Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection
Objective: To establish an ion chromatography-integral pulsed amperometric detection for simultaneous determination of arabinose, galactose, mannose, glucose, ribose and lactose in Chinese yellow rice wine, and to discuss the contents of these sugars. Methods: The chromatographic separation was carr...
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The editorial department of Science and Technology of Food Industry
2022-02-01
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Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050209 |
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author | Nuo XU Zheyuan YAO Jinshui CHE Mingli YE Meilan CHEN |
author_facet | Nuo XU Zheyuan YAO Jinshui CHE Mingli YE Meilan CHEN |
author_sort | Nuo XU |
collection | DOAJ |
description | Objective: To establish an ion chromatography-integral pulsed amperometric detection for simultaneous determination of arabinose, galactose, mannose, glucose, ribose and lactose in Chinese yellow rice wine, and to discuss the contents of these sugars. Methods: The chromatographic separation was carried out on a CarbopActM10 (250 mm×4 mm) column with sodium hydroxide and anhydrate sodium acetate as eluents. The flow rate was 1.0 mL·min−1, and the column temperature was 30 ℃. The separation of six sugars was achieved within 20 min and the established method was used to determine the monosaccharide content in 26 yellow rice wine samples. Results: The reproducibility (RSD) of the method was less than 3.70%, the determination coefficient R2 was ≥0.9990, the recoveries were 91.6%~109.1%, and the lowest detection limit was 2.99×10−3~1.38×10−3 µg·mL−1. Conclusions: The main monosaccharide in yellow rice wine was glucose, and the contents of arabinose, galactose, mannose, ribose and lactose were low. The content of monosaccharide in semi-sweet yellow rice wine was higher than that in rice wine, and the difference was mainly related to brewing technology. |
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language | zho |
last_indexed | 2024-04-13T09:12:05Z |
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publisher | The editorial department of Science and Technology of Food Industry |
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series | Shipin gongye ke-ji |
spelling | doaj.art-6119060a52b44660aa84b3bf2357d0542022-12-22T02:52:51ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-02-0143325425910.13386/j.issn1002-0306.20210502092021050209-3Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric DetectionNuo XU0Zheyuan YAO1Jinshui CHE2Mingli YE3Meilan CHEN4College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaCollege of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaThemofisher Scientific College of Life Sciences, Shanghai 201203, ChinaCollege of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaCollege of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaObjective: To establish an ion chromatography-integral pulsed amperometric detection for simultaneous determination of arabinose, galactose, mannose, glucose, ribose and lactose in Chinese yellow rice wine, and to discuss the contents of these sugars. Methods: The chromatographic separation was carried out on a CarbopActM10 (250 mm×4 mm) column with sodium hydroxide and anhydrate sodium acetate as eluents. The flow rate was 1.0 mL·min−1, and the column temperature was 30 ℃. The separation of six sugars was achieved within 20 min and the established method was used to determine the monosaccharide content in 26 yellow rice wine samples. Results: The reproducibility (RSD) of the method was less than 3.70%, the determination coefficient R2 was ≥0.9990, the recoveries were 91.6%~109.1%, and the lowest detection limit was 2.99×10−3~1.38×10−3 µg·mL−1. Conclusions: The main monosaccharide in yellow rice wine was glucose, and the contents of arabinose, galactose, mannose, ribose and lactose were low. The content of monosaccharide in semi-sweet yellow rice wine was higher than that in rice wine, and the difference was mainly related to brewing technology.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050209ion chromatographyintegral pulsed amperometric detectionyellow rice winesimple sugars content |
spellingShingle | Nuo XU Zheyuan YAO Jinshui CHE Mingli YE Meilan CHEN Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection Shipin gongye ke-ji ion chromatography integral pulsed amperometric detection yellow rice wine simple sugars content |
title | Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection |
title_full | Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection |
title_fullStr | Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection |
title_full_unstemmed | Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection |
title_short | Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection |
title_sort | determination of arabinose galactose mannose glucose ribose and lactose in yellow rice wine by ion chromatography integral pulsed amperometric detection |
topic | ion chromatography integral pulsed amperometric detection yellow rice wine simple sugars content |
url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050209 |
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