Evaluation of O<sub>3</sub> Effects on Cumulative Photosynthetic CO<sub>2</sub> Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O<sub>3</sub> Uptake
The current level of tropospheric ozone (O<sub>3</sub>) is expected to reduce the net primary production of forest trees. Here, we evaluated the negative effects of O<sub>3</sub> on the photosynthetic CO<sub>2</sub> uptake of Japanese forest trees species based on...
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MDPI AG
2019-07-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/10/7/556 |
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| author | Masahiro Yamaguchi Yoshiyuki Kinose Hideyuki Matsumura Takeshi Izuta |
| author_facet | Masahiro Yamaguchi Yoshiyuki Kinose Hideyuki Matsumura Takeshi Izuta |
| author_sort | Masahiro Yamaguchi |
| collection | DOAJ |
| description | The current level of tropospheric ozone (O<sub>3</sub>) is expected to reduce the net primary production of forest trees. Here, we evaluated the negative effects of O<sub>3</sub> on the photosynthetic CO<sub>2</sub> uptake of Japanese forest trees species based on their cumulative stomatal O<sub>3</sub> uptake, defined as the phytotoxic O<sub>3</sub> dose (POD). Seedlings of four representative Japanese deciduous broad-leaved forest tree species (<i>Fagus crenata</i>, <i>Quercus serrata</i>, <i>Quercus mongolica</i> var. <i>crispula</i> and <i>Betula platyphylla</i> var. <i>japonica</i>) were exposed to different O<sub>3</sub> concentrations in open-top chambers for two growing seasons. The photosynthesis−light response curves (<i>A</i>-light curves) and stomatal conductance were measured to estimate the leaf-level cumulative photosynthetic CO<sub>2</sub> uptake (Σ<i>P</i><sub>n_est</sub>) and POD, respectively. The whole-plant-level Σ<i>P</i><sub>n_est</sub> were highly correlated with the whole-plant dry mass increments over the two growing seasons. Because whole-plant growth is largely determined by the amount of leaf area per plant and net photosynthetic rate per leaf area, this result suggests that leaf-level Σ<i>P</i><sub>n_est</sub>, which was estimated from the monthly <i>A</i>-light curves and hourly PPFD, could reflect the cumulative photosynthetic CO<sub>2</sub> uptake of the seedlings per unit leaf area. Although the O<sub>3</sub>-induced reductions in the leaf-level Σ<i>P</i><sub>n_est</sub> were well explained by POD in all four tree species, species-specific responses of leaf-level Σ<i>P</i><sub>n_est</sub> to POD were observed. In addition, the flux threshold appropriate for the linear regression of the responses of relative leaf-level Σ<i>P</i><sub>n_est</sub> to POD was also species-specific. Therefore, species-specific responses of cumulative photosynthetic CO<sub>2</sub> uptake to POD could be used to accurately evaluate O<sub>3</sub> impact on the net primary production of deciduous broad-leaved trees. |
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| spelling | doaj.art-613509dd0f9648928971066acb6655dc2022-12-22T03:08:13ZengMDPI AGForests1999-49072019-07-0110755610.3390/f10070556f10070556Evaluation of O<sub>3</sub> Effects on Cumulative Photosynthetic CO<sub>2</sub> Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O<sub>3</sub> UptakeMasahiro Yamaguchi0Yoshiyuki Kinose1Hideyuki Matsumura2Takeshi Izuta3Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, JapanUnited Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, JapanEnvironmental Science Research Laboratory, Central Research Institute of Electric Power Industry, Abiko, Chiba 270-1194, JapanInstitute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, JapanThe current level of tropospheric ozone (O<sub>3</sub>) is expected to reduce the net primary production of forest trees. Here, we evaluated the negative effects of O<sub>3</sub> on the photosynthetic CO<sub>2</sub> uptake of Japanese forest trees species based on their cumulative stomatal O<sub>3</sub> uptake, defined as the phytotoxic O<sub>3</sub> dose (POD). Seedlings of four representative Japanese deciduous broad-leaved forest tree species (<i>Fagus crenata</i>, <i>Quercus serrata</i>, <i>Quercus mongolica</i> var. <i>crispula</i> and <i>Betula platyphylla</i> var. <i>japonica</i>) were exposed to different O<sub>3</sub> concentrations in open-top chambers for two growing seasons. The photosynthesis−light response curves (<i>A</i>-light curves) and stomatal conductance were measured to estimate the leaf-level cumulative photosynthetic CO<sub>2</sub> uptake (Σ<i>P</i><sub>n_est</sub>) and POD, respectively. The whole-plant-level Σ<i>P</i><sub>n_est</sub> were highly correlated with the whole-plant dry mass increments over the two growing seasons. Because whole-plant growth is largely determined by the amount of leaf area per plant and net photosynthetic rate per leaf area, this result suggests that leaf-level Σ<i>P</i><sub>n_est</sub>, which was estimated from the monthly <i>A</i>-light curves and hourly PPFD, could reflect the cumulative photosynthetic CO<sub>2</sub> uptake of the seedlings per unit leaf area. Although the O<sub>3</sub>-induced reductions in the leaf-level Σ<i>P</i><sub>n_est</sub> were well explained by POD in all four tree species, species-specific responses of leaf-level Σ<i>P</i><sub>n_est</sub> to POD were observed. In addition, the flux threshold appropriate for the linear regression of the responses of relative leaf-level Σ<i>P</i><sub>n_est</sub> to POD was also species-specific. Therefore, species-specific responses of cumulative photosynthetic CO<sub>2</sub> uptake to POD could be used to accurately evaluate O<sub>3</sub> impact on the net primary production of deciduous broad-leaved trees.https://www.mdpi.com/1999-4907/10/7/556Japanese deciduous broad-leaved treesnet photosynthetic ratenet primary productionstomatal O<sub>3</sub> uptakephytotoxic O<sub>3</sub> dose |
| spellingShingle | Masahiro Yamaguchi Yoshiyuki Kinose Hideyuki Matsumura Takeshi Izuta Evaluation of O<sub>3</sub> Effects on Cumulative Photosynthetic CO<sub>2</sub> Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O<sub>3</sub> Uptake Forests Japanese deciduous broad-leaved trees net photosynthetic rate net primary production stomatal O<sub>3</sub> uptake phytotoxic O<sub>3</sub> dose |
| title | Evaluation of O<sub>3</sub> Effects on Cumulative Photosynthetic CO<sub>2</sub> Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O<sub>3</sub> Uptake |
| title_full | Evaluation of O<sub>3</sub> Effects on Cumulative Photosynthetic CO<sub>2</sub> Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O<sub>3</sub> Uptake |
| title_fullStr | Evaluation of O<sub>3</sub> Effects on Cumulative Photosynthetic CO<sub>2</sub> Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O<sub>3</sub> Uptake |
| title_full_unstemmed | Evaluation of O<sub>3</sub> Effects on Cumulative Photosynthetic CO<sub>2</sub> Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O<sub>3</sub> Uptake |
| title_short | Evaluation of O<sub>3</sub> Effects on Cumulative Photosynthetic CO<sub>2</sub> Uptake in Seedlings of Four Japanese Deciduous Broad-Leaved Forest Tree Species Based on Stomatal O<sub>3</sub> Uptake |
| title_sort | evaluation of o sub 3 sub effects on cumulative photosynthetic co sub 2 sub uptake in seedlings of four japanese deciduous broad leaved forest tree species based on stomatal o sub 3 sub uptake |
| topic | Japanese deciduous broad-leaved trees net photosynthetic rate net primary production stomatal O<sub>3</sub> uptake phytotoxic O<sub>3</sub> dose |
| url | https://www.mdpi.com/1999-4907/10/7/556 |
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