Characterization of <i>Neopestalotiopsis</i> Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico
Mango is one of the most popular and nutritious fruits in the world and Mexico is the world’s largest exporter. There are many diseases that directly affect fruit yield and quality. During the period 2016–2017, leaves with grey leaf spots were collected from 28 commercial mango orchards distributed...
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MDPI AG
2020-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/10/788 |
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| author | Saida S. Gerardo-Lugo Juan M. Tovar-Pedraza Sajeewa S. N. Maharachchikumbura Miguel A. Apodaca-Sánchez Kamila C. Correia Carlos P. Sauceda-Acosta Moisés Camacho-Tapia Kevin D. Hyde Najat Marraiki Abdallah M. Elgorban Hugo Beltrán-Peña |
| author_facet | Saida S. Gerardo-Lugo Juan M. Tovar-Pedraza Sajeewa S. N. Maharachchikumbura Miguel A. Apodaca-Sánchez Kamila C. Correia Carlos P. Sauceda-Acosta Moisés Camacho-Tapia Kevin D. Hyde Najat Marraiki Abdallah M. Elgorban Hugo Beltrán-Peña |
| author_sort | Saida S. Gerardo-Lugo |
| collection | DOAJ |
| description | Mango is one of the most popular and nutritious fruits in the world and Mexico is the world’s largest exporter. There are many diseases that directly affect fruit yield and quality. During the period 2016–2017, leaves with grey leaf spots were collected from 28 commercial mango orchards distributed in two main production areas in Sinaloa State of Mexico, and 50 <i>Neopestalotiopsis</i> isolates were obtained. Fungal identification of 20 representative isolates was performed using morphological characterization and phylogenetic analysis based on the internal transcribed spacer (ITS) region of ribosomal DNA, part of the translation elongation factor 1-alpha (TEF) and the β-tubulin (TUB) genes. Phylogenetic analysis indicated that the 20 isolates from this study formed four consistent groups, however, overall tree topologies do not consistently provide a stable and sufficient resolution. Therefore, even though morphological and phylogenetic separation is evident, these isolates were not assigned to any new taxa and were tentatively placed into four clades (clades A–D). Pathogenicity tests on detached mango leaves of cv. Kent showed that the 20 isolates that belong to the four <i>Neopestalotiopsis</i> clades from this study and induce lesions on mango leaves. This is the first report of species of <i>Neopestalotiopsis</i> causing mango grey leaf spot disease in Mexico. |
| first_indexed | 2024-03-10T16:02:40Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T16:02:40Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-614bebab07d4485287d900b43bf0d4362023-11-20T15:07:09ZengMDPI AGPathogens2076-08172020-09-0191078810.3390/pathogens9100788Characterization of <i>Neopestalotiopsis</i> Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, MexicoSaida S. Gerardo-Lugo0Juan M. Tovar-Pedraza1Sajeewa S. N. Maharachchikumbura2Miguel A. Apodaca-Sánchez3Kamila C. Correia4Carlos P. Sauceda-Acosta5Moisés Camacho-Tapia6Kevin D. Hyde7Najat Marraiki8Abdallah M. Elgorban9Hugo Beltrán-Peña10Facultad de Agronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa 80090, MexicoLaboratorio de Fitopatología, Coordinación Culiacán, Centro de Investigación en Alimentación y Desarrollo, Culiacán, Sinaloa 80110, MexicoSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, ChinaDepartamento de Ciencias Naturales y Exactas, Unidad Regional Los Mochis, Universidad Autónoma de Occidente, Los Mochis, Sinaloa 81223, MexicoCentro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, Crato, Ceará 63.133-610, BrazilFacultad de Agricultura del Valle del Fuerte, Universidad Autónoma de Sinaloa, Ahome, Sinaloa 81110, MexicoLaboratorio Nacional de Investigación y Servicio Agroalimentario y Forestal, Universidad Autónoma Chapingo, Texcoco 56230, Estado de México, MexicoCenter of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, ThailandDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartamento de Ciencias Naturales y Exactas, Unidad Regional Los Mochis, Universidad Autónoma de Occidente, Los Mochis, Sinaloa 81223, MexicoMango is one of the most popular and nutritious fruits in the world and Mexico is the world’s largest exporter. There are many diseases that directly affect fruit yield and quality. During the period 2016–2017, leaves with grey leaf spots were collected from 28 commercial mango orchards distributed in two main production areas in Sinaloa State of Mexico, and 50 <i>Neopestalotiopsis</i> isolates were obtained. Fungal identification of 20 representative isolates was performed using morphological characterization and phylogenetic analysis based on the internal transcribed spacer (ITS) region of ribosomal DNA, part of the translation elongation factor 1-alpha (TEF) and the β-tubulin (TUB) genes. Phylogenetic analysis indicated that the 20 isolates from this study formed four consistent groups, however, overall tree topologies do not consistently provide a stable and sufficient resolution. Therefore, even though morphological and phylogenetic separation is evident, these isolates were not assigned to any new taxa and were tentatively placed into four clades (clades A–D). Pathogenicity tests on detached mango leaves of cv. Kent showed that the 20 isolates that belong to the four <i>Neopestalotiopsis</i> clades from this study and induce lesions on mango leaves. This is the first report of species of <i>Neopestalotiopsis</i> causing mango grey leaf spot disease in Mexico.https://www.mdpi.com/2076-0817/9/10/788<i>Mangifera indica</i>morphologypathogenicityvirulencephylogeny |
| spellingShingle | Saida S. Gerardo-Lugo Juan M. Tovar-Pedraza Sajeewa S. N. Maharachchikumbura Miguel A. Apodaca-Sánchez Kamila C. Correia Carlos P. Sauceda-Acosta Moisés Camacho-Tapia Kevin D. Hyde Najat Marraiki Abdallah M. Elgorban Hugo Beltrán-Peña Characterization of <i>Neopestalotiopsis</i> Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico Pathogens <i>Mangifera indica</i> morphology pathogenicity virulence phylogeny |
| title | Characterization of <i>Neopestalotiopsis</i> Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico |
| title_full | Characterization of <i>Neopestalotiopsis</i> Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico |
| title_fullStr | Characterization of <i>Neopestalotiopsis</i> Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico |
| title_full_unstemmed | Characterization of <i>Neopestalotiopsis</i> Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico |
| title_short | Characterization of <i>Neopestalotiopsis</i> Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico |
| title_sort | characterization of i neopestalotiopsis i species associated with mango grey leaf spot disease in sinaloa mexico |
| topic | <i>Mangifera indica</i> morphology pathogenicity virulence phylogeny |
| url | https://www.mdpi.com/2076-0817/9/10/788 |
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