The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass
Enzyme-assisted valorization of lichenan represents a green and sustainable alternative to the conventional chemical industry. The recently discovered lytic polysaccharide monooxygenases (LPMOs) are essential components of state-of-the-art enzyme cocktails for lichenin bioconversion. The LPMOs named...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.970540/full |
| _version_ | 1811341369862520832 |
|---|---|
| author | Lixi Cai Lixi Cai Lixi Cai Ying Zheng Yunmeng Chu Yuanqing Lin Lixing Liu Lixing Liu Guangya Zhang |
| author_facet | Lixi Cai Lixi Cai Lixi Cai Ying Zheng Yunmeng Chu Yuanqing Lin Lixing Liu Lixing Liu Guangya Zhang |
| author_sort | Lixi Cai |
| collection | DOAJ |
| description | Enzyme-assisted valorization of lichenan represents a green and sustainable alternative to the conventional chemical industry. The recently discovered lytic polysaccharide monooxygenases (LPMOs) are essential components of state-of-the-art enzyme cocktails for lichenin bioconversion. The LPMOs named SpyTag fused LPMOs (AST) from Chaetomium globosum was functionally expressed in E. coli and exhibited 1.25-fold synergism with lichenase, whereas AST alone produced no detectable reducing sugars. HPLC results further confirm that AST does not alter the endogenous hydrolysis mode of lichenase but rather enhances its hydrolysis efficiency by disrupting the long chain of lichenan and releasing more reducing ends. To the best of our knowledge, this was the first report on the synergistic effect of LPMOs and lichenase, which may have great synergistic potential in the conversion of lichen biomass. Furthermore, a novel strategy for the covalently immobilizing AST and lichenase on silica nanoparticles (SNPs) from the cell lysate in a single step was proposed, which exhibited high activity recovery (82.9%) and high immobilization yield (94.8%). After 12 independent runs, about 67.4 % of the initial activity of the immobilized enzymes was retained. The resulted biocatalyst systems exhibited the green and sustainable strategy in the bioconversion of lichen biomass as well as other diverse polysaccharides. |
| first_indexed | 2024-04-13T18:54:32Z |
| format | Article |
| id | doaj.art-611e7dbf8b9a4b9eb5cbf5fe907a3490 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T18:54:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-611e7dbf8b9a4b9eb5cbf5fe907a34902022-12-22T02:34:18ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-10-01910.3389/fnut.2022.970540970540The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomassLixi Cai0Lixi Cai1Lixi Cai2Ying Zheng3Yunmeng Chu4Yuanqing Lin5Lixing Liu6Lixing Liu7Guangya Zhang8College of Basic Medicine, Putian University, Putian, ChinaDepartment of Bioengineering and Biotechnology, Huaqiao University, Xiamen, ChinaKey Laboratory of Translational Tumor Medicine in Fujian Province, Putian University, Putian, ChinaCollege of Pharmaceutical and Medical Technology, Putian University, Putian, ChinaDepartment of Bioengineering and Biotechnology, Huaqiao University, Xiamen, ChinaDepartment of Bioengineering and Biotechnology, Huaqiao University, Xiamen, ChinaCollege of Basic Medicine, Putian University, Putian, ChinaKey Laboratory of Translational Tumor Medicine in Fujian Province, Putian University, Putian, ChinaDepartment of Bioengineering and Biotechnology, Huaqiao University, Xiamen, ChinaEnzyme-assisted valorization of lichenan represents a green and sustainable alternative to the conventional chemical industry. The recently discovered lytic polysaccharide monooxygenases (LPMOs) are essential components of state-of-the-art enzyme cocktails for lichenin bioconversion. The LPMOs named SpyTag fused LPMOs (AST) from Chaetomium globosum was functionally expressed in E. coli and exhibited 1.25-fold synergism with lichenase, whereas AST alone produced no detectable reducing sugars. HPLC results further confirm that AST does not alter the endogenous hydrolysis mode of lichenase but rather enhances its hydrolysis efficiency by disrupting the long chain of lichenan and releasing more reducing ends. To the best of our knowledge, this was the first report on the synergistic effect of LPMOs and lichenase, which may have great synergistic potential in the conversion of lichen biomass. Furthermore, a novel strategy for the covalently immobilizing AST and lichenase on silica nanoparticles (SNPs) from the cell lysate in a single step was proposed, which exhibited high activity recovery (82.9%) and high immobilization yield (94.8%). After 12 independent runs, about 67.4 % of the initial activity of the immobilized enzymes was retained. The resulted biocatalyst systems exhibited the green and sustainable strategy in the bioconversion of lichen biomass as well as other diverse polysaccharides.https://www.frontiersin.org/articles/10.3389/fnut.2022.970540/fulllytic polysaccharide monooxygenasesynergistic effectlichenasesilica nanoparticlesmulti-enzyme immobilization |
| spellingShingle | Lixi Cai Lixi Cai Lixi Cai Ying Zheng Yunmeng Chu Yuanqing Lin Lixing Liu Lixing Liu Guangya Zhang The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass Frontiers in Nutrition lytic polysaccharide monooxygenase synergistic effect lichenase silica nanoparticles multi-enzyme immobilization |
| title | The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass |
| title_full | The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass |
| title_fullStr | The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass |
| title_full_unstemmed | The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass |
| title_short | The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass |
| title_sort | synergism of lytic polysaccharide monooxygenases with lichenase and their co immobilization on silica nanospheres for green conversion of lichen biomass |
| topic | lytic polysaccharide monooxygenase synergistic effect lichenase silica nanoparticles multi-enzyme immobilization |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.970540/full |
| work_keys_str_mv | AT lixicai thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixicai thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixicai thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT yingzheng thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT yunmengchu thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT yuanqinglin thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixingliu thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixingliu thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT guangyazhang thesynergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixicai synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixicai synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixicai synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT yingzheng synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT yunmengchu synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT yuanqinglin synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixingliu synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT lixingliu synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass AT guangyazhang synergismoflyticpolysaccharidemonooxygenaseswithlichenaseandtheircoimmobilizationonsilicananospheresforgreenconversionoflichenbiomass |