmax = 320 nm; m/z = 245.1) from this strain was performed using
ultraviolet-visible spectrophotometer, high-performance liquid chromatography (HPLC) associated with photodiode array (PDA)
detector and electrospray ionization-mass spectrometry (ESI-MS) analyses. Further characterization was performed by Raman
spectroscopy. To the best of our knowledge, this is the first report regarding the presence of MAA palythine in Scytonema sp.
HKAR-16. Our results indicate that the cyanobacterium can protect itself by synthesizing MAA palythine in response to injurious
UV radiation. This naturally occurring photoprotective compound may have the potential to replace synthetic photoprotectants
that can be used by the humans.
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| first_indexed | 2024-04-09T18:36:16Z |
| format | Article |
| id | doaj.art-610cbb7094784086b4451dbed616bae1 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-09T18:36:16Z |
| publishDate | 2022-06-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-610cbb7094784086b4451dbed616bae12023-04-11T08:44:38ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952022-06-0144383684010.14456/sjst-psu.2022.111Identification and characterization of mycosporine-like amino acid in the cyanobacterium Scytonema sp. HKAR-16 by HPLC, Raman spectroscopy, and ESI-MSSonal Mishra0Niharika Sahu1Neha Kumari2Richa3Rajeshwar P. Sinha4Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005 IndiaLaboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005 IndiaLaboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005 IndiaLaboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005 IndiaLaboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005 IndiaThe incidence of ultraviolet radiation has increased on the Earth's surface due to depletion of the stratospheric ozone layer which has several detrimental effects on living organisms including cyanobacteria. Mycosporine-like amino acids (MAAs) are eco-friendly natural sun screening biomolecules having prominent photoprotective potentials. Our study provides the identification and characterization of one of these MAAs from the cyanobacterium Scytonema sp. strain HKAR-16. The identification and characterization of MAA palythine (λmax = 320 nm; m/z = 245.1) from this strain was performed using ultraviolet-visible spectrophotometer, high-performance liquid chromatography (HPLC) associated with photodiode array (PDA) detector and electrospray ionization-mass spectrometry (ESI-MS) analyses. Further characterization was performed by Raman spectroscopy. To the best of our knowledge, this is the first report regarding the presence of MAA palythine in Scytonema sp. HKAR-16. Our results indicate that the cyanobacterium can protect itself by synthesizing MAA palythine in response to injurious UV radiation. This naturally occurring photoprotective compound may have the potential to replace synthetic photoprotectants that can be used by the humans. https://sjst.psu.ac.th/journal/44-3/31.pdfscytonema sp. hkar-16maa palythinehplcesi-msraman spectra |
| spellingShingle | Sonal Mishra Niharika Sahu Neha Kumari Richa Rajeshwar P. Sinha Identification and characterization of mycosporine-like amino acid in the cyanobacterium Scytonema sp. HKAR-16 by HPLC, Raman spectroscopy, and ESI-MS Songklanakarin Journal of Science and Technology (SJST) scytonema sp. hkar-16 maa palythine hplc esi-ms raman spectra |
| title | Identification and characterization of mycosporine-like amino acid in the cyanobacterium Scytonema sp. HKAR-16 by HPLC, Raman spectroscopy, and ESI-MS |
| title_full | Identification and characterization of mycosporine-like amino acid in the cyanobacterium Scytonema sp. HKAR-16 by HPLC, Raman spectroscopy, and ESI-MS |
| title_fullStr | Identification and characterization of mycosporine-like amino acid in the cyanobacterium Scytonema sp. HKAR-16 by HPLC, Raman spectroscopy, and ESI-MS |
| title_full_unstemmed | Identification and characterization of mycosporine-like amino acid in the cyanobacterium Scytonema sp. HKAR-16 by HPLC, Raman spectroscopy, and ESI-MS |
| title_short | Identification and characterization of mycosporine-like amino acid in the cyanobacterium Scytonema sp. HKAR-16 by HPLC, Raman spectroscopy, and ESI-MS |
| title_sort | identification and characterization of mycosporine like amino acid in the cyanobacterium scytonema sp hkar 16 by hplc raman spectroscopy and esi ms |
| topic | scytonema sp. hkar-16 maa palythine hplc esi-ms raman spectra |
| url | https://sjst.psu.ac.th/journal/44-3/31.pdf |
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