Estimating Real-Time qPCR Amplification Efficiency from Single-Reaction Data
Methods for estimating the qPCR amplification efficiency <i>E</i> from data for single reactions are tested on six multireplicate datasets, with emphasis on their performance as a function of the range of cycles <i>n</i><sub>1</sub>–<i>n</i><sub>...
Main Author: | Joel Tellinghuisen |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-07-01
|
Series: | Life |
Subjects: | |
Online Access: | https://www.mdpi.com/2075-1729/11/7/693 |
Similar Items
-
Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!
by: Joel Tellinghuisen
Published: (2020-07-01) -
Laboratory Exercise to Measure Plasmid Copy Number by qPCR
by: Benjamin David, et al.
Published: (2021-09-01) -
qPCR multiplex detection of microRNA and messenger RNA in a single reaction
by: Samantha Khoury, et al.
Published: (2020-06-01) -
qPCR and loop mediated isothermal amplification for rapid detection of Ustilago tritici
by: Hanwen Yan, et al.
Published: (2019-09-01) -
rdml: A Mathematica package for parsing and importing Real-Time qPCR data
by: Ramiro Magno, et al.
Published: (2017-06-01)