Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice
Abstract The sebaceous gland (SG) is involved in different inflammatory, infectious and neoplastic processes of the skin and can be related to specific diseases, e.g., diabetes mellitus. Sometimes, the histological diagnosis requires complementary tests due to the ability of diseases to mimic other...
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| Format: | Article |
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Nature Portfolio
2021-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-91459-x |
| _version_ | 1818435123616415744 |
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| author | Geovane Ribeiro Santos Marcelo Rodrigues Cunha Eduardo José Caldeira Ewerton Alexandre Galdeano Raphael Cruz Seabra Prudente Clóvis Antonio Lopes Pinto |
| author_facet | Geovane Ribeiro Santos Marcelo Rodrigues Cunha Eduardo José Caldeira Ewerton Alexandre Galdeano Raphael Cruz Seabra Prudente Clóvis Antonio Lopes Pinto |
| author_sort | Geovane Ribeiro Santos |
| collection | DOAJ |
| description | Abstract The sebaceous gland (SG) is involved in different inflammatory, infectious and neoplastic processes of the skin and can be related to specific diseases, e.g., diabetes mellitus. Sometimes, the histological diagnosis requires complementary tests due to the ability of diseases to mimic other tumors. We evaluated the sebaceous gland density in Non-obese diabetic mice to analyze the N-acetylcystein effects and swimming exercise treatment in sebaceous glands healing, using specific staining in histochemistry and immunohistochemistry reactions in the identification of the lipid expression in the sebaceous gland. We investigated the intracytoplasmic lipid expression and analysis of gland density from SG in dorsal skin samples from the Non-obese diabetic (NOD mice) and diabetic animals submitted to antioxidant treatment and physical exercise. For histological analysis of the sebaceous glands, specific staining in histochemistry with sudan black and immunohistochemistry reaction with adipophilin were used in the evaluation. Statistical analysis showed significant proximity between the values of the control group and the diabetic group submitted to the swimming exercise (DS group) and similar values between the untreated diabetic group (UD group) and diabetic group treated with the antioxidant N-acetylcysteine (DNa group), which did not prevent possible differences where p < 0.01. Adipophilin (ADPH) immunohistochemistry permitted more intense lipid staining in SGs, the preservation of the SG in the control group, and a morphological deformed appearance in the UD and DNa groups. However, weak morphological recovery of the SG was observed in the DS-Na group, being more expressive in the DS group. In conclusion, the groups submitted to physical exercises showed better results in the recovery of the analyzed tissue, even being in the physiological conditions caused by spontaneous diabetes. |
| first_indexed | 2024-12-14T16:47:53Z |
| format | Article |
| id | doaj.art-613ce0dc09bc412a8e6ad2615e3ecb19 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T16:47:53Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-613ce0dc09bc412a8e6ad2615e3ecb192022-12-21T22:54:08ZengNature PortfolioScientific Reports2045-23222021-06-011111910.1038/s41598-021-91459-xEffect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic miceGeovane Ribeiro Santos0Marcelo Rodrigues Cunha1Eduardo José Caldeira2Ewerton Alexandre Galdeano3Raphael Cruz Seabra Prudente4Clóvis Antonio Lopes Pinto5Morphology and Basic Pathology Department, Jundiaí Medical School (JMS) JundiaíMorphology and Basic Pathology Department, Jundiaí Medical School (JMS) JundiaíMorphology and Basic Pathology Department, Jundiaí Medical School (JMS) JundiaíMorphology and Basic Pathology Department, Jundiaí Medical School (JMS) JundiaíHealth Management and Promotion Unit (HMPU)Morphology and Basic Pathology Department, Jundiaí Medical School (JMS) JundiaíAbstract The sebaceous gland (SG) is involved in different inflammatory, infectious and neoplastic processes of the skin and can be related to specific diseases, e.g., diabetes mellitus. Sometimes, the histological diagnosis requires complementary tests due to the ability of diseases to mimic other tumors. We evaluated the sebaceous gland density in Non-obese diabetic mice to analyze the N-acetylcystein effects and swimming exercise treatment in sebaceous glands healing, using specific staining in histochemistry and immunohistochemistry reactions in the identification of the lipid expression in the sebaceous gland. We investigated the intracytoplasmic lipid expression and analysis of gland density from SG in dorsal skin samples from the Non-obese diabetic (NOD mice) and diabetic animals submitted to antioxidant treatment and physical exercise. For histological analysis of the sebaceous glands, specific staining in histochemistry with sudan black and immunohistochemistry reaction with adipophilin were used in the evaluation. Statistical analysis showed significant proximity between the values of the control group and the diabetic group submitted to the swimming exercise (DS group) and similar values between the untreated diabetic group (UD group) and diabetic group treated with the antioxidant N-acetylcysteine (DNa group), which did not prevent possible differences where p < 0.01. Adipophilin (ADPH) immunohistochemistry permitted more intense lipid staining in SGs, the preservation of the SG in the control group, and a morphological deformed appearance in the UD and DNa groups. However, weak morphological recovery of the SG was observed in the DS-Na group, being more expressive in the DS group. In conclusion, the groups submitted to physical exercises showed better results in the recovery of the analyzed tissue, even being in the physiological conditions caused by spontaneous diabetes.https://doi.org/10.1038/s41598-021-91459-x |
| spellingShingle | Geovane Ribeiro Santos Marcelo Rodrigues Cunha Eduardo José Caldeira Ewerton Alexandre Galdeano Raphael Cruz Seabra Prudente Clóvis Antonio Lopes Pinto Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice Scientific Reports |
| title | Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice |
| title_full | Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice |
| title_fullStr | Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice |
| title_full_unstemmed | Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice |
| title_short | Effect of antioxidant treatment with n-acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice |
| title_sort | effect of antioxidant treatment with n acetylcysteine and swimming on lipid expression of sebaceous glands in diabetic mice |
| url | https://doi.org/10.1038/s41598-021-91459-x |
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