Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing
Semiconducting polymer dots (Pdots) are rapidly becoming one of the most studied nanoparticles in fluorescence bioimaging and sensing. Their small size, high brightness, and resistance to photobleaching make them one of the most attractive fluorophores for fluorescence imaging and sensing applicatio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/19/7218 |
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| author | Connor Riahin Kushani Mendis Brandon Busick Marcin Ptaszek Mengran Yang Gary Stacey Amar Parvate James E. Evans Jeremiah Traeger Dehong Hu Galya Orr Zeev Rosenzweig |
| author_facet | Connor Riahin Kushani Mendis Brandon Busick Marcin Ptaszek Mengran Yang Gary Stacey Amar Parvate James E. Evans Jeremiah Traeger Dehong Hu Galya Orr Zeev Rosenzweig |
| author_sort | Connor Riahin |
| collection | DOAJ |
| description | Semiconducting polymer dots (Pdots) are rapidly becoming one of the most studied nanoparticles in fluorescence bioimaging and sensing. Their small size, high brightness, and resistance to photobleaching make them one of the most attractive fluorophores for fluorescence imaging and sensing applications. This paper highlights our recent advances in fluorescence bioimaging and sensing with nanoscale luminescent Pdots, specifically the use of organic dyes as dopant molecules to modify the optical properties of Pdots to enable deep red and near infrared fluorescence bioimaging applications and to impart sensitivity of dye doped Pdots towards selected analytes. Building on our earlier work, we report the formation of secondary antibody-conjugated Pdots and provide Cryo-TEM evidence for their formation. We demonstrate the selective targeting of the antibody-conjugated Pdots to FLAG-tagged FLS2 membrane receptors in genetically engineered plant leaf cells. We also report the formation of a new class of luminescent Pdots with emission wavelengths of around 1000 nm. Finally, we demonstrate the formation and utility of oxygen sensing Pdots in aqueous media. |
| first_indexed | 2024-03-09T21:11:05Z |
| format | Article |
| id | doaj.art-614197c228634cccaa682fa5df5633ca |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T21:11:05Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-614197c228634cccaa682fa5df5633ca2023-11-23T21:45:41ZengMDPI AGSensors1424-82202022-09-012219721810.3390/s22197218Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and SensingConnor Riahin0Kushani Mendis1Brandon Busick2Marcin Ptaszek3Mengran Yang4Gary Stacey5Amar Parvate6James E. Evans7Jeremiah Traeger8Dehong Hu9Galya Orr10Zeev Rosenzweig11Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21250, USADepartment of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21250, USADepartment of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21250, USADepartment of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21250, USADivisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, MO 65211, USADivisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, MO 65211, USAEnvironmental Molecular Sciences Laboratory (EMSL), Pacific Northwest National Laboratory, Richland, WA 99354, USAEnvironmental Molecular Sciences Laboratory (EMSL), Pacific Northwest National Laboratory, Richland, WA 99354, USAEnvironmental Molecular Sciences Laboratory (EMSL), Pacific Northwest National Laboratory, Richland, WA 99354, USAEnvironmental Molecular Sciences Laboratory (EMSL), Pacific Northwest National Laboratory, Richland, WA 99354, USAEnvironmental Molecular Sciences Laboratory (EMSL), Pacific Northwest National Laboratory, Richland, WA 99354, USADepartment of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore, MD 21250, USASemiconducting polymer dots (Pdots) are rapidly becoming one of the most studied nanoparticles in fluorescence bioimaging and sensing. Their small size, high brightness, and resistance to photobleaching make them one of the most attractive fluorophores for fluorescence imaging and sensing applications. This paper highlights our recent advances in fluorescence bioimaging and sensing with nanoscale luminescent Pdots, specifically the use of organic dyes as dopant molecules to modify the optical properties of Pdots to enable deep red and near infrared fluorescence bioimaging applications and to impart sensitivity of dye doped Pdots towards selected analytes. Building on our earlier work, we report the formation of secondary antibody-conjugated Pdots and provide Cryo-TEM evidence for their formation. We demonstrate the selective targeting of the antibody-conjugated Pdots to FLAG-tagged FLS2 membrane receptors in genetically engineered plant leaf cells. We also report the formation of a new class of luminescent Pdots with emission wavelengths of around 1000 nm. Finally, we demonstrate the formation and utility of oxygen sensing Pdots in aqueous media.https://www.mdpi.com/1424-8220/22/19/7218near infrared emissionpolymer dotsplant cell imagingsensing |
| spellingShingle | Connor Riahin Kushani Mendis Brandon Busick Marcin Ptaszek Mengran Yang Gary Stacey Amar Parvate James E. Evans Jeremiah Traeger Dehong Hu Galya Orr Zeev Rosenzweig Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing Sensors near infrared emission polymer dots plant cell imaging sensing |
| title | Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing |
| title_full | Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing |
| title_fullStr | Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing |
| title_full_unstemmed | Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing |
| title_short | Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing |
| title_sort | near infrared emitting semiconductor polymer dots for bioimaging and sensing |
| topic | near infrared emission polymer dots plant cell imaging sensing |
| url | https://www.mdpi.com/1424-8220/22/19/7218 |
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