Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress
Soil salinity is a major environmental stress that has been negatively affecting the growth and productivity of rice. However, various salt-resistant plant growth-promoting rhizobacteria (PGPR) have been known to promote plant growth and alleviate the damaging effects of salt stress via mitigating p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.994902/full |
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| author | Qurban Ali Muhammad Ayaz Guangyuan Mu Amjad Hussain Qiu Yuanyuan Chenjie Yu Yujiao Xu Hakim Manghwar Qin Gu Huijun Wu Xuewen Gao |
| author_facet | Qurban Ali Muhammad Ayaz Guangyuan Mu Amjad Hussain Qiu Yuanyuan Chenjie Yu Yujiao Xu Hakim Manghwar Qin Gu Huijun Wu Xuewen Gao |
| author_sort | Qurban Ali |
| collection | DOAJ |
| description | Soil salinity is a major environmental stress that has been negatively affecting the growth and productivity of rice. However, various salt-resistant plant growth-promoting rhizobacteria (PGPR) have been known to promote plant growth and alleviate the damaging effects of salt stress via mitigating physio-biochemical and molecular characteristics. This study was conducted to examine the salt stress potential of Bacillus strains identified from harsh environments of the Qinghai-Tibetan plateau region of China. The Bacillus strains NMTD17, GBSW22, and FZB42 were screened for their response under different salt stress conditions (1, 4, 7, 9, 11, 13, and 16%). The screening analysis revealed strains NMTD17, GBSW22, and FZB42 to be high-salt tolerant, moderate-salt tolerant, and salt-sensitive, respectively. The NMTD17 strain produced a strong biofilm, followed by GBSW22 and FZB42. The expression of salt stress-related genes in selected strains was also analyzed through qPCR in various salt concentrations. Further, the Bacillus strains were used in pot experiments to study their growth-promoting ability and antioxidant activities at various concentrations (0, 100, 150, and 200 mmol). The analysis of growth-promoting traits in rice exhibited that NMTD17 had a highly significant effect and GSBW22 had a moderately significant effect in comparison with FZB42. The highly resistant strain NMTD17 that stably promoted rice plant growth was further examined for its function in the composition of rhizobacterial communities. The inoculation of NMTD17 increased the relative abundance and richness of rhizobacterial species. These outcomes propose that NMTD17 possesses the potential of PGPR traits, antioxidants enzyme activities, and reshaping the rhizobacterial community that together mitigate the harmful effects of salinity in rice plants. |
| first_indexed | 2024-04-11T18:35:28Z |
| format | Article |
| id | doaj.art-613e91d32baf49a9afbdfb910779d9b4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T18:35:28Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-613e91d32baf49a9afbdfb910779d9b42022-12-22T04:09:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.994902994902Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stressQurban Ali0Muhammad Ayaz1Guangyuan Mu2Amjad Hussain3Qiu Yuanyuan4Chenjie Yu5Yujiao Xu6Hakim Manghwar7Qin Gu8Huijun Wu9Xuewen Gao10Key Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, The Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, The Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, ChinaShenzhen Batian Ecotypic Engineering Co., Ltd., Shenzhen, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, The Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, The Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, The Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, ChinaLushan Botanical Garden, Chinese Academy of Sciences, Jiujiang, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, The Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, The Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, The Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, ChinaSoil salinity is a major environmental stress that has been negatively affecting the growth and productivity of rice. However, various salt-resistant plant growth-promoting rhizobacteria (PGPR) have been known to promote plant growth and alleviate the damaging effects of salt stress via mitigating physio-biochemical and molecular characteristics. This study was conducted to examine the salt stress potential of Bacillus strains identified from harsh environments of the Qinghai-Tibetan plateau region of China. The Bacillus strains NMTD17, GBSW22, and FZB42 were screened for their response under different salt stress conditions (1, 4, 7, 9, 11, 13, and 16%). The screening analysis revealed strains NMTD17, GBSW22, and FZB42 to be high-salt tolerant, moderate-salt tolerant, and salt-sensitive, respectively. The NMTD17 strain produced a strong biofilm, followed by GBSW22 and FZB42. The expression of salt stress-related genes in selected strains was also analyzed through qPCR in various salt concentrations. Further, the Bacillus strains were used in pot experiments to study their growth-promoting ability and antioxidant activities at various concentrations (0, 100, 150, and 200 mmol). The analysis of growth-promoting traits in rice exhibited that NMTD17 had a highly significant effect and GSBW22 had a moderately significant effect in comparison with FZB42. The highly resistant strain NMTD17 that stably promoted rice plant growth was further examined for its function in the composition of rhizobacterial communities. The inoculation of NMTD17 increased the relative abundance and richness of rhizobacterial species. These outcomes propose that NMTD17 possesses the potential of PGPR traits, antioxidants enzyme activities, and reshaping the rhizobacterial community that together mitigate the harmful effects of salinity in rice plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.994902/fullricecell physiologycellular interactionsantioxidant enzymesPGPRbiofilm |
| spellingShingle | Qurban Ali Muhammad Ayaz Guangyuan Mu Amjad Hussain Qiu Yuanyuan Chenjie Yu Yujiao Xu Hakim Manghwar Qin Gu Huijun Wu Xuewen Gao Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress Frontiers in Plant Science rice cell physiology cellular interactions antioxidant enzymes PGPR biofilm |
| title | Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress |
| title_full | Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress |
| title_fullStr | Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress |
| title_full_unstemmed | Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress |
| title_short | Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress |
| title_sort | revealing plant growth promoting mechanisms of bacillus strains in elevating rice growth and its interaction with salt stress |
| topic | rice cell physiology cellular interactions antioxidant enzymes PGPR biofilm |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.994902/full |
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