Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles Outbreak
The rapid transmission of measles poses a great challenge for measles elimination. Thus, rapid testing is required to screen the health status in the population during measles outbreaks. A pseudotype-based virus neutralisation assay was used to measure neutralising antibody titres in serum samples c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1716 |
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| author | Siyuan Hu Nicola Logan Sarah Coleman Cariad Evans Brian J. Willett Margaret J. Hosie |
| author_facet | Siyuan Hu Nicola Logan Sarah Coleman Cariad Evans Brian J. Willett Margaret J. Hosie |
| author_sort | Siyuan Hu |
| collection | DOAJ |
| description | The rapid transmission of measles poses a great challenge for measles elimination. Thus, rapid testing is required to screen the health status in the population during measles outbreaks. A pseudotype-based virus neutralisation assay was used to measure neutralising antibody titres in serum samples collected from healthcare workers in Sheffield during the measles outbreak in 2016. Vesicular stomatitis virus (VSV) pseudotypes bearing the haemagglutinin and fusion glycoproteins of measles virus (MeV) and carrying a luciferase marker gene were prepared; the neutralising antibody titre was defined as the dilution resulting in 90% reduction in luciferase activity. Spearman’s correlation coefficients between IgG titres and neutralising antibody levels ranged from 0.40 to 0.55 (<i>p</i> < 0.05) or from 0.71 to 0.79 (<i>p</i> < 0.0001) when the IgG titres were obtained using different testing kits. In addition, the currently used vaccine was observed to cross-neutralise most circulating MeV genotypes. However, the percentage of individuals being “well-protected” was lower than 95%, the target rate of vaccination coverage to eliminate measles. These results demonstrate that the level of clinical protection against measles in individuals could be inferred by IgG titre, as long as a precise correlation has been established between IgG testing and neutralisation assay; moreover, maintaining a high vaccination coverage rate is still necessary for measles elimination. |
| first_indexed | 2024-03-09T12:22:45Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:22:45Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-611ed35e95114fb597717b46f808ea9d2023-11-30T22:39:06ZengMDPI AGViruses1999-49152022-08-01148171610.3390/v14081716Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles OutbreakSiyuan Hu0Nicola Logan1Sarah Coleman2Cariad Evans3Brian J. Willett4Margaret J. Hosie5MRC-University of Glasgow Centre for Virus Research, Bearsden Road, Glasgow G61 1QH, UKMRC-University of Glasgow Centre for Virus Research, Bearsden Road, Glasgow G61 1QH, UKVirology Department, Sheffield Teaching Hospitals NHS Foundation Trust, Northern General Hospital, Herries Road, Sheffield S5 7AU, UKVirology Department, Sheffield Teaching Hospitals NHS Foundation Trust, Northern General Hospital, Herries Road, Sheffield S5 7AU, UKMRC-University of Glasgow Centre for Virus Research, Bearsden Road, Glasgow G61 1QH, UKMRC-University of Glasgow Centre for Virus Research, Bearsden Road, Glasgow G61 1QH, UKThe rapid transmission of measles poses a great challenge for measles elimination. Thus, rapid testing is required to screen the health status in the population during measles outbreaks. A pseudotype-based virus neutralisation assay was used to measure neutralising antibody titres in serum samples collected from healthcare workers in Sheffield during the measles outbreak in 2016. Vesicular stomatitis virus (VSV) pseudotypes bearing the haemagglutinin and fusion glycoproteins of measles virus (MeV) and carrying a luciferase marker gene were prepared; the neutralising antibody titre was defined as the dilution resulting in 90% reduction in luciferase activity. Spearman’s correlation coefficients between IgG titres and neutralising antibody levels ranged from 0.40 to 0.55 (<i>p</i> < 0.05) or from 0.71 to 0.79 (<i>p</i> < 0.0001) when the IgG titres were obtained using different testing kits. In addition, the currently used vaccine was observed to cross-neutralise most circulating MeV genotypes. However, the percentage of individuals being “well-protected” was lower than 95%, the target rate of vaccination coverage to eliminate measles. These results demonstrate that the level of clinical protection against measles in individuals could be inferred by IgG titre, as long as a precise correlation has been established between IgG testing and neutralisation assay; moreover, maintaining a high vaccination coverage rate is still necessary for measles elimination.https://www.mdpi.com/1999-4915/14/8/1716measles outbreakhealthcare workersneutralising antibodyclinical protectionIgG testing |
| spellingShingle | Siyuan Hu Nicola Logan Sarah Coleman Cariad Evans Brian J. Willett Margaret J. Hosie Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles Outbreak Viruses measles outbreak healthcare workers neutralising antibody clinical protection IgG testing |
| title | Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles Outbreak |
| title_full | Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles Outbreak |
| title_fullStr | Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles Outbreak |
| title_full_unstemmed | Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles Outbreak |
| title_short | Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles Outbreak |
| title_sort | correlating igg levels with neutralising antibody levels to indicate clinical protection in healthcare workers at risk during a measles outbreak |
| topic | measles outbreak healthcare workers neutralising antibody clinical protection IgG testing |
| url | https://www.mdpi.com/1999-4915/14/8/1716 |
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