Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker rat
IntroductionThe gastrointestinal tract plays a major role in regulating glucose homeostasis and gut endocrine function. The current study examines the effects of Roux-en-Y gastric bypass (RYGB) on intestinal GLP-1, glucose transporter expression and function in the obese Zucker rat (ZR).MethodsTwo g...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.901984/full |
| _version_ | 1811322160555229184 |
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| author | Qinghe Meng Derek M. Culnan Tamer Ahmed Mingjie Sun Robert N. Cooney |
| author_facet | Qinghe Meng Derek M. Culnan Tamer Ahmed Mingjie Sun Robert N. Cooney |
| author_sort | Qinghe Meng |
| collection | DOAJ |
| description | IntroductionThe gastrointestinal tract plays a major role in regulating glucose homeostasis and gut endocrine function. The current study examines the effects of Roux-en-Y gastric bypass (RYGB) on intestinal GLP-1, glucose transporter expression and function in the obese Zucker rat (ZR).MethodsTwo groups of ZRs were studied: RYGB and sham surgery pair-fed (PF) fed rats. Body weight and food intake were measured daily. On post-operative day (POD) 21, an oral glucose test (OGT) was performed, basal and 30-minute plasma, portal venous glucose and glucagon-like peptide-1 (GLP-1) levels were measured. In separate ZRs, the biliopancreatic, Roux limb (Roux) and common channel (CC) intestinal segments were harvested on POD 21.ResultsBody weight was decreased in the RYGB group. Basal and 30-minute OGT plasma and portal glucose levels were decreased after RYGB. Basal plasma GLP-1 levels were similar, while a 4.5-fold increase in GLP-1 level was observed in 30-minute after RYGB (vs. PF). The increase in basal and 30-minute portal venous GLP-1 levels after RYGB were accompanied by increased mRNA expressions of proglucagon and PC 1/3, GPR119 protein in the Roux and CC segments. mRNA and protein levels of FFAR2/3 were increased in Roux segment. RYGB decreased brush border glucose transport, transporter proteins (SGLT1 and GLUT2) and mRNA levels of Tas1R1/Tas1R3 and α-gustducin in the Roux and CC segments.ConclusionsReductions in intestinal glucose transport and enhanced post-prandial GLP-1 release were associated with increases in GRP119 and FFAR2/3 after RYGB in the ZR model. Post-RYGB reductions in the regulation of intestinal glucose transport and L cell receptors regulating GLP-1 secretion represent potential mechanisms for improved glycemic control. |
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| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-13T13:30:24Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-6133ba176a1842879aad745eb9f9f0e72022-12-22T02:45:00ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-08-011310.3389/fendo.2022.901984901984Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker ratQinghe Meng0Derek M. Culnan1Tamer Ahmed2Mingjie Sun3Robert N. Cooney4Department of Surgery, State University of New York (SUNY), Upstate Medical University, Syracuse, NY, United StatesBurn and Reconstructive Centers of America, Jackson, MS, United StatesDepartment of Surgery, State University of New York (SUNY), Upstate Medical University, Syracuse, NY, United StatesDepartment of Surgery, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Surgery, State University of New York (SUNY), Upstate Medical University, Syracuse, NY, United StatesIntroductionThe gastrointestinal tract plays a major role in regulating glucose homeostasis and gut endocrine function. The current study examines the effects of Roux-en-Y gastric bypass (RYGB) on intestinal GLP-1, glucose transporter expression and function in the obese Zucker rat (ZR).MethodsTwo groups of ZRs were studied: RYGB and sham surgery pair-fed (PF) fed rats. Body weight and food intake were measured daily. On post-operative day (POD) 21, an oral glucose test (OGT) was performed, basal and 30-minute plasma, portal venous glucose and glucagon-like peptide-1 (GLP-1) levels were measured. In separate ZRs, the biliopancreatic, Roux limb (Roux) and common channel (CC) intestinal segments were harvested on POD 21.ResultsBody weight was decreased in the RYGB group. Basal and 30-minute OGT plasma and portal glucose levels were decreased after RYGB. Basal plasma GLP-1 levels were similar, while a 4.5-fold increase in GLP-1 level was observed in 30-minute after RYGB (vs. PF). The increase in basal and 30-minute portal venous GLP-1 levels after RYGB were accompanied by increased mRNA expressions of proglucagon and PC 1/3, GPR119 protein in the Roux and CC segments. mRNA and protein levels of FFAR2/3 were increased in Roux segment. RYGB decreased brush border glucose transport, transporter proteins (SGLT1 and GLUT2) and mRNA levels of Tas1R1/Tas1R3 and α-gustducin in the Roux and CC segments.ConclusionsReductions in intestinal glucose transport and enhanced post-prandial GLP-1 release were associated with increases in GRP119 and FFAR2/3 after RYGB in the ZR model. Post-RYGB reductions in the regulation of intestinal glucose transport and L cell receptors regulating GLP-1 secretion represent potential mechanisms for improved glycemic control.https://www.frontiersin.org/articles/10.3389/fendo.2022.901984/fullRYGBGLP-1SGLT1GLUT2TAS1R1TAS1R3 |
| spellingShingle | Qinghe Meng Derek M. Culnan Tamer Ahmed Mingjie Sun Robert N. Cooney Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker rat Frontiers in Endocrinology RYGB GLP-1 SGLT1 GLUT2 TAS1R1 TAS1R3 |
| title | Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker rat |
| title_full | Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker rat |
| title_fullStr | Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker rat |
| title_full_unstemmed | Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker rat |
| title_short | Roux-en-Y gastric bypass alters intestinal glucose transport in the obese Zucker rat |
| title_sort | roux en y gastric bypass alters intestinal glucose transport in the obese zucker rat |
| topic | RYGB GLP-1 SGLT1 GLUT2 TAS1R1 TAS1R3 |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.901984/full |
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