Overexpression of Cassava <i>MeAnn2</i> Enhances the Salt and IAA Tolerance of Transgenic <i>Arabidopsis</i>
Annexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The <i>Arabidopsis thaliana AtAnn1</i> gene has been reported to play a significant role in various a...
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MDPI AG
2021-05-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/5/941 |
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| author | Xuejun Lin Ruimei Li Yangjiao Zhou Fenlian Tang Yajie Wang Xiaohua Lu Shijia Wang Yuan Yao Jiao Liu Xinwen Hu Jianchun Guo |
| author_facet | Xuejun Lin Ruimei Li Yangjiao Zhou Fenlian Tang Yajie Wang Xiaohua Lu Shijia Wang Yuan Yao Jiao Liu Xinwen Hu Jianchun Guo |
| author_sort | Xuejun Lin |
| collection | DOAJ |
| description | Annexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The <i>Arabidopsis thaliana AtAnn1</i> gene has been reported to play a significant role in various abiotic stress responses. In our study, the cDNA of an annexin gene highly similar to <i>AtAnn1</i> was isolated from the cassava genome and named <i>MeAnn2</i>. It contains domains specific to annexins, including four annexin repeat sequences (I–IV), a Ca<sup>2+</sup>-binding sequence, Ca<sup>2+</sup>-independent membrane-binding-related tryptophan residues, and a salt bridge-related domain. <i>MeAnn2</i> is localized in the cell membrane and cytoplasm, and it was found to be preferentially expressed in the storage roots of cassava. The overexpression of <i>MeAnn2</i> reduced the sensitivity of transgenic <i>Arabidopsis</i> to various Ca<sup>2+</sup>, NaCl, and indole-3-acetic acid (IAA) concentrations. The expression of the stress resistance-related gene <i>AtRD29B</i> and auxin signaling pathway-related genes <i>AtIAA4</i> and <i>AtLBD18</i> in transgenic <i>Arabidopsis</i> was significantly increased under salt stress, while the Malondialdehyde (MDA) content was significantly lower than that of the control. These results indicate that the <i>MeAnn2</i> gene may increase the salt tolerance of transgenic <i>Arabidopsis</i> via the IAA signaling pathway. |
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| language | English |
| last_indexed | 2024-03-10T11:36:32Z |
| publishDate | 2021-05-01 |
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| series | Plants |
| spelling | doaj.art-610e56f3a88f4474a192a1f7f23138ef2023-11-21T18:49:07ZengMDPI AGPlants2223-77472021-05-0110594110.3390/plants10050941Overexpression of Cassava <i>MeAnn2</i> Enhances the Salt and IAA Tolerance of Transgenic <i>Arabidopsis</i>Xuejun Lin0Ruimei Li1Yangjiao Zhou2Fenlian Tang3Yajie Wang4Xiaohua Lu5Shijia Wang6Yuan Yao7Jiao Liu8Xinwen Hu9Jianchun Guo10College of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaAnnexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The <i>Arabidopsis thaliana AtAnn1</i> gene has been reported to play a significant role in various abiotic stress responses. In our study, the cDNA of an annexin gene highly similar to <i>AtAnn1</i> was isolated from the cassava genome and named <i>MeAnn2</i>. It contains domains specific to annexins, including four annexin repeat sequences (I–IV), a Ca<sup>2+</sup>-binding sequence, Ca<sup>2+</sup>-independent membrane-binding-related tryptophan residues, and a salt bridge-related domain. <i>MeAnn2</i> is localized in the cell membrane and cytoplasm, and it was found to be preferentially expressed in the storage roots of cassava. The overexpression of <i>MeAnn2</i> reduced the sensitivity of transgenic <i>Arabidopsis</i> to various Ca<sup>2+</sup>, NaCl, and indole-3-acetic acid (IAA) concentrations. The expression of the stress resistance-related gene <i>AtRD29B</i> and auxin signaling pathway-related genes <i>AtIAA4</i> and <i>AtLBD18</i> in transgenic <i>Arabidopsis</i> was significantly increased under salt stress, while the Malondialdehyde (MDA) content was significantly lower than that of the control. These results indicate that the <i>MeAnn2</i> gene may increase the salt tolerance of transgenic <i>Arabidopsis</i> via the IAA signaling pathway.https://www.mdpi.com/2223-7747/10/5/941<i>Manihot esculenta</i>annexin<i>MeAnn2</i><i>Arabidopsis thaliana</i>genetic transformationstress analysis |
| spellingShingle | Xuejun Lin Ruimei Li Yangjiao Zhou Fenlian Tang Yajie Wang Xiaohua Lu Shijia Wang Yuan Yao Jiao Liu Xinwen Hu Jianchun Guo Overexpression of Cassava <i>MeAnn2</i> Enhances the Salt and IAA Tolerance of Transgenic <i>Arabidopsis</i> Plants <i>Manihot esculenta</i> annexin <i>MeAnn2</i> <i>Arabidopsis thaliana</i> genetic transformation stress analysis |
| title | Overexpression of Cassava <i>MeAnn2</i> Enhances the Salt and IAA Tolerance of Transgenic <i>Arabidopsis</i> |
| title_full | Overexpression of Cassava <i>MeAnn2</i> Enhances the Salt and IAA Tolerance of Transgenic <i>Arabidopsis</i> |
| title_fullStr | Overexpression of Cassava <i>MeAnn2</i> Enhances the Salt and IAA Tolerance of Transgenic <i>Arabidopsis</i> |
| title_full_unstemmed | Overexpression of Cassava <i>MeAnn2</i> Enhances the Salt and IAA Tolerance of Transgenic <i>Arabidopsis</i> |
| title_short | Overexpression of Cassava <i>MeAnn2</i> Enhances the Salt and IAA Tolerance of Transgenic <i>Arabidopsis</i> |
| title_sort | overexpression of cassava i meann2 i enhances the salt and iaa tolerance of transgenic i arabidopsis i |
| topic | <i>Manihot esculenta</i> annexin <i>MeAnn2</i> <i>Arabidopsis thaliana</i> genetic transformation stress analysis |
| url | https://www.mdpi.com/2223-7747/10/5/941 |
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