Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric Approach
Essential oils are mainly analyzed by gas chromatography coupled with mass spectrometry (GC–MS). This is a time consuming technique which requires complex instrumentation assembly. Raman and FTIR spectroscopy are rapid and non-destructive techniques for qualitative characterization, differentiation...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722005365 |
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| author | Archasvi Tyagi Anil K. Yadav Akanksha Yadav Lalita Saini Vivek Kumar Pooja Jain Inam Mohammad Mohammad Javed Ansari Hesham Ali El Enshasy Fagr Kh. Abdel-Gawad Sami Al Obaid Shahida Anusha Siddiqui Vijai Malik |
| author_facet | Archasvi Tyagi Anil K. Yadav Akanksha Yadav Lalita Saini Vivek Kumar Pooja Jain Inam Mohammad Mohammad Javed Ansari Hesham Ali El Enshasy Fagr Kh. Abdel-Gawad Sami Al Obaid Shahida Anusha Siddiqui Vijai Malik |
| author_sort | Archasvi Tyagi |
| collection | DOAJ |
| description | Essential oils are mainly analyzed by gas chromatography coupled with mass spectrometry (GC–MS). This is a time consuming technique which requires complex instrumentation assembly. Raman and FTIR spectroscopy are rapid and non-destructive techniques for qualitative characterization, differentiation of principal essential oil components, detection of adulteration in the oil, quality control of essential oil in industry as well as to distinguish different taxa. In the present study, five Ocimum spp. (Ocimum tenuiflorum L., Ocimum gratissimum L., Ocimum × africanum Lour., Ocimum basilicum L. and Ocimum americanum L) have been identified on the basis of essential oil by the Raman and FTIR spectroscopic techniques. It was found that methyl eugenol, eugenol, estragole, and camphor are major essential oils components present in Ocimum L. The results of PCA indicate that all five species of Ocimum L. form different clusters on the basis of principal essential oil components. The components of essential oil predicted by Raman spectroscopy correlate well with that of FTIR data. The present study demonstrates the use of Raman and FTIR spectroscopy techniques to distinguish taxa and characterization of essential oil components. |
| first_indexed | 2024-04-11T23:35:39Z |
| format | Article |
| id | doaj.art-613fbd3be0fe4cf4b2177b24a90f49fd |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-11T23:35:39Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-613fbd3be0fe4cf4b2177b24a90f49fd2022-12-22T03:56:57ZengElsevierJournal of King Saud University: Science1018-36472022-11-01348102355Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric ApproachArchasvi Tyagi0Anil K. Yadav1Akanksha Yadav2Lalita Saini3Vivek Kumar4Pooja Jain5Inam Mohammad6Mohammad Javed Ansari7Hesham Ali El Enshasy8Fagr Kh. Abdel-Gawad9Sami Al Obaid10Shahida Anusha Siddiqui11Vijai Malik12Department of Botany, CCS University, Meerut, Uttar Pradesh 250004, IndiaDepartment of Physics, CCS University, Meerut, Uttar Pradesh 250004, IndiaDepartment of Physics, CCS University, Meerut, Uttar Pradesh 250004, IndiaDepartment of Botany, CCS University, Meerut, Uttar Pradesh 250004, IndiaDepartment of Botany, CCS University, Meerut, Uttar Pradesh 250004, IndiaDepartment of Botany, CCS University, Meerut, Uttar Pradesh 250004, IndiaDepartment of Botany, CCS University, Meerut, Uttar Pradesh 250004, IndiaDepartment of Botany, Hindu College Moradabad (Mahatma Jyotiba Phule Rohilkhand University Bareilly-India), 244001, IndiaInstitute of Bioproduct Development (IBD), Universiti Teknologi Malaysia, Skudai, Johor Bahru, Malaysia; City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria, EgyptEnvironmental Research and Climate Change Institute, National Research Centre (NRC), 33 El Bohouth st. (Former El Tahrir st.), P. O.12622, Dokki, Giza, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh 17-11451, Saudi ArabiaCampus Straubing for Biotechnology and Sustainability, Technical University of Munich, Essigberg 3, 94315 Straubing, Germany; German Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Straße 7, 49610 Quakenbrück, GermanyDepartment of Botany, CCS University, Meerut, Uttar Pradesh 250004, India; Corresponding author.Essential oils are mainly analyzed by gas chromatography coupled with mass spectrometry (GC–MS). This is a time consuming technique which requires complex instrumentation assembly. Raman and FTIR spectroscopy are rapid and non-destructive techniques for qualitative characterization, differentiation of principal essential oil components, detection of adulteration in the oil, quality control of essential oil in industry as well as to distinguish different taxa. In the present study, five Ocimum spp. (Ocimum tenuiflorum L., Ocimum gratissimum L., Ocimum × africanum Lour., Ocimum basilicum L. and Ocimum americanum L) have been identified on the basis of essential oil by the Raman and FTIR spectroscopic techniques. It was found that methyl eugenol, eugenol, estragole, and camphor are major essential oils components present in Ocimum L. The results of PCA indicate that all five species of Ocimum L. form different clusters on the basis of principal essential oil components. The components of essential oil predicted by Raman spectroscopy correlate well with that of FTIR data. The present study demonstrates the use of Raman and FTIR spectroscopy techniques to distinguish taxa and characterization of essential oil components.http://www.sciencedirect.com/science/article/pii/S1018364722005365Raman SpectroscopyFTIR SpectroscopyEssential oilOcimum L |
| spellingShingle | Archasvi Tyagi Anil K. Yadav Akanksha Yadav Lalita Saini Vivek Kumar Pooja Jain Inam Mohammad Mohammad Javed Ansari Hesham Ali El Enshasy Fagr Kh. Abdel-Gawad Sami Al Obaid Shahida Anusha Siddiqui Vijai Malik Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric Approach Journal of King Saud University: Science Raman Spectroscopy FTIR Spectroscopy Essential oil Ocimum L |
| title | Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric Approach |
| title_full | Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric Approach |
| title_fullStr | Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric Approach |
| title_full_unstemmed | Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric Approach |
| title_short | Vibrational Spectroscopic Methods for the Identification and Distinction of Essential Oils in Genus Ocimum L.: A Chemometric Approach |
| title_sort | vibrational spectroscopic methods for the identification and distinction of essential oils in genus ocimum l a chemometric approach |
| topic | Raman Spectroscopy FTIR Spectroscopy Essential oil Ocimum L |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722005365 |
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