Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of <i>Enterococcus faecalis</i> by PUI and XPF File Activation
Background: Biofilm removal from the root canal during endodontic treatment is necessary to prevent further complications. Irrigation is essential to success. Several irrigants have been proposed without a proper comparison. The aim of the study is to compare the antibacterial capacity of different...
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MDPI AG
2021-04-01
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| Series: | Dentistry Journal |
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| Online Access: | https://www.mdpi.com/2304-6767/9/4/41 |
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| author | Alejandro Álvarez-Sagües Nerea Herce Ulises Amador Francisco Llinares-Pinel Estanislao Nistal-Villan Jesús Presa Laura Álvarez Magdalena Azabal |
| author_facet | Alejandro Álvarez-Sagües Nerea Herce Ulises Amador Francisco Llinares-Pinel Estanislao Nistal-Villan Jesús Presa Laura Álvarez Magdalena Azabal |
| author_sort | Alejandro Álvarez-Sagües |
| collection | DOAJ |
| description | Background: Biofilm removal from the root canal during endodontic treatment is necessary to prevent further complications. Irrigation is essential to success. Several irrigants have been proposed without a proper comparison. The aim of the study is to compare the antibacterial capacity of different activated irrigants using passive ultrasonic activation (PUI) or XP-Endo finisher (XPF). Methods: A total of 100 instrumented teeth were incubated in an Eppendorf tube containing 0.5 McFarland of <i>Enterococcus faecalis</i> and incubated for 2 weeks at 37 °C. Roots were divided into 5 groups (<i>n</i> = 20) according to the irrigant type: ethylenediaminetetraacetic acid (EDTA) (17%), ethydronic acid (HEDP) (9%) mixed with 5.25% sodium hypochlorite (NaOCl), EDTA (17%) mixed with 5.25% NaOCl, PBS, and a control group. Each group was divided into two subgroups (<i>n</i> = 10): PUI and XPF. Results: As compared to the untreated control group, the irrigators included in the study had a significant effect in bacteria reduction. The obtained results show HEDP to be the most effective irrigant, since no bacteria were recovered after treatment of this group, followed by EDTA mixed with NaOCl and, finally, the EDTA-irrigated group. Conclusions: HEDP is the best irrigating agent in combination with XPF or PUI file activation to eliminate bacteria in our experimental model. |
| first_indexed | 2024-03-10T12:27:57Z |
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| language | English |
| last_indexed | 2024-03-10T12:27:57Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-6136b0e042bb43a2b9d96c35f4c808732023-11-21T14:53:50ZengMDPI AGDentistry Journal2304-67672021-04-01944110.3390/dj9040041Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of <i>Enterococcus faecalis</i> by PUI and XPF File ActivationAlejandro Álvarez-Sagües0Nerea Herce1Ulises Amador2Francisco Llinares-Pinel3Estanislao Nistal-Villan4Jesús Presa5Laura Álvarez6Magdalena Azabal7Dentistry Department, Faculty of Medicine, San Pablo CEU University, 28668 Madrid, SpainDentistry Department, Faculty of Medicine, San Pablo CEU University, 28668 Madrid, SpainDepartamento de Química y Bioquímica, Facultad de Farmacia, Universidad San Pablo-CEU, Urbanización Montepríncipe, Boadilla del Monte, 28668 Madrid, SpainMicrobiology Section, Departamento CC, Farmacéuticas y de la Salud, Facultad de Farmacia, Universidad CEU San Pablo, Boadilla del Monte, 28668 Madrid, SpainMicrobiology Section, Departamento CC, Farmacéuticas y de la Salud, Facultad de Farmacia, Universidad CEU San Pablo, Boadilla del Monte, 28668 Madrid, SpainIndependent Researcher, 28668 Madrid, SpainDentistry Department, Faculty of Medicine, San Pablo CEU University, 28668 Madrid, SpainDentistry Department, Faculty of Medicine, San Pablo CEU University, 28668 Madrid, SpainBackground: Biofilm removal from the root canal during endodontic treatment is necessary to prevent further complications. Irrigation is essential to success. Several irrigants have been proposed without a proper comparison. The aim of the study is to compare the antibacterial capacity of different activated irrigants using passive ultrasonic activation (PUI) or XP-Endo finisher (XPF). Methods: A total of 100 instrumented teeth were incubated in an Eppendorf tube containing 0.5 McFarland of <i>Enterococcus faecalis</i> and incubated for 2 weeks at 37 °C. Roots were divided into 5 groups (<i>n</i> = 20) according to the irrigant type: ethylenediaminetetraacetic acid (EDTA) (17%), ethydronic acid (HEDP) (9%) mixed with 5.25% sodium hypochlorite (NaOCl), EDTA (17%) mixed with 5.25% NaOCl, PBS, and a control group. Each group was divided into two subgroups (<i>n</i> = 10): PUI and XPF. Results: As compared to the untreated control group, the irrigators included in the study had a significant effect in bacteria reduction. The obtained results show HEDP to be the most effective irrigant, since no bacteria were recovered after treatment of this group, followed by EDTA mixed with NaOCl and, finally, the EDTA-irrigated group. Conclusions: HEDP is the best irrigating agent in combination with XPF or PUI file activation to eliminate bacteria in our experimental model.https://www.mdpi.com/2304-6767/9/4/41<i>Enterococcus faecalis</i>EDTAethydronic acid (HEDP)passive ultrasonic activation (PUI)Endo-XP finisher (XPF) |
| spellingShingle | Alejandro Álvarez-Sagües Nerea Herce Ulises Amador Francisco Llinares-Pinel Estanislao Nistal-Villan Jesús Presa Laura Álvarez Magdalena Azabal Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of <i>Enterococcus faecalis</i> by PUI and XPF File Activation Dentistry Journal <i>Enterococcus faecalis</i> EDTA ethydronic acid (HEDP) passive ultrasonic activation (PUI) Endo-XP finisher (XPF) |
| title | Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of <i>Enterococcus faecalis</i> by PUI and XPF File Activation |
| title_full | Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of <i>Enterococcus faecalis</i> by PUI and XPF File Activation |
| title_fullStr | Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of <i>Enterococcus faecalis</i> by PUI and XPF File Activation |
| title_full_unstemmed | Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of <i>Enterococcus faecalis</i> by PUI and XPF File Activation |
| title_short | Efficacy of EDTA and HEDP Chelators in the Removal of Mature Biofilm of <i>Enterococcus faecalis</i> by PUI and XPF File Activation |
| title_sort | efficacy of edta and hedp chelators in the removal of mature biofilm of i enterococcus faecalis i by pui and xpf file activation |
| topic | <i>Enterococcus faecalis</i> EDTA ethydronic acid (HEDP) passive ultrasonic activation (PUI) Endo-XP finisher (XPF) |
| url | https://www.mdpi.com/2304-6767/9/4/41 |
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