Micropropagation of Trichopilia suavis Lindl. & Paxton
Trichopilia suavis is one of the important representatives of the genus Trichopilia, which can be used as a potted plant for the interior design. also, its beautiful inflorescences are added unusual element to bouquet compositions and actively used in the production of perfumes. This study was carr...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Floricultura e Plantas Ornamentais
2023-09-01
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| Series: | Ornamental Horticulture |
| Subjects: | |
| Online Access: | https://rbho.emnuvens.com.br/rbho/article/view/2653 |
| _version_ | 1797689563924135936 |
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| author | Muthab Hussien Olga Molkanova Irina Vjacheslavovna Mitrofanova |
| author_facet | Muthab Hussien Olga Molkanova Irina Vjacheslavovna Mitrofanova |
| author_sort | Muthab Hussien |
| collection | DOAJ |
| description |
Trichopilia suavis is one of the important representatives of the genus Trichopilia, which can be used as a potted plant for the interior design. also, its beautiful inflorescences are added unusual element to bouquet compositions and actively used in the production of perfumes. This study was carried out in order to develop and improve micropropagation method for mass clonal production of T. suavis through protocorm culture. Half-strength Murashige and Skoog culture medium (½) has been modified by adding different organic components and growth regulators. The protocorms obtained from seeds germinated by asymbiotic method were used as explants. The results showed that the optimal treatment for formation of new protocorms (7.50 ± 0.7 units per protocorm) was obtained by culture on ½ MS medium with 0.5 mg L-1 of 6-Benzylaminopurine (6-BAP) and 100 mL L-1 of coconut water. At the stage of rooting, the highest number and length of roots (7.0 ± 0.45 units per plantlet, 2.64 ± 0.18 cm) were obtained through subsequent cultivation of plantlets on ½ MS medium with 0.5 mg L-1 indolyl-3-butyric acid (IBA), 1.0 g L-1 charcoal with the addition of 50 g L-1 of banana puree. The obtained seedlings were successfully adapted in a substrate consisting of bark, perlite and peat in a ratio of 1:1:1.
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| first_indexed | 2024-03-12T01:47:23Z |
| format | Article |
| id | doaj.art-61307b81ad8c44f8bde73f3896b17f02 |
| institution | Directory Open Access Journal |
| issn | 2447-536X |
| language | English |
| last_indexed | 2024-03-12T01:47:23Z |
| publishDate | 2023-09-01 |
| publisher | Sociedade Brasileira de Floricultura e Plantas Ornamentais |
| record_format | Article |
| series | Ornamental Horticulture |
| spelling | doaj.art-61307b81ad8c44f8bde73f3896b17f022023-09-08T21:01:18ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2023-09-0129310.1590/2447-536X.v29i3.2653Micropropagation of Trichopilia suavis Lindl. & PaxtonMuthab Hussien0Olga Molkanova1Irina Vjacheslavovna Mitrofanova2Moscow Timiryazev Agricultural Academy, Russian State Agrarian UniversityTsitsin Main Botanical Garden of The Russian Academy of SciencesTsitsin Main Botanical Garden of The Russian Academy of Sciences Trichopilia suavis is one of the important representatives of the genus Trichopilia, which can be used as a potted plant for the interior design. also, its beautiful inflorescences are added unusual element to bouquet compositions and actively used in the production of perfumes. This study was carried out in order to develop and improve micropropagation method for mass clonal production of T. suavis through protocorm culture. Half-strength Murashige and Skoog culture medium (½) has been modified by adding different organic components and growth regulators. The protocorms obtained from seeds germinated by asymbiotic method were used as explants. The results showed that the optimal treatment for formation of new protocorms (7.50 ± 0.7 units per protocorm) was obtained by culture on ½ MS medium with 0.5 mg L-1 of 6-Benzylaminopurine (6-BAP) and 100 mL L-1 of coconut water. At the stage of rooting, the highest number and length of roots (7.0 ± 0.45 units per plantlet, 2.64 ± 0.18 cm) were obtained through subsequent cultivation of plantlets on ½ MS medium with 0.5 mg L-1 indolyl-3-butyric acid (IBA), 1.0 g L-1 charcoal with the addition of 50 g L-1 of banana puree. The obtained seedlings were successfully adapted in a substrate consisting of bark, perlite and peat in a ratio of 1:1:1. https://rbho.emnuvens.com.br/rbho/article/view/2653Orchidprotocormgrowth regulatororganic additivein vitro |
| spellingShingle | Muthab Hussien Olga Molkanova Irina Vjacheslavovna Mitrofanova Micropropagation of Trichopilia suavis Lindl. & Paxton Ornamental Horticulture Orchid protocorm growth regulator organic additive in vitro |
| title | Micropropagation of Trichopilia suavis Lindl. & Paxton |
| title_full | Micropropagation of Trichopilia suavis Lindl. & Paxton |
| title_fullStr | Micropropagation of Trichopilia suavis Lindl. & Paxton |
| title_full_unstemmed | Micropropagation of Trichopilia suavis Lindl. & Paxton |
| title_short | Micropropagation of Trichopilia suavis Lindl. & Paxton |
| title_sort | micropropagation of trichopilia suavis lindl paxton |
| topic | Orchid protocorm growth regulator organic additive in vitro |
| url | https://rbho.emnuvens.com.br/rbho/article/view/2653 |
| work_keys_str_mv | AT muthabhussien micropropagationoftrichopiliasuavislindlpaxton AT olgamolkanova micropropagationoftrichopiliasuavislindlpaxton AT irinavjacheslavovnamitrofanova micropropagationoftrichopiliasuavislindlpaxton |