Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn
The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/17/12/14821 |
| _version_ | 1818495736428363776 |
|---|---|
| author | Daniele Del Rio Alan Crozier Renato Bruni Chiara Dall'Asta Gianni Galaverna Cristina García-Viguera Luca Calani Pedro Mena |
| author_facet | Daniele Del Rio Alan Crozier Renato Bruni Chiara Dall'Asta Gianni Galaverna Cristina García-Viguera Luca Calani Pedro Mena |
| author_sort | Daniele Del Rio |
| collection | DOAJ |
| description | The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds. |
| first_indexed | 2024-12-10T18:24:41Z |
| format | Article |
| id | doaj.art-610ff56c764c4e14b48fc4e38b0e4663 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T18:24:41Z |
| publishDate | 2012-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-610ff56c764c4e14b48fc4e38b0e46632022-12-22T01:38:06ZengMDPI AGMolecules1420-30492012-12-011712148211484010.3390/molecules171214821Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSnDaniele Del RioAlan CrozierRenato BruniChiara Dall'AstaGianni GalavernaCristina García-VigueraLuca CalaniPedro MenaThe comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.http://www.mdpi.com/1420-3049/17/12/14821Punica granatum L.source ionization conditionsellagitanninsC18 and PFP columnsphytochemicals identification |
| spellingShingle | Daniele Del Rio Alan Crozier Renato Bruni Chiara Dall'Asta Gianni Galaverna Cristina García-Viguera Luca Calani Pedro Mena Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn Molecules Punica granatum L. source ionization conditions ellagitannins C18 and PFP columns phytochemicals identification |
| title | Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn |
| title_full | Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn |
| title_fullStr | Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn |
| title_full_unstemmed | Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn |
| title_short | Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn |
| title_sort | rapid and comprehensive evaluation of poly phenolic compounds in pomegranate punica granatum l juice by uhplc msn |
| topic | Punica granatum L. source ionization conditions ellagitannins C18 and PFP columns phytochemicals identification |
| url | http://www.mdpi.com/1420-3049/17/12/14821 |
| work_keys_str_mv | AT danieledelrio rapidandcomprehensiveevaluationofpolyphenoliccompoundsinpomegranatepunicagranatumljuicebyuhplcmsn AT alancrozier rapidandcomprehensiveevaluationofpolyphenoliccompoundsinpomegranatepunicagranatumljuicebyuhplcmsn AT renatobruni rapidandcomprehensiveevaluationofpolyphenoliccompoundsinpomegranatepunicagranatumljuicebyuhplcmsn AT chiaradall039asta rapidandcomprehensiveevaluationofpolyphenoliccompoundsinpomegranatepunicagranatumljuicebyuhplcmsn AT giannigalaverna rapidandcomprehensiveevaluationofpolyphenoliccompoundsinpomegranatepunicagranatumljuicebyuhplcmsn AT cristinagarciaviguera rapidandcomprehensiveevaluationofpolyphenoliccompoundsinpomegranatepunicagranatumljuicebyuhplcmsn AT lucacalani rapidandcomprehensiveevaluationofpolyphenoliccompoundsinpomegranatepunicagranatumljuicebyuhplcmsn AT pedromena rapidandcomprehensiveevaluationofpolyphenoliccompoundsinpomegranatepunicagranatumljuicebyuhplcmsn |