Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings
The present research work is focused on the design and investigation of electrospun composite membranes based on citric acid-functionalized chitosan (CsA) containing reduced graphene oxide-tetraethylene pentamine (CsA/rGO-TEPA) as materials with opportune bio-properties for applications in wound dre...
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MDPI AG
2022-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/2/294 |
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| author | Elena Cojocaru Jana Ghitman Gratiela Gradisteanu Pircalabioru Cristina Stavarache Andrada Serafim Eugeniu Vasile Horia Iovu |
| author_facet | Elena Cojocaru Jana Ghitman Gratiela Gradisteanu Pircalabioru Cristina Stavarache Andrada Serafim Eugeniu Vasile Horia Iovu |
| author_sort | Elena Cojocaru |
| collection | DOAJ |
| description | The present research work is focused on the design and investigation of electrospun composite membranes based on citric acid-functionalized chitosan (CsA) containing reduced graphene oxide-tetraethylene pentamine (CsA/rGO-TEPA) as materials with opportune bio-properties for applications in wound dressings. The covalent functionalization of chitosan (CS) with citric acid (CA) was achieved through the EDC/NHS coupling system and was checked by <sup>1</sup>H-NMR spectroscopy and FTIR spectrometry. The mixtures to be electrospun were formulated by adding three concentrations of rGO-TEPA into the 1/1 (<i>w</i>/<i>w</i>) CsA/poly (ethylene oxide) (PEO) solution. The effect of rGO-TEPA concentration on the morphology, wettability, thermal stability, cytocompatibility, cytotoxicity, and anti-biofilm activity of the nanofibrous membranes was extensively investigated. FTIR and Raman results confirmed the covalent and non-covalent interactions that appeared between the system’s compounds, and the exfoliation of rGO-TEPA sheets within the CsA in the presence of PEO (CsA/P) polymer matrix, respectively. SEM analysis emphasized the nanofibrous architecture of membranes and the presence of rGO-TEPA sheets entrapped into the CsA nanofiber structure. The MTT cellular viability assay showed a good cytocompatibility with the highest level of cell development and proliferation registered for the CsA/P composite nanofibrous membrane with 0.250 wt.% rGO-TEPA. The designed nanofibrous membranes could have potential applications in wound dressings, given that they showed a good anti-biofilm activity against Gram-negative <i>Pseudomonas aeruginosa</i> and Gram-positive <i>Staphylococcus aureus</i> bacterial strains. |
| first_indexed | 2024-03-10T00:40:17Z |
| format | Article |
| id | doaj.art-612c183ca16d4c40ad72bb687c307e91 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T00:40:17Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-612c183ca16d4c40ad72bb687c307e912023-11-23T15:09:13ZengMDPI AGPolymers2073-43602022-01-0114229410.3390/polym14020294Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound DressingsElena Cojocaru0Jana Ghitman1Gratiela Gradisteanu Pircalabioru2Cristina Stavarache3Andrada Serafim4Eugeniu Vasile5Horia Iovu6Advanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaAdvanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaResearch Institute of the University of Bucharest (ICUB), University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaAdvanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaAdvanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaDepartment of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaAdvanced Polymer Materials Group, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaThe present research work is focused on the design and investigation of electrospun composite membranes based on citric acid-functionalized chitosan (CsA) containing reduced graphene oxide-tetraethylene pentamine (CsA/rGO-TEPA) as materials with opportune bio-properties for applications in wound dressings. The covalent functionalization of chitosan (CS) with citric acid (CA) was achieved through the EDC/NHS coupling system and was checked by <sup>1</sup>H-NMR spectroscopy and FTIR spectrometry. The mixtures to be electrospun were formulated by adding three concentrations of rGO-TEPA into the 1/1 (<i>w</i>/<i>w</i>) CsA/poly (ethylene oxide) (PEO) solution. The effect of rGO-TEPA concentration on the morphology, wettability, thermal stability, cytocompatibility, cytotoxicity, and anti-biofilm activity of the nanofibrous membranes was extensively investigated. FTIR and Raman results confirmed the covalent and non-covalent interactions that appeared between the system’s compounds, and the exfoliation of rGO-TEPA sheets within the CsA in the presence of PEO (CsA/P) polymer matrix, respectively. SEM analysis emphasized the nanofibrous architecture of membranes and the presence of rGO-TEPA sheets entrapped into the CsA nanofiber structure. The MTT cellular viability assay showed a good cytocompatibility with the highest level of cell development and proliferation registered for the CsA/P composite nanofibrous membrane with 0.250 wt.% rGO-TEPA. The designed nanofibrous membranes could have potential applications in wound dressings, given that they showed a good anti-biofilm activity against Gram-negative <i>Pseudomonas aeruginosa</i> and Gram-positive <i>Staphylococcus aureus</i> bacterial strains.https://www.mdpi.com/2073-4360/14/2/294citric acid-functionalized chitosanrGO-TEPAnanofibrous architecturein vitro cytocompatibilityanti-biofilm activity |
| spellingShingle | Elena Cojocaru Jana Ghitman Gratiela Gradisteanu Pircalabioru Cristina Stavarache Andrada Serafim Eugeniu Vasile Horia Iovu Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings Polymers citric acid-functionalized chitosan rGO-TEPA nanofibrous architecture in vitro cytocompatibility anti-biofilm activity |
| title | Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings |
| title_full | Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings |
| title_fullStr | Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings |
| title_full_unstemmed | Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings |
| title_short | Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings |
| title_sort | electrospun nanofibrous membranes based on citric acid functionalized chitosan containing rgo tepa with potential application in wound dressings |
| topic | citric acid-functionalized chitosan rGO-TEPA nanofibrous architecture in vitro cytocompatibility anti-biofilm activity |
| url | https://www.mdpi.com/2073-4360/14/2/294 |
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