Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection

Objective: To establish an ion chromatography-integral pulsed amperometric detection for simultaneous determination of arabinose, galactose, mannose, glucose, ribose and lactose in Chinese yellow rice wine, and to discuss the contents of these sugars. Methods: The chromatographic separation was carr...

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Main Authors: Nuo XU, Zheyuan YAO, Jinshui CHE, Mingli YE, Meilan CHEN
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2022-02-01
Series:Shipin gongye ke-ji
Subjects:
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050209
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author Nuo XU
Zheyuan YAO
Jinshui CHE
Mingli YE
Meilan CHEN
author_facet Nuo XU
Zheyuan YAO
Jinshui CHE
Mingli YE
Meilan CHEN
author_sort Nuo XU
collection DOAJ
description Objective: To establish an ion chromatography-integral pulsed amperometric detection for simultaneous determination of arabinose, galactose, mannose, glucose, ribose and lactose in Chinese yellow rice wine, and to discuss the contents of these sugars. Methods: The chromatographic separation was carried out on a CarbopActM10 (250 mm×4 mm) column with sodium hydroxide and anhydrate sodium acetate as eluents. The flow rate was 1.0 mL·min−1, and the column temperature was 30 ℃. The separation of six sugars was achieved within 20 min and the established method was used to determine the monosaccharide content in 26 yellow rice wine samples. Results: The reproducibility (RSD) of the method was less than 3.70%, the determination coefficient R2 was ≥0.9990, the recoveries were 91.6%~109.1%, and the lowest detection limit was 2.99×10−3~1.38×10−3 µg·mL−1. Conclusions: The main monosaccharide in yellow rice wine was glucose, and the contents of arabinose, galactose, mannose, ribose and lactose were low. The content of monosaccharide in semi-sweet yellow rice wine was higher than that in rice wine, and the difference was mainly related to brewing technology.
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spelling doaj.art-6119060a52b44660aa84b3bf2357d0542022-12-22T02:52:51ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-02-0143325425910.13386/j.issn1002-0306.20210502092021050209-3Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric DetectionNuo XU0Zheyuan YAO1Jinshui CHE2Mingli YE3Meilan CHEN4College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaCollege of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaThemofisher Scientific College of Life Sciences, Shanghai 201203, ChinaCollege of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaCollege of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaObjective: To establish an ion chromatography-integral pulsed amperometric detection for simultaneous determination of arabinose, galactose, mannose, glucose, ribose and lactose in Chinese yellow rice wine, and to discuss the contents of these sugars. Methods: The chromatographic separation was carried out on a CarbopActM10 (250 mm×4 mm) column with sodium hydroxide and anhydrate sodium acetate as eluents. The flow rate was 1.0 mL·min−1, and the column temperature was 30 ℃. The separation of six sugars was achieved within 20 min and the established method was used to determine the monosaccharide content in 26 yellow rice wine samples. Results: The reproducibility (RSD) of the method was less than 3.70%, the determination coefficient R2 was ≥0.9990, the recoveries were 91.6%~109.1%, and the lowest detection limit was 2.99×10−3~1.38×10−3 µg·mL−1. Conclusions: The main monosaccharide in yellow rice wine was glucose, and the contents of arabinose, galactose, mannose, ribose and lactose were low. The content of monosaccharide in semi-sweet yellow rice wine was higher than that in rice wine, and the difference was mainly related to brewing technology.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050209ion chromatographyintegral pulsed amperometric detectionyellow rice winesimple sugars content
spellingShingle Nuo XU
Zheyuan YAO
Jinshui CHE
Mingli YE
Meilan CHEN
Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection
Shipin gongye ke-ji
ion chromatography
integral pulsed amperometric detection
yellow rice wine
simple sugars content
title Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection
title_full Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection
title_fullStr Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection
title_full_unstemmed Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection
title_short Determination of Arabinose, Galactose, Mannose, Glucose, Ribose and Lactose in Yellow Rice Wine by Ion Chromatography-Integral Pulsed Amperometric Detection
title_sort determination of arabinose galactose mannose glucose ribose and lactose in yellow rice wine by ion chromatography integral pulsed amperometric detection
topic ion chromatography
integral pulsed amperometric detection
yellow rice wine
simple sugars content
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050209
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