Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato
Anthocyanins are pigmented secondary metabolites produced via the flavonoid biosynthetic pathway and play important roles in plant stress responses, pollinator attraction, and consumer preference. Using RNA-sequencing analysis of a cross between diploid potato (Solanum tuberosum L.) lines segregatin...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2020-10-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.120.401684 |
| _version_ | 1818657613917716480 |
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| author | F. Parker E. Laimbeer Bastiaan O.R. Bargmann Sarah H. Holt Trenton Pratt Brenda Peterson Andreas G. Doulis C. Robin Buell Richard E. Veilleux |
| author_facet | F. Parker E. Laimbeer Bastiaan O.R. Bargmann Sarah H. Holt Trenton Pratt Brenda Peterson Andreas G. Doulis C. Robin Buell Richard E. Veilleux |
| author_sort | F. Parker E. Laimbeer |
| collection | DOAJ |
| description | Anthocyanins are pigmented secondary metabolites produced via the flavonoid biosynthetic pathway and play important roles in plant stress responses, pollinator attraction, and consumer preference. Using RNA-sequencing analysis of a cross between diploid potato (Solanum tuberosum L.) lines segregating for flower color, we identified a homolog of the ANTHOCYANIN 2 (AN2) gene family that encodes a MYB transcription factor, herein termed StFlAN2, as the regulator of anthocyanin production in potato corollas. Transgenic introduction of StFlAN2 in white-flowered homozygous doubled-monoploid plants resulted in a recovery of purple flowers. RNA-sequencing revealed the specific anthocyanin biosynthetic genes activated by StFlAN2 as well as expression differences in genes within pathways involved in fruit ripening, senescence, and primary metabolism. Closer examination of the locus using genomic sequence analysis revealed a duplication in the StFlAN2 locus closely associated with gene expression that is likely attributable to nearby genetic elements. Taken together, this research provides insight into the regulation of anthocyanin biosynthesis in potato while also highlighting how the dynamic nature of the StFlAN2 locus may affect expression. |
| first_indexed | 2024-12-17T03:44:16Z |
| format | Article |
| id | doaj.art-614c370ab5db43bcadd5213d620706a1 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-17T03:44:16Z |
| publishDate | 2020-10-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-614c370ab5db43bcadd5213d620706a12022-12-21T22:04:55ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362020-10-0110103871387910.1534/g3.120.40168437Characterization of the F Locus Responsible for Floral Anthocyanin Production in PotatoF. Parker E. LaimbeerBastiaan O.R. BargmannSarah H. HoltTrenton PrattBrenda PetersonAndreas G. DoulisC. Robin BuellRichard E. VeilleuxAnthocyanins are pigmented secondary metabolites produced via the flavonoid biosynthetic pathway and play important roles in plant stress responses, pollinator attraction, and consumer preference. Using RNA-sequencing analysis of a cross between diploid potato (Solanum tuberosum L.) lines segregating for flower color, we identified a homolog of the ANTHOCYANIN 2 (AN2) gene family that encodes a MYB transcription factor, herein termed StFlAN2, as the regulator of anthocyanin production in potato corollas. Transgenic introduction of StFlAN2 in white-flowered homozygous doubled-monoploid plants resulted in a recovery of purple flowers. RNA-sequencing revealed the specific anthocyanin biosynthetic genes activated by StFlAN2 as well as expression differences in genes within pathways involved in fruit ripening, senescence, and primary metabolism. Closer examination of the locus using genomic sequence analysis revealed a duplication in the StFlAN2 locus closely associated with gene expression that is likely attributable to nearby genetic elements. Taken together, this research provides insight into the regulation of anthocyanin biosynthesis in potato while also highlighting how the dynamic nature of the StFlAN2 locus may affect expression.http://g3journal.org/lookup/doi/10.1534/g3.120.401684solanum tuberosumbulk segregant analysiscopy number variationtransgenic complementationmonoploid |
| spellingShingle | F. Parker E. Laimbeer Bastiaan O.R. Bargmann Sarah H. Holt Trenton Pratt Brenda Peterson Andreas G. Doulis C. Robin Buell Richard E. Veilleux Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato G3: Genes, Genomes, Genetics solanum tuberosum bulk segregant analysis copy number variation transgenic complementation monoploid |
| title | Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato |
| title_full | Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato |
| title_fullStr | Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato |
| title_full_unstemmed | Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato |
| title_short | Characterization of the F Locus Responsible for Floral Anthocyanin Production in Potato |
| title_sort | characterization of the f locus responsible for floral anthocyanin production in potato |
| topic | solanum tuberosum bulk segregant analysis copy number variation transgenic complementation monoploid |
| url | http://g3journal.org/lookup/doi/10.1534/g3.120.401684 |
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