Characterization of collagen response to bone fracture healing using polarization-SHG
Abstract In this study, we extend on the three parameter analysis approach of utilizing a noninvasive dual-liquid–crystal-based polarization-resolved second harmonic generation (SHG) microscopy to facilitate the quantitative characterization of collagen types I and II in fracture healing tissues. Th...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21876-z |
| _version_ | 1811328715042324480 |
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| author | Anupama Nair Shu-Chun Chuang Yi-Shan Lin Chung-Hwan Chen Ting-Chen Fang Hsiao-Chi Chiu Chi-Hsiang Lien Shean-Jen Chen |
| author_facet | Anupama Nair Shu-Chun Chuang Yi-Shan Lin Chung-Hwan Chen Ting-Chen Fang Hsiao-Chi Chiu Chi-Hsiang Lien Shean-Jen Chen |
| author_sort | Anupama Nair |
| collection | DOAJ |
| description | Abstract In this study, we extend on the three parameter analysis approach of utilizing a noninvasive dual-liquid–crystal-based polarization-resolved second harmonic generation (SHG) microscopy to facilitate the quantitative characterization of collagen types I and II in fracture healing tissues. The SHG images under various linear and circular polarization states are analyzed and quantified in terms of the peptide pitch angle (PA), SHG-circular dichroism (CD), and anisotropy parameter (AP). The results show that the collagen PA has a value of 49.26° after 2 weeks of fracture healing (collagen type II domination) and 49.05° after 4 weeks (collagen type I domination). Moreover, the SHG-CD and AP values of the different collagen types differ by 0.05. The change tendencies of the extracted PA, SHG-CD, and AP parameters over the healing time are consistent with the collagen properties of healthy nonfractured bone. Thus, the feasibility of the proposed dual-liquid–crystal-based polarization-SHG method for differentiating between collagen types I and II in bone fracture healing tissue is confirmed. |
| first_indexed | 2024-04-13T15:29:59Z |
| format | Article |
| id | doaj.art-612da861af6344b881e7f2c9e117cfd6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T15:29:59Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-612da861af6344b881e7f2c9e117cfd62022-12-22T02:41:24ZengNature PortfolioScientific Reports2045-23222022-11-0112111210.1038/s41598-022-21876-zCharacterization of collagen response to bone fracture healing using polarization-SHGAnupama Nair0Shu-Chun Chuang1Yi-Shan Lin2Chung-Hwan Chen3Ting-Chen Fang4Hsiao-Chi Chiu5Chi-Hsiang Lien6Shean-Jen Chen7College of Photonics, National Yang Ming Chiao Tung UniversityOrthopaedic Research Center, Kaohsiung Medical UniversityOrthopaedic Research Center, Kaohsiung Medical UniversityOrthopaedic Research Center, Kaohsiung Medical UniversitySchool of Medicine, Kaohsiung Medical UniversityOrthopaedic Research Center, Kaohsiung Medical UniversityDepartment of Mechanical Engineering, National United UniversityCollege of Photonics, National Yang Ming Chiao Tung UniversityAbstract In this study, we extend on the three parameter analysis approach of utilizing a noninvasive dual-liquid–crystal-based polarization-resolved second harmonic generation (SHG) microscopy to facilitate the quantitative characterization of collagen types I and II in fracture healing tissues. The SHG images under various linear and circular polarization states are analyzed and quantified in terms of the peptide pitch angle (PA), SHG-circular dichroism (CD), and anisotropy parameter (AP). The results show that the collagen PA has a value of 49.26° after 2 weeks of fracture healing (collagen type II domination) and 49.05° after 4 weeks (collagen type I domination). Moreover, the SHG-CD and AP values of the different collagen types differ by 0.05. The change tendencies of the extracted PA, SHG-CD, and AP parameters over the healing time are consistent with the collagen properties of healthy nonfractured bone. Thus, the feasibility of the proposed dual-liquid–crystal-based polarization-SHG method for differentiating between collagen types I and II in bone fracture healing tissue is confirmed.https://doi.org/10.1038/s41598-022-21876-z |
| spellingShingle | Anupama Nair Shu-Chun Chuang Yi-Shan Lin Chung-Hwan Chen Ting-Chen Fang Hsiao-Chi Chiu Chi-Hsiang Lien Shean-Jen Chen Characterization of collagen response to bone fracture healing using polarization-SHG Scientific Reports |
| title | Characterization of collagen response to bone fracture healing using polarization-SHG |
| title_full | Characterization of collagen response to bone fracture healing using polarization-SHG |
| title_fullStr | Characterization of collagen response to bone fracture healing using polarization-SHG |
| title_full_unstemmed | Characterization of collagen response to bone fracture healing using polarization-SHG |
| title_short | Characterization of collagen response to bone fracture healing using polarization-SHG |
| title_sort | characterization of collagen response to bone fracture healing using polarization shg |
| url | https://doi.org/10.1038/s41598-022-21876-z |
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