Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plant
Moringa oleifera (or the miracle tree) is a wild plant species widely grown for its seed pods and leaves, and is used in traditional herbal medicine. The metagenomic whole genome shotgun sequencing (mWGS) approach was used to characterize antibiotic resistance genes (ARGs) of the rhizobiomes of this...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.990169/full |
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| author | Ashwag Y. Shami Aala A. Abulfaraj Mohammed Y. Refai Aminah A. Barqawi Najat Binothman Manal A. Tashkandi Hanadi M. Baeissa Lina Baz Haneen W. Abuauf Ruba A. Ashy Rewaa S. Jalal |
| author_facet | Ashwag Y. Shami Aala A. Abulfaraj Mohammed Y. Refai Aminah A. Barqawi Najat Binothman Manal A. Tashkandi Hanadi M. Baeissa Lina Baz Haneen W. Abuauf Ruba A. Ashy Rewaa S. Jalal |
| author_sort | Ashwag Y. Shami |
| collection | DOAJ |
| description | Moringa oleifera (or the miracle tree) is a wild plant species widely grown for its seed pods and leaves, and is used in traditional herbal medicine. The metagenomic whole genome shotgun sequencing (mWGS) approach was used to characterize antibiotic resistance genes (ARGs) of the rhizobiomes of this wild plant and surrounding bulk soil microbiomes and to figure out the chance and consequences for highly abundant ARGs, e.g., mtrA, golS, soxR, oleC, novA, kdpE, vanRO, parY, and rbpA, to horizontally transfer to human gut pathogens via mobile genetic elements (MGEs). The results indicated that abundance of these ARGs, except for golS, was higher in rhizosphere of M. oleifera than that in bulk soil microbiome with no signs of emerging new soil ARGs in either soil type. The most highly abundant metabolic processes of the most abundant ARGs were previously detected in members of phyla Actinobacteria, Proteobacteria, Acidobacteria, Chloroflexi, and Firmicutes. These processes refer to three resistance mechanisms namely antibiotic efflux pump, antibiotic target alteration and antibiotic target protection. Antibiotic efflux mechanism included resistance-nodulation-cell division (RND), ATP-binding cassette (ABC), and major facilitator superfamily (MFS) antibiotics pumps as well as the two-component regulatory kdpDE system. Antibiotic target alteration included glycopeptide resistance gene cluster (vanRO), aminocoumarin resistance parY, and aminocoumarin self-resistance parY. While, antibiotic target protection mechanism included RbpA bacterial RNA polymerase (rpoB)-binding protein. The study supports the claim of the possible horizontal transfer of these ARGs to human gut and emergence of new multidrug resistant clinical isolates. Thus, careful agricultural practices are required especially for plants used in circles of human nutrition industry or in traditional medicine. |
| first_indexed | 2024-04-11T09:56:16Z |
| format | Article |
| id | doaj.art-6134aabe7c86459aa5e9226448e1ffad |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T09:56:16Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6134aabe7c86459aa5e9226448e1ffad2022-12-22T04:30:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.990169990169Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plantAshwag Y. Shami0Aala A. Abulfaraj1Mohammed Y. Refai2Aminah A. Barqawi3Najat Binothman4Manal A. Tashkandi5Hanadi M. Baeissa6Lina Baz7Haneen W. Abuauf8Ruba A. Ashy9Rewaa S. Jalal10Department of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, Riyadh 11617, Saudi ArabiaBiological Sciences Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi ArabiaDepartment of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi ArabiaDepartment of Chemistry, Al-Leith University College, Umm Al Qura University, Makkah, Saudi ArabiaDepartment of Chemistry, College of Sciences and Arts, King Abdulaziz University, Rabigh, Saudi ArabiaDepartment of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi ArabiaDepartment of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi ArabiaDepartment of Biochemistry, Faculty of Science—King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Biology, College of Science, University of Jeddah, Jeddah, Saudi ArabiaDepartment of Biology, College of Science, University of Jeddah, Jeddah, Saudi ArabiaMoringa oleifera (or the miracle tree) is a wild plant species widely grown for its seed pods and leaves, and is used in traditional herbal medicine. The metagenomic whole genome shotgun sequencing (mWGS) approach was used to characterize antibiotic resistance genes (ARGs) of the rhizobiomes of this wild plant and surrounding bulk soil microbiomes and to figure out the chance and consequences for highly abundant ARGs, e.g., mtrA, golS, soxR, oleC, novA, kdpE, vanRO, parY, and rbpA, to horizontally transfer to human gut pathogens via mobile genetic elements (MGEs). The results indicated that abundance of these ARGs, except for golS, was higher in rhizosphere of M. oleifera than that in bulk soil microbiome with no signs of emerging new soil ARGs in either soil type. The most highly abundant metabolic processes of the most abundant ARGs were previously detected in members of phyla Actinobacteria, Proteobacteria, Acidobacteria, Chloroflexi, and Firmicutes. These processes refer to three resistance mechanisms namely antibiotic efflux pump, antibiotic target alteration and antibiotic target protection. Antibiotic efflux mechanism included resistance-nodulation-cell division (RND), ATP-binding cassette (ABC), and major facilitator superfamily (MFS) antibiotics pumps as well as the two-component regulatory kdpDE system. Antibiotic target alteration included glycopeptide resistance gene cluster (vanRO), aminocoumarin resistance parY, and aminocoumarin self-resistance parY. While, antibiotic target protection mechanism included RbpA bacterial RNA polymerase (rpoB)-binding protein. The study supports the claim of the possible horizontal transfer of these ARGs to human gut and emergence of new multidrug resistant clinical isolates. Thus, careful agricultural practices are required especially for plants used in circles of human nutrition industry or in traditional medicine.https://www.frontiersin.org/articles/10.3389/fmicb.2022.990169/fullresistomehorizontal gene transferantibiotic targetrhizospheremicrobiomeefflux pump |
| spellingShingle | Ashwag Y. Shami Aala A. Abulfaraj Mohammed Y. Refai Aminah A. Barqawi Najat Binothman Manal A. Tashkandi Hanadi M. Baeissa Lina Baz Haneen W. Abuauf Ruba A. Ashy Rewaa S. Jalal Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plant Frontiers in Microbiology resistome horizontal gene transfer antibiotic target rhizosphere microbiome efflux pump |
| title | Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plant |
| title_full | Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plant |
| title_fullStr | Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plant |
| title_full_unstemmed | Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plant |
| title_short | Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plant |
| title_sort | abundant antibiotic resistance genes in rhizobiome of the human edible moringa oleifera medicinal plant |
| topic | resistome horizontal gene transfer antibiotic target rhizosphere microbiome efflux pump |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.990169/full |
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