Molecular and Phenotypic Identification and Speciation of Malassezia Yeasts Isolated from Egyptian Patients with Pityriasis Versicolor
Introduction: Pityriasis Versicolor (PV) is a common health problem caused by genus Malassezia, a lipophilic fungi found as a part of the normal flora of skin. Although PV is common in Egypt, there is little information regarding the Malassezia species distribution in PV patients to date. Aim:...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/10416/27747_CE[Ra]_F(Sh)_PF1_(SY_MJ_PY)_PFA(SY_SS).pdf |
| Summary: | Introduction: Pityriasis Versicolor (PV) is a common health
problem caused by genus Malassezia, a lipophilic fungi found
as a part of the normal flora of skin. Although PV is common
in Egypt, there is little information regarding the Malassezia
species distribution in PV patients to date.
Aim: To spot a light on the distribution and clinico-epidemiological
features of the Malassezia species in PV patients and healthy
individuals that were established by conventional phenotypic
and molecular techniques.
Materials and Methods: A cross-sectional study including
167 individuals; 137 clinically suspected PV patients attending
Mansoura University Hospitals, Egypt and 30 healthy control
individuals, was carried out. Characterization of Malassezia
species was performed phenotypically by conventional,
culture-based methods and biochemical tests. Genomic DNA
was extracted from isolated colonies for PCR amplification of
the highly conserved 26S rDNA region with further species level
identification by Restriction Fragment Length Polymorphism
(RFLP) using Hha1 and BstC1 enzymes. The association of
Malassezia species with epidemiological profile and clinical
characteristics was studied.
Results: A 94.2% of PV samples and 13.3% of control samples
were positive by Potassium Hydroxide (KOH) while 71.5% of
PV samples and 16.7% of control samples yielded growth in
culture with high statistically significant differences (p=0.0001,
for both methods). By phenotypic methods, only 75.5% of
isolates from patients were identified as: M. furfur (51.4%), M.
globosa, (29.7%), M. restricta (13.5%) and M. pachydermatis
(5.4%) while by RFLP technique, six species were revealed:
M. furfur (44.9%), M. globosa (24.5%), M. sympodialis (12.2 %),
M. restricta (10.2%), M. obtusa (4.1%) and M. pachydermatis
(4.1%). Most species were isolated from hypopigmented lesions
of PV patients aged between 20-29 years. Neck and back were
the most common affected sites. Only M. furfur (10%) and M.
globosa (6.7%) were identified in healthy controls.
Conclusion: M. furfur and M. globosa are the commonly
encountered species in both healthy and diseased human skin
although other species were identified in PV patients. PCRRFLP method represents a considerably accurate technique
in identification of different Malassezia species for better
understanding of their effect on the clinico-epidemiological
characterization of PV patients in Egypt. |
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| ISSN: | 2249-782X 0973-709X |