Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro study
Introduction: Root treatment will not be successful, without a proper root canal irrigation with less disadvantages and antibacterial effect. The aim of this study was to compare antimicrobial effect of cedar extract and 2.5%NaOCl on E. faecalis. Materials & Methods: In disk diffusion test, a st...
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| Format: | Article |
| Language: | English |
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Babol University of Medical Sciences
2018-03-01
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| Series: | Caspian journal of dental research |
| Subjects: | |
| Online Access: | http://cjdr.ir/browse.php?a_code=A-10-299-1&slc_lang=en&sid=1 |
| _version_ | 1828833379439607808 |
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| author | Akam Saeidi Mahmoud Reza Hamidi Abolfazl Davoodabadi Elham Mahmoudi Soraya Khafri Zahra Nikouee Rad |
| author_facet | Akam Saeidi Mahmoud Reza Hamidi Abolfazl Davoodabadi Elham Mahmoudi Soraya Khafri Zahra Nikouee Rad |
| author_sort | Akam Saeidi |
| collection | DOAJ |
| description | Introduction: Root treatment will not be successful, without a proper root canal irrigation with less disadvantages and antibacterial effect. The aim of this study was to compare antimicrobial effect of cedar extract and 2.5%NaOCl on E. faecalis.
Materials & Methods: In disk diffusion test, a standard suspension of E. faecalis (ATCC 29212) was cultured on plate and different concentrations (0.05, 0.15, 0.25, 0.35, 0.45 g/ml) of methanolic or hydro-alcoholic extracts, 2.5% NaOCl and physiologic serum (as negative control) were infused on paper disks. The inhibition zone measured after 48 h. In microdilution test, serial dilution of methanolic and hydro-alcoholic extracts, 2.5% NaOCl and physiologic serum in 1:2 proportion was performed in Brain Heart Infusion (BHI) culture medium. Then, standard suspension of E. faecalis was added to each well of micro plate. Data were analyzed using ANOVA.
Results: Hydro-alcoholic and methanolic extracts had antibacterial effect on E. faecalis. Inhibition zone of 2.5% NaOCl was significantly higher than that of other extracts (p<0.001). In microdilution test, E. faecalis bacterium was sensitive to both hydro-alcoholic and methanolic extracts but it was more sensitive to 2.5% NaOCl.
Conclusion: Totally, 2.5% NaOCl had the highest antibacterial effect on E.faecalis followed by hydro-alcoholic and methanolic extracts. NaOCl is an effective irrigant in root treatment until the studies like this can find a good alternative for it. |
| first_indexed | 2024-12-12T17:18:31Z |
| format | Article |
| id | doaj.art-611ab0b157894d84aaebc7fab44fdbc7 |
| institution | Directory Open Access Journal |
| issn | 2251-9890 2322-2395 |
| language | English |
| last_indexed | 2024-12-12T17:18:31Z |
| publishDate | 2018-03-01 |
| publisher | Babol University of Medical Sciences |
| record_format | Article |
| series | Caspian journal of dental research |
| spelling | doaj.art-611ab0b157894d84aaebc7fab44fdbc72022-12-22T00:17:44ZengBabol University of Medical SciencesCaspian journal of dental research2251-98902322-23952018-03-01713742Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro studyAkam Saeidi0Mahmoud Reza Hamidi1Abolfazl Davoodabadi2Elham Mahmoudi3Soraya Khafri4Zahra Nikouee Rad5 ,Dental Materials Research Center, Health Research Institute, Department of Endodontics, Babol University of Medical Sciences , Department of Endodontics, Faculty of Dentistry, Babol University of Medical Sciences ,Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences ,Department of Endodontics, Faculty of Dentistry, Babol University of Medical Sciences ,Department of Biostatics and Epidemiology, Faculty of Medicine, Babol University of Medical Sciences ,Student Research Committee, Babol University of Medical Sciences Introduction: Root treatment will not be successful, without a proper root canal irrigation with less disadvantages and antibacterial effect. The aim of this study was to compare antimicrobial effect of cedar extract and 2.5%NaOCl on E. faecalis. Materials & Methods: In disk diffusion test, a standard suspension of E. faecalis (ATCC 29212) was cultured on plate and different concentrations (0.05, 0.15, 0.25, 0.35, 0.45 g/ml) of methanolic or hydro-alcoholic extracts, 2.5% NaOCl and physiologic serum (as negative control) were infused on paper disks. The inhibition zone measured after 48 h. In microdilution test, serial dilution of methanolic and hydro-alcoholic extracts, 2.5% NaOCl and physiologic serum in 1:2 proportion was performed in Brain Heart Infusion (BHI) culture medium. Then, standard suspension of E. faecalis was added to each well of micro plate. Data were analyzed using ANOVA. Results: Hydro-alcoholic and methanolic extracts had antibacterial effect on E. faecalis. Inhibition zone of 2.5% NaOCl was significantly higher than that of other extracts (p<0.001). In microdilution test, E. faecalis bacterium was sensitive to both hydro-alcoholic and methanolic extracts but it was more sensitive to 2.5% NaOCl. Conclusion: Totally, 2.5% NaOCl had the highest antibacterial effect on E.faecalis followed by hydro-alcoholic and methanolic extracts. NaOCl is an effective irrigant in root treatment until the studies like this can find a good alternative for it.http://cjdr.ir/browse.php?a_code=A-10-299-1&slc_lang=en&sid=1Enterococcus faecalis Methanol Sodium hypochlorite Ziziphus |
| spellingShingle | Akam Saeidi Mahmoud Reza Hamidi Abolfazl Davoodabadi Elham Mahmoudi Soraya Khafri Zahra Nikouee Rad Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro study Caspian journal of dental research Enterococcus faecalis Methanol Sodium hypochlorite Ziziphus |
| title | Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro study |
| title_full | Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro study |
| title_fullStr | Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro study |
| title_full_unstemmed | Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro study |
| title_short | Comparison of antibacterial effect of methanolic and hydro-alcoholic ziziphus spina-christi extract with 2.5% sodium hypochlorite on enterococcus faecalis: an in vitro study |
| title_sort | comparison of antibacterial effect of methanolic and hydro alcoholic ziziphus spina christi extract with 2 5 sodium hypochlorite on enterococcus faecalis an in vitro study |
| topic | Enterococcus faecalis Methanol Sodium hypochlorite Ziziphus |
| url | http://cjdr.ir/browse.php?a_code=A-10-299-1&slc_lang=en&sid=1 |
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