Metabolites of Tryptophane and Phenylalanine as Markers of Small Bowel Ischemia-Reperfusion Injury
Ischemic-reperfusion injury of the small intestine is an acute clinical condition with high mortality rate. This study describes the changes in levels of phenylalanine and tryptophan metabolites in intestinal homogenates and urine samples of Wistar male rats after 60 minutes of mesenteric ischemia a...
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| Format: | Article |
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De Gruyter
2018-08-01
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| Series: | Open Chemistry |
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| Online Access: | http://www.degruyter.com/view/j/chem.2018.16.issue-1/chem-2018-0076/chem-2018-0076.xml?format=INT |
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| author | Mašlanková Jana Tóth Štefan Tomečková Vladimíra Tóth Tímea Katz Matan Veselá Jarmila Hertelyová Zdenka Schnitzer Marek Živčák Jozef Mareková Mária |
| author_facet | Mašlanková Jana Tóth Štefan Tomečková Vladimíra Tóth Tímea Katz Matan Veselá Jarmila Hertelyová Zdenka Schnitzer Marek Živčák Jozef Mareková Mária |
| author_sort | Mašlanková Jana |
| collection | DOAJ |
| description | Ischemic-reperfusion injury of the small intestine is an acute clinical condition with high mortality rate. This study describes the changes in levels of phenylalanine and tryptophan metabolites in intestinal homogenates and urine samples of Wistar male rats after 60 minutes of mesenteric ischemia and different reperfusion periods (1, 24, 30 hours) in comparison with a control group without the ischemia. The ischemic-reperfusion injury was quantified by the histopathological injury index. The levels of serotonin, epinephrine, and norepinephrine were determined in the intestinal homogenate and epinephrine, vanillylmandelic acid, and the 5-hydroxyindoleacetic acid was analyzed in urine using the HPLC method. The statistically significant increased level of serotonin, epinephrine and norepinephrine were detected in the intestinal samples after 24 hours of reperfusion (p<0.01); even the most elevated serotonin level was observed after one hour of reperfusion (p<0.001). A statistically significant decreased level of vanillylmandelic acid was observed after one hour of reperfusion, but it significantly increased after 24 hours (p<0.05) in urine. The elevated level of the 5-hydroxyindoleacetic acid after one hour and 24 hours after reperfusion (p<0.05) were determined in the urine. The most significant elevated epinephrine level was observed after 24 hours of reperfusion (p<0.001) in urine. Results showed a potential role of serotonin as an early biomarker (after one hour of reperfusion) of small intestinal damaged homogenate, while the best predictor of intestinal injury in urine was epinephrine, which was elevated after 24 hours. |
| first_indexed | 2024-12-12T02:08:21Z |
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| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-12-12T02:08:21Z |
| publishDate | 2018-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-6126340e68ef4dc1b01d711996b017bd2022-12-22T00:41:59ZengDe GruyterOpen Chemistry2391-54202018-08-0116170971510.1515/chem-2018-0076chem-2018-0076Metabolites of Tryptophane and Phenylalanine as Markers of Small Bowel Ischemia-Reperfusion InjuryMašlanková Jana0Tóth Štefan1Tomečková Vladimíra2Tóth Tímea3Katz Matan4Veselá Jarmila5Hertelyová Zdenka6Schnitzer Marek7Živčák Jozef8Mareková Mária9Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, 04011Košice, Slovakia1st Department of Internal Medicine, Pavol Jozef Safarik University and Louis Pasteur University Hospital, Trieda SNP 1, 041 90Košice, SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, 041 90Košice, Slovakia1st Department of Internal Medicine, Pavol Jozef Safarik University and Louis Pasteur University Hospital, Trieda SNP 1, 041 90Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, 04011Košice, SlovakiaDepartment of Histology and Embryology, Faculty of Medicine, Pavol Jozef Šafárik University, Šrobárová 2, 04180Košice, Slovakia1st Department of Experimental Medicine, Faculty of Medicine, Pavol Jozef Safarik University, Trieda SNP 1, 041 90Košice, SlovakiaDepartment of biomedical engineering and measurements, Faculty of mechanical engineering, Technical university of Kosice, Letna 9, Kosice04200, SlovakiaDepartment of biomedical engineering and measurements, Faculty of mechanical engineering, Technical university of Kosice, Letna 9, Kosice04200, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, 04011Košice, SlovakiaIschemic-reperfusion injury of the small intestine is an acute clinical condition with high mortality rate. This study describes the changes in levels of phenylalanine and tryptophan metabolites in intestinal homogenates and urine samples of Wistar male rats after 60 minutes of mesenteric ischemia and different reperfusion periods (1, 24, 30 hours) in comparison with a control group without the ischemia. The ischemic-reperfusion injury was quantified by the histopathological injury index. The levels of serotonin, epinephrine, and norepinephrine were determined in the intestinal homogenate and epinephrine, vanillylmandelic acid, and the 5-hydroxyindoleacetic acid was analyzed in urine using the HPLC method. The statistically significant increased level of serotonin, epinephrine and norepinephrine were detected in the intestinal samples after 24 hours of reperfusion (p<0.01); even the most elevated serotonin level was observed after one hour of reperfusion (p<0.001). A statistically significant decreased level of vanillylmandelic acid was observed after one hour of reperfusion, but it significantly increased after 24 hours (p<0.05) in urine. The elevated level of the 5-hydroxyindoleacetic acid after one hour and 24 hours after reperfusion (p<0.05) were determined in the urine. The most significant elevated epinephrine level was observed after 24 hours of reperfusion (p<0.001) in urine. Results showed a potential role of serotonin as an early biomarker (after one hour of reperfusion) of small intestinal damaged homogenate, while the best predictor of intestinal injury in urine was epinephrine, which was elevated after 24 hours.http://www.degruyter.com/view/j/chem.2018.16.issue-1/chem-2018-0076/chem-2018-0076.xml?format=INTischemic-reperfusion injury of small intestineserotonincatecholamines |
| spellingShingle | Mašlanková Jana Tóth Štefan Tomečková Vladimíra Tóth Tímea Katz Matan Veselá Jarmila Hertelyová Zdenka Schnitzer Marek Živčák Jozef Mareková Mária Metabolites of Tryptophane and Phenylalanine as Markers of Small Bowel Ischemia-Reperfusion Injury Open Chemistry ischemic-reperfusion injury of small intestine serotonin catecholamines |
| title | Metabolites of Tryptophane and Phenylalanine as Markers of Small Bowel Ischemia-Reperfusion Injury |
| title_full | Metabolites of Tryptophane and Phenylalanine as Markers of Small Bowel Ischemia-Reperfusion Injury |
| title_fullStr | Metabolites of Tryptophane and Phenylalanine as Markers of Small Bowel Ischemia-Reperfusion Injury |
| title_full_unstemmed | Metabolites of Tryptophane and Phenylalanine as Markers of Small Bowel Ischemia-Reperfusion Injury |
| title_short | Metabolites of Tryptophane and Phenylalanine as Markers of Small Bowel Ischemia-Reperfusion Injury |
| title_sort | metabolites of tryptophane and phenylalanine as markers of small bowel ischemia reperfusion injury |
| topic | ischemic-reperfusion injury of small intestine serotonin catecholamines |
| url | http://www.degruyter.com/view/j/chem.2018.16.issue-1/chem-2018-0076/chem-2018-0076.xml?format=INT |
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