Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro Study
Tifoid fever caused by Salmonella typhi (S. typhi) is endemic disease in Indonesia. S. typhi begin to resist to chloramphenicol, so it is necessary to study about natural material as traditional medicine such as tempuyung. The aim of this research was to study antibacterial activity and Minimum Inh...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Jember, Faculty of Medicine
2016-06-01
|
| Series: | Journal of Agromedicine and Medical Sciences (AMS) |
| Online Access: | https://jurnal.unej.ac.id/index.php/JAMS/article/view/2777 |
| _version_ | 1811173092715659264 |
|---|---|
| author | Rinda Yanuarisa Dini Agustina Ali Santosa |
| author_facet | Rinda Yanuarisa Dini Agustina Ali Santosa |
| author_sort | Rinda Yanuarisa |
| collection | DOAJ |
| description | Tifoid fever caused by Salmonella typhi (S. typhi) is endemic disease in Indonesia. S. typhi begin to resist to chloramphenicol, so it is necessary to study about natural material as traditional medicine such as tempuyung. The aim of this research was to study antibacterial activity and Minimum Inhibitory Concentration (MIC) of ethanolic extract from tempuyung leaf (Sonchus arvensis L.) againts growth of S. typhi. This research used quasy experimental methods with post test only control group design. Treatment consisted of 8 concentrations with 5 replications was 2,5 µg/disc, 5 µg/disc, 10 µg/disc, 20 µg/disc, 30 µg/disc, 40 µg/disc, 60 µg/disc, and 80 µg/disc. As much as 10 µl from each concentration was dropped into disc and placed in Mueller Hinton Agar. Then incubated for 18 hours to 37oC. Observation of bacterial inhibition zone by using a caliper. Result of this research that the leaf extract at a concentration 10 µg/disc, 20 µg/disc, 30 µg/disc, 40 µg/disc, 60 µg/disc, and 80 µg/disc can inhibit the growth of Salmonella typhi. Minimum Inhibitory Concentration of ethanolic extract from tempuyung leaf was 10 µg/disc qualitatively and 4,43 µg/disc quantitatively.
Key words: Sonchus arvensis L., Salmonella typhi, antibacterial |
| first_indexed | 2024-04-10T17:42:08Z |
| format | Article |
| id | doaj.art-611ad714af4c4775a031a6c0cd2ddb94 |
| institution | Directory Open Access Journal |
| issn | 2460-9048 2714-5654 |
| language | English |
| last_indexed | 2024-04-10T17:42:08Z |
| publishDate | 2016-06-01 |
| publisher | University of Jember, Faculty of Medicine |
| record_format | Article |
| series | Journal of Agromedicine and Medical Sciences (AMS) |
| spelling | doaj.art-611ad714af4c4775a031a6c0cd2ddb942023-02-03T12:17:32ZengUniversity of Jember, Faculty of MedicineJournal of Agromedicine and Medical Sciences (AMS)2460-90482714-56542016-06-0122162777Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro StudyRinda Yanuarisa0Dini Agustina1Ali SantosaProgram Studi Pendidikan Dokter Fakultas Kedokteran Univeritas JemberLaboratorium Mikrobiologi Fakultas Kedokteran, Universitas JemberTifoid fever caused by Salmonella typhi (S. typhi) is endemic disease in Indonesia. S. typhi begin to resist to chloramphenicol, so it is necessary to study about natural material as traditional medicine such as tempuyung. The aim of this research was to study antibacterial activity and Minimum Inhibitory Concentration (MIC) of ethanolic extract from tempuyung leaf (Sonchus arvensis L.) againts growth of S. typhi. This research used quasy experimental methods with post test only control group design. Treatment consisted of 8 concentrations with 5 replications was 2,5 µg/disc, 5 µg/disc, 10 µg/disc, 20 µg/disc, 30 µg/disc, 40 µg/disc, 60 µg/disc, and 80 µg/disc. As much as 10 µl from each concentration was dropped into disc and placed in Mueller Hinton Agar. Then incubated for 18 hours to 37oC. Observation of bacterial inhibition zone by using a caliper. Result of this research that the leaf extract at a concentration 10 µg/disc, 20 µg/disc, 30 µg/disc, 40 µg/disc, 60 µg/disc, and 80 µg/disc can inhibit the growth of Salmonella typhi. Minimum Inhibitory Concentration of ethanolic extract from tempuyung leaf was 10 µg/disc qualitatively and 4,43 µg/disc quantitatively. Key words: Sonchus arvensis L., Salmonella typhi, antibacterialhttps://jurnal.unej.ac.id/index.php/JAMS/article/view/2777 |
| spellingShingle | Rinda Yanuarisa Dini Agustina Ali Santosa Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro Study Journal of Agromedicine and Medical Sciences (AMS) |
| title | Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro Study |
| title_full | Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro Study |
| title_fullStr | Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro Study |
| title_full_unstemmed | Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro Study |
| title_short | Antibacterial Activity of Ethanolic Extract from Tempuyung Leaf (Sonchus arvensis L.) againts Salmonella typhi by In Vitro Study |
| title_sort | antibacterial activity of ethanolic extract from tempuyung leaf sonchus arvensis l againts salmonella typhi by in vitro study |
| url | https://jurnal.unej.ac.id/index.php/JAMS/article/view/2777 |
| work_keys_str_mv | AT rindayanuarisa antibacterialactivityofethanolicextractfromtempuyungleafsonchusarvensislagaintssalmonellatyphibyinvitrostudy AT diniagustina antibacterialactivityofethanolicextractfromtempuyungleafsonchusarvensislagaintssalmonellatyphibyinvitrostudy AT alisantosa antibacterialactivityofethanolicextractfromtempuyungleafsonchusarvensislagaintssalmonellatyphibyinvitrostudy |