Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis
Leptospirosis is an important environmental disease and a major threat to human health causing at least 1 million clinical infections annually. There has recently been a growing interest in understanding the environmental lifestyle of Leptospira. However, Leptospira isolation from complex environmen...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00816/full |
| _version_ | 1818130662415138816 |
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| author | Roman Thibeaux Dominique Girault Emilie Bierque Marie-Estelle Soupé-Gilbert Anna Rettinger Anthony Douyère Michael Meyer Gregorio Iraola Mathieu Picardeau Cyrille Goarant |
| author_facet | Roman Thibeaux Dominique Girault Emilie Bierque Marie-Estelle Soupé-Gilbert Anna Rettinger Anthony Douyère Michael Meyer Gregorio Iraola Mathieu Picardeau Cyrille Goarant |
| author_sort | Roman Thibeaux |
| collection | DOAJ |
| description | Leptospirosis is an important environmental disease and a major threat to human health causing at least 1 million clinical infections annually. There has recently been a growing interest in understanding the environmental lifestyle of Leptospira. However, Leptospira isolation from complex environmental samples is difficult and time-consuming and few tools are available to identify Leptospira isolates at the species level. Here, we propose a polyphasic isolation and identification scheme, which might prove useful to recover and identify environmental isolates and select those to be submitted to whole-genome sequencing. Using this approach, we recently described 12 novel Leptospira species for which we propose names. We also show that MALDI-ToF MS allows rapid and reliable identification and provide an extensive database of Leptospira MALDI-ToF mass spectra, which will be valuable to researchers in the leptospirosis community for species identification. Lastly, we also re-evaluate some of the current techniques for the molecular diagnosis of leptospirosis taking into account the extensive and recently revealed biodiversity of Leptospira in the environment. In conclusion, we describe our method for isolating Leptospira from the environment, confirm the usefulness of mass spectrometry for species identification and propose names for 12 novel species. This also offers the opportunity to refine current molecular diagnostic tools. |
| first_indexed | 2024-12-11T08:08:36Z |
| format | Article |
| id | doaj.art-61176491eccc46239afbffd389eb59af |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T08:08:36Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-61176491eccc46239afbffd389eb59af2022-12-22T01:14:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-05-01910.3389/fmicb.2018.00816360960Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the DiagnosisRoman Thibeaux0Dominique Girault1Emilie Bierque2Marie-Estelle Soupé-Gilbert3Anna Rettinger4Anthony Douyère5Michael Meyer6Gregorio Iraola7Mathieu Picardeau8Cyrille Goarant9Leptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New CaledoniaLeptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New CaledoniaLeptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New CaledoniaLeptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New CaledoniaBacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig Maximilian University of Munich, Munich, GermanyInstitut des Sciences Exactes et Appliquées, Plateau MET/MEB, Université de la Nouvelle-Calédonie, Noumea, New CaledoniaInstitut des Sciences Exactes et Appliquées, Plateau MET/MEB, Université de la Nouvelle-Calédonie, Noumea, New CaledoniaBioinformatics Unit, Institut Pasteur Montevideo, Montevideo, UruguayBiology of Spirochetes Unit, Institut Pasteur, National Reference Centre and WHO Collaborating Center for Leptospirosis, Paris, FranceLeptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New CaledoniaLeptospirosis is an important environmental disease and a major threat to human health causing at least 1 million clinical infections annually. There has recently been a growing interest in understanding the environmental lifestyle of Leptospira. However, Leptospira isolation from complex environmental samples is difficult and time-consuming and few tools are available to identify Leptospira isolates at the species level. Here, we propose a polyphasic isolation and identification scheme, which might prove useful to recover and identify environmental isolates and select those to be submitted to whole-genome sequencing. Using this approach, we recently described 12 novel Leptospira species for which we propose names. We also show that MALDI-ToF MS allows rapid and reliable identification and provide an extensive database of Leptospira MALDI-ToF mass spectra, which will be valuable to researchers in the leptospirosis community for species identification. Lastly, we also re-evaluate some of the current techniques for the molecular diagnosis of leptospirosis taking into account the extensive and recently revealed biodiversity of Leptospira in the environment. In conclusion, we describe our method for isolating Leptospira from the environment, confirm the usefulness of mass spectrometry for species identification and propose names for 12 novel species. This also offers the opportunity to refine current molecular diagnostic tools.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00816/fullsoil microbiologyMALDI-TOF MSWGS comparisonsnovel speciesisolation |
| spellingShingle | Roman Thibeaux Dominique Girault Emilie Bierque Marie-Estelle Soupé-Gilbert Anna Rettinger Anthony Douyère Michael Meyer Gregorio Iraola Mathieu Picardeau Cyrille Goarant Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis Frontiers in Microbiology soil microbiology MALDI-TOF MS WGS comparisons novel species isolation |
| title | Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis |
| title_full | Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis |
| title_fullStr | Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis |
| title_full_unstemmed | Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis |
| title_short | Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis |
| title_sort | biodiversity of environmental leptospira improving identification and revisiting the diagnosis |
| topic | soil microbiology MALDI-TOF MS WGS comparisons novel species isolation |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00816/full |
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