Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae.
The economic production of cellulosic biofuel requires efficient and full utilization of all abundant carbohydrates naturally released from plant biomass by enzyme cocktails. Recently, we reconstituted the Neurospora crassa xylodextrin transport and consumption system in Saccharomyces cerevisiae, en...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5591001?pdf=render |
| _version_ | 1811260294758924288 |
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| author | Chenlu Zhang Ligia Acosta-Sampson Vivian Yaci Yu Jamie H D Cate |
| author_facet | Chenlu Zhang Ligia Acosta-Sampson Vivian Yaci Yu Jamie H D Cate |
| author_sort | Chenlu Zhang |
| collection | DOAJ |
| description | The economic production of cellulosic biofuel requires efficient and full utilization of all abundant carbohydrates naturally released from plant biomass by enzyme cocktails. Recently, we reconstituted the Neurospora crassa xylodextrin transport and consumption system in Saccharomyces cerevisiae, enabling growth of yeast on xylodextrins aerobically. However, the consumption rate of xylodextrin requires improvement for industrial applications, including consumption in anaerobic conditions. As a first step in this improvement, we report analysis of orthologues of the N. crassa transporters CDT-1 and CDT-2. Transporter ST16 from Trichoderma virens enables faster aerobic growth of S. cerevisiae on xylodextrins compared to CDT-2. ST16 is a xylodextrin-specific transporter, and the xylobiose transport activity of ST16 is not inhibited by cellobiose. Other transporters identified in the screen also enable growth on xylodextrins including xylotriose. Taken together, these results indicate that multiple transporters might prove useful to improve xylodextrin utilization in S. cerevisiae. Efforts to use directed evolution to improve ST16 from a chromosomally-integrated copy were not successful, due to background growth of yeast on other carbon sources present in the selection medium. Future experiments will require increasing the baseline growth rate of the yeast population on xylodextrins, to ensure that the selective pressure exerted on xylodextrin transport can lead to isolation of improved xylodextrin transporters. |
| first_indexed | 2024-04-12T18:44:16Z |
| format | Article |
| id | doaj.art-613a1f2eaecd412ca70fbf997ec21636 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T18:44:16Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-613a1f2eaecd412ca70fbf997ec216362022-12-22T03:20:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018473010.1371/journal.pone.0184730Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae.Chenlu ZhangLigia Acosta-SampsonVivian Yaci YuJamie H D CateThe economic production of cellulosic biofuel requires efficient and full utilization of all abundant carbohydrates naturally released from plant biomass by enzyme cocktails. Recently, we reconstituted the Neurospora crassa xylodextrin transport and consumption system in Saccharomyces cerevisiae, enabling growth of yeast on xylodextrins aerobically. However, the consumption rate of xylodextrin requires improvement for industrial applications, including consumption in anaerobic conditions. As a first step in this improvement, we report analysis of orthologues of the N. crassa transporters CDT-1 and CDT-2. Transporter ST16 from Trichoderma virens enables faster aerobic growth of S. cerevisiae on xylodextrins compared to CDT-2. ST16 is a xylodextrin-specific transporter, and the xylobiose transport activity of ST16 is not inhibited by cellobiose. Other transporters identified in the screen also enable growth on xylodextrins including xylotriose. Taken together, these results indicate that multiple transporters might prove useful to improve xylodextrin utilization in S. cerevisiae. Efforts to use directed evolution to improve ST16 from a chromosomally-integrated copy were not successful, due to background growth of yeast on other carbon sources present in the selection medium. Future experiments will require increasing the baseline growth rate of the yeast population on xylodextrins, to ensure that the selective pressure exerted on xylodextrin transport can lead to isolation of improved xylodextrin transporters.http://europepmc.org/articles/PMC5591001?pdf=render |
| spellingShingle | Chenlu Zhang Ligia Acosta-Sampson Vivian Yaci Yu Jamie H D Cate Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae. PLoS ONE |
| title | Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae. |
| title_full | Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae. |
| title_fullStr | Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae. |
| title_full_unstemmed | Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae. |
| title_short | Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae. |
| title_sort | screening of transporters to improve xylodextrin utilization in the yeast saccharomyces cerevisiae |
| url | http://europepmc.org/articles/PMC5591001?pdf=render |
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