Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model
Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2016-06-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/445603 |
| _version_ | 1818573845801467904 |
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| author | Guangbing Wei Xin Chen Guanghui Wang Lin Fan Kang Wang Xuqi Li |
| author_facet | Guangbing Wei Xin Chen Guanghui Wang Lin Fan Kang Wang Xuqi Li |
| author_sort | Guangbing Wei |
| collection | DOAJ |
| description | Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value. |
| first_indexed | 2024-12-15T00:18:21Z |
| format | Article |
| id | doaj.art-613cd039e5e64ef79525f48149616917 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-15T00:18:21Z |
| publishDate | 2016-06-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-613cd039e5e64ef79525f481496169172022-12-21T22:42:24ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-06-01391334610.1159/000445603445603Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat ModelGuangbing WeiXin ChenGuanghui WangLin FanKang WangXuqi LiBackground: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.http://www.karger.com/Article/FullText/445603Postoperative adhesionsResveratrolTGF-β1IL-6Inflammatory Response |
| spellingShingle | Guangbing Wei Xin Chen Guanghui Wang Lin Fan Kang Wang Xuqi Li Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model Cellular Physiology and Biochemistry Postoperative adhesions Resveratrol TGF-β1 IL-6 Inflammatory Response |
| title | Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model |
| title_full | Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model |
| title_fullStr | Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model |
| title_full_unstemmed | Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model |
| title_short | Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model |
| title_sort | effect of resveratrol on the prevention of intra abdominal adhesion formation in a rat model |
| topic | Postoperative adhesions Resveratrol TGF-β1 IL-6 Inflammatory Response |
| url | http://www.karger.com/Article/FullText/445603 |
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