Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization
When the body is under pathological stress (injury or disease), the status of associated acupoints changes, including decreased pain threshold. Such changes in acupoint from a “silent” to an “active” state are considered “acupoint sensitization,” which has become an important indicator of acupoint s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2022.974007/full |
| _version_ | 1818083665690755072 |
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| author | Wanrong Li Wanrong Li Wanrong Li Wanrong Li Jia Liu Aiwen Chen Aiwen Chen Aiwen Chen Aiwen Chen Danqing Dai Danqing Dai Danqing Dai Danqing Dai Tiantian Zhao Tiantian Zhao Tiantian Zhao Tiantian Zhao Qiong Liu Qiong Liu Qiong Liu Qiong Liu Jianren Song Lize Xiong Lize Xiong Lize Xiong Lize Xiong Xiao-Fei Gao Xiao-Fei Gao Xiao-Fei Gao Xiao-Fei Gao |
| author_facet | Wanrong Li Wanrong Li Wanrong Li Wanrong Li Jia Liu Aiwen Chen Aiwen Chen Aiwen Chen Aiwen Chen Danqing Dai Danqing Dai Danqing Dai Danqing Dai Tiantian Zhao Tiantian Zhao Tiantian Zhao Tiantian Zhao Qiong Liu Qiong Liu Qiong Liu Qiong Liu Jianren Song Lize Xiong Lize Xiong Lize Xiong Lize Xiong Xiao-Fei Gao Xiao-Fei Gao Xiao-Fei Gao Xiao-Fei Gao |
| author_sort | Wanrong Li |
| collection | DOAJ |
| description | When the body is under pathological stress (injury or disease), the status of associated acupoints changes, including decreased pain threshold. Such changes in acupoint from a “silent” to an “active” state are considered “acupoint sensitization,” which has become an important indicator of acupoint selection. However, the mechanism of acupoint sensitization remains unclear. In this study, by retrograde tracing, morphological, chemogenetic, and behavioral methods, we found there are some dorsal root ganglion (DRG) neurons innervating the ST36 acupoint and ipsilateral hind paw (IHP) plantar simultaneously. Inhibition of these shared neurons induced analgesia in the complete Freund’s adjuvant (CFA) pain model and obstruction of nociceptive sensation in normal mice, and elevated the mechanical pain threshold (MPT) of ST36 acupoint in the CFA model. Excitation of shared neurons induced pain and declined the MPT of ST36 acupoint. Furthermore, most of the shared DRG neurons express TRPV1, a marker of nociceptive neurons. These results indicate that the shared nociceptive DRG neurons participate in ST36 acupoint sensitization in CFA-induced chronic pain. This raised a neural mechanism of acupoint sensitization at the level of primary sensory transmission. |
| first_indexed | 2024-12-10T19:41:37Z |
| format | Article |
| id | doaj.art-61218481efd4458d8f5c7e45df2f48ad |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-10T19:41:37Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-61218481efd4458d8f5c7e45df2f48ad2022-12-22T01:35:59ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992022-08-011510.3389/fnmol.2022.974007974007Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitizationWanrong Li0Wanrong Li1Wanrong Li2Wanrong Li3Jia Liu4Aiwen Chen5Aiwen Chen6Aiwen Chen7Aiwen Chen8Danqing Dai9Danqing Dai10Danqing Dai11Danqing Dai12Tiantian Zhao13Tiantian Zhao14Tiantian Zhao15Tiantian Zhao16Qiong Liu17Qiong Liu18Qiong Liu19Qiong Liu20Jianren Song21Lize Xiong22Lize Xiong23Lize Xiong24Lize Xiong25Xiao-Fei Gao26Xiao-Fei Gao27Xiao-Fei Gao28Xiao-Fei Gao29Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaClinical Research Center for Anesthesiology and Perioperative Medicine, Tongji University, Shanghai, ChinaDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaShanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai, ChinaFaculty of Anesthesiology, Changhai Hospital, Naval Medical University, Shanghai, ChinaTranslational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaClinical Research Center for Anesthesiology and Perioperative Medicine, Tongji University, Shanghai, ChinaDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaShanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai, ChinaTranslational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaClinical Research Center for Anesthesiology and Perioperative Medicine, Tongji University, Shanghai, ChinaDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaShanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai, ChinaTranslational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaClinical Research Center for Anesthesiology and Perioperative Medicine, Tongji University, Shanghai, ChinaDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaShanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai, ChinaTranslational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaClinical Research Center for Anesthesiology and Perioperative Medicine, Tongji University, Shanghai, ChinaDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaShanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai, ChinaTranslational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaTranslational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaClinical Research Center for Anesthesiology and Perioperative Medicine, Tongji University, Shanghai, ChinaDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaShanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai, ChinaTranslational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaClinical Research Center for Anesthesiology and Perioperative Medicine, Tongji University, Shanghai, ChinaDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, ChinaShanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai, ChinaWhen the body is under pathological stress (injury or disease), the status of associated acupoints changes, including decreased pain threshold. Such changes in acupoint from a “silent” to an “active” state are considered “acupoint sensitization,” which has become an important indicator of acupoint selection. However, the mechanism of acupoint sensitization remains unclear. In this study, by retrograde tracing, morphological, chemogenetic, and behavioral methods, we found there are some dorsal root ganglion (DRG) neurons innervating the ST36 acupoint and ipsilateral hind paw (IHP) plantar simultaneously. Inhibition of these shared neurons induced analgesia in the complete Freund’s adjuvant (CFA) pain model and obstruction of nociceptive sensation in normal mice, and elevated the mechanical pain threshold (MPT) of ST36 acupoint in the CFA model. Excitation of shared neurons induced pain and declined the MPT of ST36 acupoint. Furthermore, most of the shared DRG neurons express TRPV1, a marker of nociceptive neurons. These results indicate that the shared nociceptive DRG neurons participate in ST36 acupoint sensitization in CFA-induced chronic pain. This raised a neural mechanism of acupoint sensitization at the level of primary sensory transmission.https://www.frontiersin.org/articles/10.3389/fnmol.2022.974007/fullpaindorsal root ganglionacupoint sensitizationST36 acupointnociceptive neuronacupuncture |
| spellingShingle | Wanrong Li Wanrong Li Wanrong Li Wanrong Li Jia Liu Aiwen Chen Aiwen Chen Aiwen Chen Aiwen Chen Danqing Dai Danqing Dai Danqing Dai Danqing Dai Tiantian Zhao Tiantian Zhao Tiantian Zhao Tiantian Zhao Qiong Liu Qiong Liu Qiong Liu Qiong Liu Jianren Song Lize Xiong Lize Xiong Lize Xiong Lize Xiong Xiao-Fei Gao Xiao-Fei Gao Xiao-Fei Gao Xiao-Fei Gao Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization Frontiers in Molecular Neuroscience pain dorsal root ganglion acupoint sensitization ST36 acupoint nociceptive neuron acupuncture |
| title | Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization |
| title_full | Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization |
| title_fullStr | Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization |
| title_full_unstemmed | Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization |
| title_short | Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization |
| title_sort | shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization |
| topic | pain dorsal root ganglion acupoint sensitization ST36 acupoint nociceptive neuron acupuncture |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2022.974007/full |
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