CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO
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Universidade Federal de Rondonópolis
2017-08-01
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| author | L. B. Lopes C. Eckstein V. S. Moustacas M. F. d’Ornellas H. L. S. Ponce |
| author_facet | L. B. Lopes C. Eckstein V. S. Moustacas M. F. d’Ornellas H. L. S. Ponce |
| author_sort | L. B. Lopes |
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CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO
L. B. Lopes, C. Eckstein, V. S. Moustacas, M. F. d’Ornellas, H. L. S. Ponce
RESUMO
Brucellosis is a zoonotic infectious disease is a chronic condition caused by bacteria of the genus Brucella, which affect animals and man. The disease has become endemic in Brazil, resulting in economic losses both from the point of view of beef production and public health. This study aims to demonstrate the occurrence of animal reagents based on card test (CT), comparing the results with the occurrence of gross lesions at post - slaughter and identification of bacterial DNA by the technique of chain reaction by (PCR). Blood samples were obtained immediately after animals stunning were also collected and the lymph nodes in the slaughter lines. Associated with the collection of material for laboratory diagnosis, made up the inspection for macroscopic lesions, following the routine of the Federal Inspection Service (SIF) of the cattle slaughterhouse located in the municipality of Colíder/MT. In total, 622 blood samples were collected, 14.3% were positive according to the test of CT. According to Kappa test, there is a poor correlation entity tests. Based on the McNemar test, the occurrence of the results is significantly different between the two tests. |
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| spelling | doaj.art-6133e7c984364f45a17f1a6b53a639992022-12-22T01:29:25ZengUniversidade Federal de RondonópolisScientific Electronic Archives2316-92812316-92812017-08-011041319CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSOL. B. LopesC. EcksteinV. S. MoustacasM. F. d’OrnellasH. L. S. PonceLogo do cabeçalho da página SCIENTIFIC ELECTRONIC ARCHIVES OPEN JOURNAL SYSTEMS Ajuda do sistema USUÁRIO Logado como: berber Perfil Sair do sistema NOTIFICAÇÕES Visualizar (115 nova(s)) Gerenciar IDIOMA Selecione o idiomaSubmeter CONTEÚDO DA REVISTA Pesquisa Escopo da Busca Pesquisar Procurar Por Edição Por Autor Por título TAMANHO DE FONTE Make font size smallerMake font size defaultMake font size larger INFORMAÇÕES Para leitores Para Autores Para Bibliotecários CAPA SOBRE PÁGINA DO USUÁRIO PESQUISA ATUAL ANTERIORES NOTÍCIAS Capa > v. 10, n. 4 (2017) > Lopes CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO L. B. Lopes, C. Eckstein, V. S. Moustacas, M. F. d’Ornellas, H. L. S. Ponce RESUMO Brucellosis is a zoonotic infectious disease is a chronic condition caused by bacteria of the genus Brucella, which affect animals and man. The disease has become endemic in Brazil, resulting in economic losses both from the point of view of beef production and public health. This study aims to demonstrate the occurrence of animal reagents based on card test (CT), comparing the results with the occurrence of gross lesions at post - slaughter and identification of bacterial DNA by the technique of chain reaction by (PCR). Blood samples were obtained immediately after animals stunning were also collected and the lymph nodes in the slaughter lines. Associated with the collection of material for laboratory diagnosis, made up the inspection for macroscopic lesions, following the routine of the Federal Inspection Service (SIF) of the cattle slaughterhouse located in the municipality of Colíder/MT. In total, 622 blood samples were collected, 14.3% were positive according to the test of CT. According to Kappa test, there is a poor correlation entity tests. Based on the McNemar test, the occurrence of the results is significantly different between the two tests.http://www.seasinop.com.br/revista/index.php?journal=SEA&page=article&op=view&path%5B%5D=450&path%5B%5D=pdfBrucellosisepidemiologycattle |
| spellingShingle | L. B. Lopes C. Eckstein V. S. Moustacas M. F. d’Ornellas H. L. S. Ponce CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO Scientific Electronic Archives Brucellosis epidemiology cattle |
| title | CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO |
| title_full | CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO |
| title_fullStr | CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO |
| title_full_unstemmed | CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO |
| title_short | CARD TEST AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN CULLED ANIMALS IN A SLAUGHTERHOUSE AT MATO GROSSO |
| title_sort | card test and polymerase chain reaction for the diagnosis of bovine brucellosis in culled animals in a slaughterhouse at mato grosso |
| topic | Brucellosis epidemiology cattle |
| url | http://www.seasinop.com.br/revista/index.php?journal=SEA&page=article&op=view&path%5B%5D=450&path%5B%5D=pdf |
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