Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam.
Two distinct Phytophthora taxa were found to be associated with brown rot of pomelo (Citrus grandis), a new disease of this ancestral Citrus species, in the Vinh Long province, Mekong River Delta area, southern Vietnam. On the basis of morphological characters and using the ITS1-5.8S-ITS2 region of...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5313238?pdf=render |
| _version_ | 1798043199858540544 |
|---|---|
| author | Ivana Puglisi Alessandro De Patrizio Leonardo Schena Thomas Jung Maria Evoli Antonella Pane Nguyen Van Hoa Mai Van Tri Sandra Wright Mauritz Ramstedt Christer Olsson Roberto Faedda Gaetano Magnano di San Lio Santa Olga Cacciola |
| author_facet | Ivana Puglisi Alessandro De Patrizio Leonardo Schena Thomas Jung Maria Evoli Antonella Pane Nguyen Van Hoa Mai Van Tri Sandra Wright Mauritz Ramstedt Christer Olsson Roberto Faedda Gaetano Magnano di San Lio Santa Olga Cacciola |
| author_sort | Ivana Puglisi |
| collection | DOAJ |
| description | Two distinct Phytophthora taxa were found to be associated with brown rot of pomelo (Citrus grandis), a new disease of this ancestral Citrus species, in the Vinh Long province, Mekong River Delta area, southern Vietnam. On the basis of morphological characters and using the ITS1-5.8S-ITS2 region of the rDNA and the cytochrome oxidase subunit 1 (COI) as barcode genes, one of the two taxa was provisionally named as Phytophthora sp. prodigiosa, being closely related to but distinct from P. insolita, a species in Phytophthora Clade 9, while the other one, was closely related to but distinct from the Clade 2 species P. meadii and was informally designated as Phytophthora sp. mekongensis. Isolates of P. sp. prodigiosa and P. sp. mekongensis were also obtained from necrotic fibrous roots of Volkamer lemon (C. volkameriana) rootstocks grafted with 'King' mandarin (Citrus nobilis) and from trees of pomelo, respectively, in other provinces of the Mekong River Delta, indicating a widespread occurrence of both Phytophthora species in this citrus-growing area. Koch's postulates were fulfilled via pathogenicity tests on fruits of various Citrus species, including pomelo, grapefruit (Citrus x paradisi), sweet orange (Citrus x sinensis) and bergamot (Citrus x bergamia) as well as on the rootstock of 2-year-old trees of pomelo and sweet orange on 'Carrizo' citrange (C. sinensis 'Washington Navel' x Poncirus trifoliata). This is the first report of a Phytophthora species from Clade 2 other than P. citricola and P. citrophthora as causal agent of fruit brown rot of Citrus worldwide and the first report of P. insolita complex in Vietnam. Results indicate that likely Vietnam is still an unexplored reservoir of Phytophthora diversity. |
| first_indexed | 2024-04-11T22:46:09Z |
| format | Article |
| id | doaj.art-611ecc81bc194eaf98ac615840b36a85 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T22:46:09Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-611ecc81bc194eaf98ac615840b36a852022-12-22T03:58:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017208510.1371/journal.pone.0172085Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam.Ivana PuglisiAlessandro De PatrizioLeonardo SchenaThomas JungMaria EvoliAntonella PaneNguyen Van HoaMai Van TriSandra WrightMauritz RamstedtChrister OlssonRoberto FaeddaGaetano Magnano di San LioSanta Olga CacciolaTwo distinct Phytophthora taxa were found to be associated with brown rot of pomelo (Citrus grandis), a new disease of this ancestral Citrus species, in the Vinh Long province, Mekong River Delta area, southern Vietnam. On the basis of morphological characters and using the ITS1-5.8S-ITS2 region of the rDNA and the cytochrome oxidase subunit 1 (COI) as barcode genes, one of the two taxa was provisionally named as Phytophthora sp. prodigiosa, being closely related to but distinct from P. insolita, a species in Phytophthora Clade 9, while the other one, was closely related to but distinct from the Clade 2 species P. meadii and was informally designated as Phytophthora sp. mekongensis. Isolates of P. sp. prodigiosa and P. sp. mekongensis were also obtained from necrotic fibrous roots of Volkamer lemon (C. volkameriana) rootstocks grafted with 'King' mandarin (Citrus nobilis) and from trees of pomelo, respectively, in other provinces of the Mekong River Delta, indicating a widespread occurrence of both Phytophthora species in this citrus-growing area. Koch's postulates were fulfilled via pathogenicity tests on fruits of various Citrus species, including pomelo, grapefruit (Citrus x paradisi), sweet orange (Citrus x sinensis) and bergamot (Citrus x bergamia) as well as on the rootstock of 2-year-old trees of pomelo and sweet orange on 'Carrizo' citrange (C. sinensis 'Washington Navel' x Poncirus trifoliata). This is the first report of a Phytophthora species from Clade 2 other than P. citricola and P. citrophthora as causal agent of fruit brown rot of Citrus worldwide and the first report of P. insolita complex in Vietnam. Results indicate that likely Vietnam is still an unexplored reservoir of Phytophthora diversity.http://europepmc.org/articles/PMC5313238?pdf=render |
| spellingShingle | Ivana Puglisi Alessandro De Patrizio Leonardo Schena Thomas Jung Maria Evoli Antonella Pane Nguyen Van Hoa Mai Van Tri Sandra Wright Mauritz Ramstedt Christer Olsson Roberto Faedda Gaetano Magnano di San Lio Santa Olga Cacciola Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam. PLoS ONE |
| title | Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam. |
| title_full | Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam. |
| title_fullStr | Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam. |
| title_full_unstemmed | Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam. |
| title_short | Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam. |
| title_sort | two previously unknown phytophthora species associated with brown rot of pomelo citrus grandis fruits in vietnam |
| url | http://europepmc.org/articles/PMC5313238?pdf=render |
| work_keys_str_mv | AT ivanapuglisi twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT alessandrodepatrizio twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT leonardoschena twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT thomasjung twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT mariaevoli twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT antonellapane twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT nguyenvanhoa twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT maivantri twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT sandrawright twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT mauritzramstedt twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT christerolsson twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT robertofaedda twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT gaetanomagnanodisanlio twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam AT santaolgacacciola twopreviouslyunknownphytophthoraspeciesassociatedwithbrownrotofpomelocitrusgrandisfruitsinvietnam |