Three-dimensional modelling of blur property for conventional optical microscopes
Intensity diffusion caused by optical diffraction limits the imaging resolution of conventional optical microscopes, therefore modelling and measuring the intensity transmission and distribution property of the light sources is a significant research topic in system development and pattern recogniti...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-07-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023050776 |
| _version_ | 1797771496760803328 |
|---|---|
| author | Weihan Hou Yangjie Wei |
| author_facet | Weihan Hou Yangjie Wei |
| author_sort | Weihan Hou |
| collection | DOAJ |
| description | Intensity diffusion caused by optical diffraction limits the imaging resolution of conventional optical microscopes, therefore modelling and measuring the intensity transmission and distribution property of the light sources is a significant research topic in system development and pattern recognition. However, the complicated wave propagation process in optical imaging makes it difficult to provide a direct, analytical and simple mathematical model to measure the relationship between the blur degree and various camera parameters. In this study, an improved intensity transmission and distribution calculation method for conventional optical microscopes was proposed; furthermore, a simple mathematical relation between the blur degree and camera parameters was achieved based on the proposed method. First, the light intensity distribution and propagation characteristics of a conventional optical microscope were modeled based on the property of the Fresnel circular hole diffraction combined with the practical optical parameters. Second, by analyzing the property of intensity distribution and blurring imaging, a quantitative simplified mathematical relationship between the blur degree and camera parameters in optical microscope imaging was obtained, and the three-dimensional (3D) blur property in the optical imaging process was analyzed under different conditions. Third, the connection between diffractive optics and geometric optics was obtained by summarizing and generalizing the 3D blur property curve of each monochromatic light source. Finally, the proposed method was verified through a series of simulations and experiments. |
| first_indexed | 2024-03-12T21:37:28Z |
| format | Article |
| id | doaj.art-61342574ddea4da686be746f30bdc784 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T21:37:28Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-61342574ddea4da686be746f30bdc7842023-07-27T05:57:44ZengElsevierHeliyon2405-84402023-07-0197e17869Three-dimensional modelling of blur property for conventional optical microscopesWeihan Hou0Yangjie Wei1Key Laboratory of Intelligent Computing in Medical Image, Ministry of Education, College of Computer Science and Engineering, Northeastern University, Wenhua Street 3, Shenyang 110819, ChinaCorresponding author.; Key Laboratory of Intelligent Computing in Medical Image, Ministry of Education, College of Computer Science and Engineering, Northeastern University, Wenhua Street 3, Shenyang 110819, ChinaIntensity diffusion caused by optical diffraction limits the imaging resolution of conventional optical microscopes, therefore modelling and measuring the intensity transmission and distribution property of the light sources is a significant research topic in system development and pattern recognition. However, the complicated wave propagation process in optical imaging makes it difficult to provide a direct, analytical and simple mathematical model to measure the relationship between the blur degree and various camera parameters. In this study, an improved intensity transmission and distribution calculation method for conventional optical microscopes was proposed; furthermore, a simple mathematical relation between the blur degree and camera parameters was achieved based on the proposed method. First, the light intensity distribution and propagation characteristics of a conventional optical microscope were modeled based on the property of the Fresnel circular hole diffraction combined with the practical optical parameters. Second, by analyzing the property of intensity distribution and blurring imaging, a quantitative simplified mathematical relationship between the blur degree and camera parameters in optical microscope imaging was obtained, and the three-dimensional (3D) blur property in the optical imaging process was analyzed under different conditions. Third, the connection between diffractive optics and geometric optics was obtained by summarizing and generalizing the 3D blur property curve of each monochromatic light source. Finally, the proposed method was verified through a series of simulations and experiments.http://www.sciencedirect.com/science/article/pii/S2405844023050776Blur propertyOptical microscopeOptical diffractionBlur degree |
| spellingShingle | Weihan Hou Yangjie Wei Three-dimensional modelling of blur property for conventional optical microscopes Heliyon Blur property Optical microscope Optical diffraction Blur degree |
| title | Three-dimensional modelling of blur property for conventional optical microscopes |
| title_full | Three-dimensional modelling of blur property for conventional optical microscopes |
| title_fullStr | Three-dimensional modelling of blur property for conventional optical microscopes |
| title_full_unstemmed | Three-dimensional modelling of blur property for conventional optical microscopes |
| title_short | Three-dimensional modelling of blur property for conventional optical microscopes |
| title_sort | three dimensional modelling of blur property for conventional optical microscopes |
| topic | Blur property Optical microscope Optical diffraction Blur degree |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023050776 |
| work_keys_str_mv | AT weihanhou threedimensionalmodellingofblurpropertyforconventionalopticalmicroscopes AT yangjiewei threedimensionalmodellingofblurpropertyforconventionalopticalmicroscopes |