Macrophage Identification In Situ
Understanding the processes of inflammation and tissue regeneration after injury is of great importance. For a long time, macrophages have been known to play a central role during different stages of inflammation and tissue regeneration. However, the molecular and cellular mechanisms by which they e...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/10/1393 |
| _version_ | 1797515217812324352 |
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| author | Krisztina Nikovics Anne-Laure Favier |
| author_facet | Krisztina Nikovics Anne-Laure Favier |
| author_sort | Krisztina Nikovics |
| collection | DOAJ |
| description | Understanding the processes of inflammation and tissue regeneration after injury is of great importance. For a long time, macrophages have been known to play a central role during different stages of inflammation and tissue regeneration. However, the molecular and cellular mechanisms by which they exert their effects are as yet mostly unknown. While in vitro macrophages have been characterized, recent progress in macrophage biology studies revealed that macrophages in vivo exhibited distinctive features. Actually, the precise characterization of the macrophages in vivo is essential to develop new healing treatments and can be approached via in situ analyses. Nowadays, the characterization of macrophages in situ has improved significantly using antigen surface markers and cytokine secretion identification resulting in specific patterns. This review aims for a comprehensive overview of different tools used for in situ macrophage identification, reporter genes, immunolabeling and in situ hybridization, discussing their advantages and limitations. |
| first_indexed | 2024-03-10T06:42:24Z |
| format | Article |
| id | doaj.art-613f2e08a91a46c98ae232eaad56745a |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T06:42:24Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-613f2e08a91a46c98ae232eaad56745a2023-11-22T17:31:12ZengMDPI AGBiomedicines2227-90592021-10-01910139310.3390/biomedicines9101393Macrophage Identification In SituKrisztina Nikovics0Anne-Laure Favier1Imagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223 Brétigny-sur-Orge, FranceUnderstanding the processes of inflammation and tissue regeneration after injury is of great importance. For a long time, macrophages have been known to play a central role during different stages of inflammation and tissue regeneration. However, the molecular and cellular mechanisms by which they exert their effects are as yet mostly unknown. While in vitro macrophages have been characterized, recent progress in macrophage biology studies revealed that macrophages in vivo exhibited distinctive features. Actually, the precise characterization of the macrophages in vivo is essential to develop new healing treatments and can be approached via in situ analyses. Nowadays, the characterization of macrophages in situ has improved significantly using antigen surface markers and cytokine secretion identification resulting in specific patterns. This review aims for a comprehensive overview of different tools used for in situ macrophage identification, reporter genes, immunolabeling and in situ hybridization, discussing their advantages and limitations.https://www.mdpi.com/2227-9059/9/10/1393macrophagesphenotypein situ hybridizationcytokinesimmunolabeling |
| spellingShingle | Krisztina Nikovics Anne-Laure Favier Macrophage Identification In Situ Biomedicines macrophages phenotype in situ hybridization cytokines immunolabeling |
| title | Macrophage Identification In Situ |
| title_full | Macrophage Identification In Situ |
| title_fullStr | Macrophage Identification In Situ |
| title_full_unstemmed | Macrophage Identification In Situ |
| title_short | Macrophage Identification In Situ |
| title_sort | macrophage identification in situ |
| topic | macrophages phenotype in situ hybridization cytokines immunolabeling |
| url | https://www.mdpi.com/2227-9059/9/10/1393 |
| work_keys_str_mv | AT krisztinanikovics macrophageidentificationinsitu AT annelaurefavier macrophageidentificationinsitu |